Annotations: 1KFX

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A [auth L]	d1kfxl1	All alpha proteins	EF Hand-like	EF-hand	Penta-EF-hand proteins	Calpain large subunit, C-terminal domain (domain IV)	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
A [auth L]	d1kfxl2	All beta proteins	Calpain large subunit, middle domain (domain III)	Calpain large subunit, middle domain (domain III)	Calpain large subunit, middle domain (domain III)	Calpain large subunit, middle domain (domain III)	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
A [auth L]	d1kfxl3	Alpha and beta proteins (a+b)	Cysteine proteinases	Cysteine proteinases	Calpain large subunit, catalytic domain (domain II)	Calpain large subunit, catalytic domain (domain II)	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B [auth S]	d1kfxs_	All alpha proteins	EF Hand-like	EF-hand	Penta-EF-hand proteins	Calpain small (regulatory) subunit (domain VI)	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A [auth L]	SCOP2B Superfamily	Calpain large subunit middle domain (domain III)	8038195	3001233	SCOP2B (2022-06-29)
A [auth L]	SCOP2B Superfamily	Cysteine proteinases	8038197	3001808	SCOP2B (2022-06-29)
A [auth L]	SCOP2B Superfamily	EF-hand	8038190	3001983	SCOP2B (2022-06-29)
B [auth S]	SCOP2B Superfamily	EF-hand	8038199	3001983	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A [auth L]	PF01067	e1kfxL7	A: beta sandwiches	X: jelly-roll	H: Galactose-binding domain-like (From Topology)	T: Galactose-binding domain-like	F: PF01067	ECOD (1.6)
A [auth L]	F_UNCLASSIFIED	e1kfxL2	A: alpha arrays	X: EF-hand	H: EF-hand-related	T: EF-hand	F: F_UNCLASSIFIED	ECOD (1.6)
A [auth L]	PF13833	e1kfxL6	A: alpha arrays	X: EF-hand	H: EF-hand-related	T: EF-hand	F: PF13833	ECOD (1.6)
A [auth L]	PF00648	e1kfxL5	A: a+b complex topology	X: Cysteine proteinases-like	H: Cysteine proteinases (From Topology)	T: Cysteine proteinases	F: PF00648	ECOD (1.6)
B [auth S]	F_UNCLASSIFIED	e1kfxS1	A: alpha arrays	X: EF-hand	H: EF-hand-related	T: EF-hand	F: F_UNCLASSIFIED	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A [auth L]	3.90.70.10	Alpha Beta	Alpha-Beta Complex	Cathepsin B	Chain A	CATH (4.3.0)
A [auth L]	2.60.120.380	Mainly Beta	Sandwich	Jelly Rolls		CATH (4.3.0)
A [auth L]	1.10.238.10	Mainly Alpha	Orthogonal Bundle	Recoverin	domain 1	CATH (4.3.0)
B [auth S]	1.10.238.10	Mainly Alpha	Orthogonal Bundle	Recoverin	domain 1	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A [auth L]	PF13833	EF-hand domain pair (EF-hand_8)	EF-hand domain pair	-	Domain
A [auth L]	PF00648	Calpain family cysteine protease (Peptidase_C2)	Calpain family cysteine protease	-	Family
A [auth L]	PF01067	Calpain large subunit, domain III (Calpain_III)	Calpain large subunit, domain III	The function of the domain III and I are currently unknown. Domain II is a cysteine protease and domain IV is a calcium binding domain. Calpains are believed to participate in intracellular signaling pathways mediated by calcium ions.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth L]	M-CALPAIN LARGE SUBUNIT	cysteine-type peptidase activity peptidase activity calcium-dependent cysteine-type endopeptidase activity catalytic activity, acting on a protein cysteine-type endopeptidase activity hydrolase activity catalytic activity endopeptidase activity cytoskeletal protein binding protein binding binding protein-containing complex binding cation binding ion binding cysteine-type peptidase activity peptidase activity calcium-dependent cysteine-type endopeptidase activity catalytic activity, acting on a protein cysteine-type endopeptidase activity hydrolase activity catalytic activity endopeptidase activity cytoskeletal protein binding protein binding binding protein-containing complex binding cation binding ion binding calcium ion binding metal ion binding small molecule binding enzyme binding	organonitrogen compound metabolic process cellular biosynthetic process cellular metabolic process primary metabolic process gene expression macromolecule biosynthetic process protein maturation organic substance metabolic process protein autoprocessing biosynthetic process proteolysis organic substance biosynthetic process protein processing metabolic process organonitrogen compound metabolic process cellular biosynthetic process cellular metabolic process primary metabolic process gene expression macromolecule biosynthetic process protein maturation organic substance metabolic process protein autoprocessing biosynthetic process proteolysis organic substance biosynthetic process protein processing metabolic process cellular process protein metabolic process macromolecule metabolic process organonitrogen compound catabolic process catabolic process proteolysis involved in protein catabolic process protein catabolic process macromolecule catabolic process organic substance catabolic process cellular response to stimulus response to interferon-beta response to organic substance response to stimulus response to cytokine cellular response to interferon-beta cellular response to chemical stimulus response to chemical cellular response to cytokine stimulus cellular response to organic substance behavior multicellular organismal process response to stress multicellular organismal response to stress response to pain behavioral response to pain cellular response to acid chemical cellular response to amino acid stimulus response to acid chemical response to amino acid developmental process cellular developmental process myoblast fusion cell-cell fusion muscle cell differentiation myotube differentiation muscle structure development syncytium formation by plasma membrane fusion anatomical structure morphogenesis cell differentiation anatomical structure formation involved in morphogenesis anatomical structure development syncytium formation striated muscle cell differentiation response to decreased oxygen levels response to hypoxia response to oxygen levels response to abiotic stimulus positive regulation of cellular component organization regulation of cellular process regulation of cellular component organization regulation of syncytium formation by plasma membrane fusion regulation of developmental process positive regulation of biological process cellular component organization positive regulation of developmental process cellular component organization or biogenesis positive regulation of myoblast fusion positive regulation of cellular process regulation of biological process biological regulation regulation of myoblast fusion positive regulation of syncytium formation by plasma membrane fusion interleukin-6 production regulation of interleukin-6 production regulation of cytokine production cytokine production regulation of multicellular organismal process regulation of biosynthetic process regulation of cellular metabolic process regulation of macromolecule metabolic process regulation of cellular biosynthetic process regulation of gene expression regulation of macromolecule biosynthetic process regulation of metabolic process response to hydrogen peroxide response to oxidative stress response to oxygen-containing compound response to reactive oxygen species response to inorganic substance multi-multicellular organism process reproductive process female pregnancy multi-organism reproductive process biological process involved in interspecies interaction between organisms response to lipid response to other organism cellular response to lipopolysaccharide response to external stimulus cellular response to molecule of bacterial origin response to lipopolysaccharide cellular response to oxygen-containing compound response to biotic stimulus cellular response to biotic stimulus response to bacterium cellular response to lipid response to external biotic stimulus response to molecule of bacterial origin regulation of cytoskeleton organization regulation of organelle organization organelle organization cytoskeleton organization positive regulation of cardiac muscle cell apoptotic process positive regulation of programmed cell death muscle cell apoptotic process positive regulation of striated muscle cell apoptotic process regulation of apoptotic process regulation of cardiac muscle cell apoptotic process regulation of muscle cell apoptotic process regulation of programmed cell death cell death positive regulation of apoptotic process cardiac muscle cell apoptotic process positive regulation of muscle cell apoptotic process regulation of striated muscle cell apoptotic process striated muscle cell apoptotic process apoptotic process programmed cell death regulation of lipid metabolic process organonitrogen compound biosynthetic process positive regulation of phosphate metabolic process regulation of primary metabolic process positive regulation of metabolic process positive regulation of phosphatidylcholine biosynthetic process regulation of phosphate metabolic process phosphate-containing compound metabolic process positive regulation of lipid metabolic process phosphatidylcholine metabolic process phosphorus metabolic process positive regulation of phosphorus metabolic process phospholipid biosynthetic process regulation of phospholipid biosynthetic process glycerolipid biosynthetic process organophosphate biosynthetic process cellular lipid metabolic process regulation of phosphorus metabolic process glycerolipid metabolic process regulation of phosphatidylcholine biosynthetic process positive regulation of phosphatidylcholine metabolic process glycerophospholipid metabolic process lipid biosynthetic process positive regulation of cellular metabolic process phospholipid metabolic process positive regulation of biosynthetic process glycerophospholipid biosynthetic process regulation of phosphatidylcholine metabolic process positive regulation of phospholipid biosynthetic process regulation of phospholipid metabolic process positive regulation of phospholipid metabolic process positive regulation of lipid biosynthetic process organophosphate metabolic process regulation of lipid biosynthetic process phosphatidylcholine biosynthetic process positive regulation of cellular biosynthetic process lipid metabolic process response to mechanical stimulus multicellular organism development embryo development ending in birth or egg hatching chordate embryonic development embryo development in utero embryonic development blastocyst development	cell projection pseudopodium cellular anatomical entity plasma membrane bounded cell projection cell periphery cell surface membrane plasma membrane external side of plasma membrane side of membrane extracellular exosome membrane-bounded organelle extracellular vesicle extracellular organelle cell projection pseudopodium cellular anatomical entity plasma membrane bounded cell projection cell periphery cell surface membrane plasma membrane external side of plasma membrane side of membrane extracellular exosome membrane-bounded organelle extracellular vesicle extracellular organelle extracellular region organelle extracellular space vesicle extracellular membrane-bounded organelle intracellular anatomical structure cytoplasm cytosol somatodendritic compartment dendrite dendritic tree neuron projection intracellular organelle protein-containing complex protein-DNA complex intracellular non-membrane-bounded organelle chromatin chromosome non-membrane-bounded organelle intracellular membrane-bounded organelle endomembrane system perinuclear endoplasmic reticulum perinuclear region of cytoplasm endoplasmic reticulum Golgi apparatus catalytic complex caspase complex peptidase complex calpain complex endopeptidase complex cell body neuronal cell body anchoring junction cell junction focal adhesion cell-substrate junction membrane microdomain membrane raft lytic vacuole vacuole lysosome cell cortex cortical cytoskeleton actin cytoskeleton cytoskeleton cortical actin cytoskeleton nucleus
B [auth S]	M-CALPAIN SMALL SUBUNIT	cation binding ion binding binding calcium ion binding metal ion binding small molecule binding cysteine-type peptidase activity peptidase activity calcium-dependent cysteine-type endopeptidase activity catalytic activity, acting on a protein cysteine-type endopeptidase activity hydrolase activity catalytic activity endopeptidase activity	regulation of biological process cell population proliferation regulation of cellular process positive regulation of cell population proliferation regulation of cell population proliferation biological regulation positive regulation of biological process cellular process positive regulation of cellular process catabolic process macroautophagy cellular metabolic process autophagy regulation of cellular metabolic process regulation of biological process cell population proliferation regulation of cellular process positive regulation of cell population proliferation regulation of cell population proliferation biological regulation positive regulation of biological process cellular process positive regulation of cellular process catabolic process macroautophagy cellular metabolic process autophagy regulation of cellular metabolic process regulation of catabolic process regulation of autophagy cellular catabolic process regulation of macroautophagy regulation of cellular catabolic process metabolic process regulation of metabolic process process utilizing autophagic mechanism organic substance metabolic process organonitrogen compound metabolic process proteolysis primary metabolic process protein metabolic process macromolecule metabolic process	membrane cellular anatomical entity protein-containing complex intracellular anatomical structure catalytic complex caspase complex peptidase complex calpain complex endopeptidase complex cytoplasm extracellular exosome membrane-bounded organelle extracellular vesicle extracellular organelle membrane cellular anatomical entity protein-containing complex intracellular anatomical structure catalytic complex caspase complex peptidase complex calpain complex endopeptidase complex cytoplasm extracellular exosome membrane-bounded organelle extracellular vesicle extracellular organelle extracellular region organelle extracellular space vesicle extracellular membrane-bounded organelle cytosol cell periphery plasma membrane

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A [auth L]	IPR022684	Peptidase C2, calpain family	Family
A [auth L]	IPR038765	Papain-like cysteine peptidase superfamily	Homologous Superfamily
A [auth L]	IPR002048	EF-hand domain	Domain
A [auth L]	IPR042736	CAPN2, penta-EF hand, calcium binding motif	Domain
A [auth L]	IPR018247	EF-Hand 1, calcium-binding site	Binding Site
A [auth L]	IPR033883	Calpain subdomain III	Domain
A [auth L]	IPR036213	Calpain large subunit, domain III superfamily	Homologous Superfamily
A [auth L]	IPR022682	Peptidase C2, calpain, large subunit, domain III	Domain
A [auth L]	IPR000169	Cysteine peptidase, cysteine active site	Active Site
A [auth L]	IPR011992	EF-hand domain pair	Homologous Superfamily
A [auth L]	IPR001300	Peptidase C2, calpain, catalytic domain	Domain
A [auth L]	IPR022683	Peptidase C2, calpain, domain III	Domain
B [auth S]	IPR018247	EF-Hand 1, calcium-binding site	Binding Site
B [auth S]	IPR002048	EF-hand domain	Domain
B [auth S]	IPR011992	EF-hand domain pair	Homologous Superfamily

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A [auth L]	Pharos: P17655	acute myeloid leukemia astrocytic tumor interstitial lung disease myopathy pancreatic ductal adenocarcinoma pilocytic astrocytoma Acute Myeloid Leukemia (AML-M2) diabetes mellitus lung cancer oligodendroglioma pancreatic intraductal papillary-mucinous neoplasm subependymal giant cell astrocytoma glioblastoma malignant ependymoma ovarian cancer acute myeloid leukemia astrocytic tumor interstitial lung disease myopathy pancreatic ductal adenocarcinoma pilocytic astrocytoma Acute Myeloid Leukemia (AML-M2) diabetes mellitus lung cancer oligodendroglioma pancreatic intraductal papillary-mucinous neoplasm subependymal giant cell astrocytoma glioblastoma malignant ependymoma ovarian cancer pancreatic intraductal papillary-mucinous neoplasm with low grade dysplasia
B [auth S]	Pharos: P04632	prostate cancer dermatomyositis myopathy diabetes mellitus ovarian cancer Prostatic Neoplasms malignant pancreatic neoplasm pancreatic ductal adenocarcinoma

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.