1K04

Crystal Structure of the Focal Adhesion Targeting Domain of Focal Adhesion Kinase

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1k04a_	All alpha proteins	Four-helical up-and-down bundle	FAT domain of focal adhesion kinase	FAT domain of focal adhesion kinase	FAT domain of focal adhesion kinase	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	FAT domain of focal adhesion kinase	8025325	4002908	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	FAT domain-like	8037704	3001881	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Focal_AT	e1k04A1	A: alpha bundles	X: Four-helical up-and-down bundle	H: FAT domain of focal adhesion kinase (From Topology)	T: FAT domain of focal adhesion kinase	F: Focal_AT	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.20.5.540	Mainly Alpha	Up-down Bundle	Single alpha-helices involved in coiled-coils or other helix-helix interfaces	Single helix bin	CATH (4.3.0)
A	1.20.120.330	Mainly Alpha	Up-down Bundle	Four Helix Bundle (Hemerythrin (Met), subunit A)	Nucleotidyltransferases domain 2	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF03623	Focal adhesion targeting region (Focal_AT)	Focal adhesion targeting region	Focal adhesion kinase (FAK) is a tyrosine kinase found in focal adhesions, intracellular signaling complexes that are formed following engagement of the extracellular matrix by integrins. The C-terminal 'focal adhesion targeting' (FAT) region is nece ...	Focal adhesion kinase (FAK) is a tyrosine kinase found in focal adhesions, intracellular signaling complexes that are formed following engagement of the extracellular matrix by integrins. The C-terminal 'focal adhesion targeting' (FAT) region is necessary and sufficient for localising FAK to focal adhesions. The crystal structure of FAT shows it forms a four-helix bundle that resembles those found in two other proteins involved in cell adhesion, alpha-catenin and vinculin [1]. The binding of FAT to the focal adhesion protein, paxillin, requires the integrity of the helical bundle, whereas binding to another focal adhesion protein, talin, does not.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	FOCAL ADHESION KINASE 1	protein phosphatase binding phosphatase binding protein binding binding enzyme binding protein tyrosine kinase activity catalytic activity, acting on a protein kinase activity protein kinase activity transferase activity catalytic activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups adenyl ribonucleotide binding protein phosphatase binding phosphatase binding protein binding binding enzyme binding protein tyrosine kinase activity catalytic activity, acting on a protein kinase activity protein kinase activity transferase activity catalytic activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding non-membrane spanning protein tyrosine kinase activity JUN kinase binding kinase binding protein kinase binding SH2 domain binding protein domain specific binding phosphoric ester hydrolase activity phosphoprotein phosphatase activity hydrolase activity, acting on ester bonds protein tyrosine phosphatase activity phosphatase activity hydrolase activity cell adhesion molecule binding protein-containing complex binding integrin binding signaling receptor binding cytoskeletal protein binding actin binding molecular function activator activity molecular function regulator activity	regulation of cellular process regulation of cellular component organization cell-substrate junction assembly cellular component organization cellular component biogenesis cell adhesion cellular component assembly cell junction assembly regulation of cell-substrate junction organization cellular component organization or biogenesis focal adhesion assembly regulation of biological process regulation of cell-substrate adhesion regulation of cell adhesion regulation of cellular process regulation of cellular component organization cell-substrate junction assembly cellular component organization cellular component biogenesis cell adhesion cellular component assembly cell junction assembly regulation of cell-substrate junction organization cellular component organization or biogenesis focal adhesion assembly regulation of biological process regulation of cell-substrate adhesion regulation of cell adhesion biological regulation cell-substrate adhesion regulation of cellular component biogenesis regulation of cell junction assembly cell junction organization cell-matrix adhesion regulation of cell-matrix adhesion cell-substrate junction organization cellular process regulation of focal adhesion assembly regulation of cell-substrate junction assembly positive regulation of response to wounding wound healing response to stimulus positive regulation of biological process regulation of response to wounding positive regulation of response to stimulus positive regulation of wound healing regulation of response to stimulus response to stress regulation of response to stress regulation of wound healing response to wounding developmental process placenta development animal organ development anatomical structure development multicellular organismal process regulation of cell motility endothelial cell migration epithelium migration tissue migration regulation of locomotion ameboidal-type cell migration regulation of cell migration regulation of endothelial cell migration cell migration regulation of multicellular organismal process epithelial cell migration cell motility regulation of epithelial cell migration locomotion cellular response to stimulus cell surface receptor signaling pathway signaling cell surface receptor protein tyrosine kinase signaling pathway cell communication signal transduction enzyme-linked receptor protein signaling pathway ephrin receptor signaling pathway regulation of substrate adhesion-dependent cell spreading substrate adhesion-dependent cell spreading negative regulation of programmed cell death regulation of apoptotic process regulation of anoikis negative regulation of biological process regulation of programmed cell death cell death negative regulation of anoikis negative regulation of apoptotic process anoikis negative regulation of cellular process apoptotic process programmed cell death establishment of cell polarity establishment or maintenance of cell polarity negative regulation of cell adhesion cell-cell adhesion negative regulation of cell-cell adhesion regulation of cell-cell adhesion protein phosphorylation organonitrogen compound metabolic process protein autophosphorylation phosphorylation cellular metabolic process primary metabolic process organic substance metabolic process macromolecule modification phosphate-containing compound metabolic process metabolic process protein metabolic process macromolecule metabolic process protein modification process phosphorus metabolic process heart development heart morphogenesis circulatory system development multicellular organism development anatomical structure morphogenesis animal organ morphogenesis system development positive regulation of locomotion macrophage migration positive regulation of chemotaxis positive regulation of cell migration taxis regulation of immune system process regulation of mononuclear cell migration positive regulation of immune system process chemotaxis regulation of leukocyte chemotaxis positive regulation of macrophage migration regulation of leukocyte migration response to external stimulus positive regulation of leukocyte chemotaxis granulocyte migration leukocyte migration response to chemical regulation of response to external stimulus macrophage chemotaxis regulation of granulocyte chemotaxis regulation of macrophage migration positive regulation of response to external stimulus positive regulation of cell motility myeloid leukocyte migration positive regulation of mononuclear cell migration positive regulation of cellular process cell chemotaxis regulation of macrophage chemotaxis positive regulation of leukocyte migration regulation of chemotaxis cellular response to chemical stimulus immune system process granulocyte chemotaxis mononuclear cell migration positive regulation of macrophage chemotaxis leukocyte chemotaxis ERBB signaling pathway epidermal growth factor receptor signaling pathway cellular response to nitrogen compound response to organic substance response to peptide hormone cellular response to growth hormone stimulus cellular response to endogenous stimulus response to hormone cellular response to organic substance cellular response to hormone stimulus growth hormone receptor signaling pathway response to endogenous stimulus response to organonitrogen compound cellular response to peptide hormone stimulus response to oxygen-containing compound response to nitrogen compound cellular response to oxygen-containing compound response to growth hormone cellular response to organonitrogen compound detection of external stimulus detection of stimulus detection of mechanical stimulus detection of muscle stretch response to mechanical stimulus response to abiotic stimulus detection of abiotic stimulus response to muscle stretch positive regulation of phosphorylation regulation of catalytic activity regulation of transferase activity positive regulation of phosphate metabolic process regulation of primary metabolic process positive regulation of metabolic process regulation of phosphate metabolic process positive regulation of macromolecule metabolic process positive regulation of protein phosphorylation positive regulation of phosphorus metabolic process regulation of protein metabolic process regulation of cellular metabolic process regulation of molecular function positive regulation of kinase activity regulation of kinase activity regulation of phosphorus metabolic process regulation of metabolic process regulation of protein modification process regulation of protein phosphorylation positive regulation of protein metabolic process positive regulation of cellular metabolic process positive regulation of molecular function positive regulation of protein modification process positive regulation of protein kinase activity positive regulation of transferase activity positive regulation of catalytic activity regulation of protein kinase activity regulation of macromolecule metabolic process regulation of phosphorylation regulation of hydrolase activity regulation of GTPase activity regulation of signaling protein-containing complex organization regulation of signal transduction signal complex assembly protein-containing complex assembly regulation of cell communication cellular response to growth factor stimulus cell surface receptor protein serine/threonine kinase signaling pathway cellular response to transforming growth factor beta stimulus response to transforming growth factor beta transforming growth factor beta receptor signaling pathway response to growth factor transforming growth factor beta receptor superfamily signaling pathway regulation of osteoblast differentiation regulation of developmental process cellular developmental process regulation of cell differentiation osteoblast differentiation ossification cell differentiation netrin-activated signaling pathway cell adhesion mediated by integrin regulation of cell adhesion mediated by integrin blood vessel morphogenesis tube development angiogenesis vasculature development tube morphogenesis anatomical structure formation involved in morphogenesis blood vessel development regulation of epithelial to mesenchymal transition positive regulation of epithelial to mesenchymal transition mesenchyme development positive regulation of developmental process positive regulation of cell differentiation tissue development epithelial to mesenchymal transition positive regulation of multicellular organismal process mesenchymal cell differentiation catabolic process proteolysis involved in protein catabolic process macromolecule catabolic process regulation of ubiquitin-dependent protein catabolic process positive regulation of ubiquitin-dependent protein catabolic process regulation of proteolysis regulation of proteolysis involved in protein catabolic process modification-dependent macromolecule catabolic process proteolysis organonitrogen compound catabolic process protein catabolic process regulation of catabolic process positive regulation of proteolysis involved in protein catabolic process positive regulation of catabolic process modification-dependent protein catabolic process positive regulation of proteolysis ubiquitin-dependent protein catabolic process organic substance catabolic process regulation of fibroblast migration positive regulation of fibroblast migration fibroblast migration integrin-mediated signaling pathway positive regulation of cell communication regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction positive regulation of signal transduction intracellular signal transduction regulation of intracellular signal transduction intracellular signaling cassette positive regulation of intracellular signal transduction positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction phosphatidylinositol 3-kinase/protein kinase B signal transduction positive regulation of signaling regulation of cytoskeleton organization regulation of organelle organization organelle organization cytoskeleton organization regulation of cell shape cell morphogenesis regulation of cell morphogenesis regulation of biological quality regulation of anatomical structure morphogenesis cell population proliferation positive regulation of cell population proliferation regulation of cell population proliferation peptidyl-tyrosine modification peptidyl-amino acid modification peptidyl-tyrosine phosphorylation regulation of leukocyte proliferation leukocyte proliferation macrophage proliferation positive regulation of leukocyte proliferation positive regulation of macrophage proliferation regulation of macrophage proliferation axonogenesis cell projection organization plasma membrane bounded cell projection morphogenesis cell development neuron projection development neurogenesis cell projection morphogenesis neuron differentiation neuron projection morphogenesis plasma membrane bounded cell projection organization axon guidance nervous system development axon development cell morphogenesis involved in neuron differentiation generation of neurons neuron projection guidance neuron development vascular endothelial growth factor receptor signaling pathway immune response-regulating cell surface receptor signaling pathway involved in phagocytosis import into cell immune response-regulating cell surface receptor signaling pathway transport Fc-gamma receptor signaling pathway involved in phagocytosis vesicle-mediated transport localization immune response-activating signaling pathway Fc receptor signaling pathway immune response-regulating signaling pathway positive regulation of immune response phagocytosis Fc-gamma receptor signaling pathway immune response immune response-activating cell surface receptor signaling pathway Fc receptor mediated stimulatory signaling pathway establishment of localization immune effector process regulation of immune response endocytosis activation of immune response positive regulation of epithelial cell migration	intracellular anatomical structure cytoplasm cellular anatomical entity cytosol intracellular organelle cytoskeleton organelle intracellular non-membrane-bounded organelle non-membrane-bounded organelle neuron spine synapse neuron projection dendritic spine plasma membrane bounded cell projection intracellular anatomical structure cytoplasm cellular anatomical entity cytosol intracellular organelle cytoskeleton organelle intracellular non-membrane-bounded organelle non-membrane-bounded organelle neuron spine synapse neuron projection dendritic spine plasma membrane bounded cell projection somatodendritic compartment cell projection postsynapse cell junction dendrite dendritic tree actin filament bundle actin cytoskeleton actomyosin stress fiber contractile actin filament bundle perinuclear region of cytoplasm membrane-bounded organelle cilium ciliary basal body microtubule organizing center microtubule cytoskeleton anchoring junction focal adhesion cell-substrate junction cell periphery cell cortex centrosome plasma membrane membrane intracellular membrane-bounded organelle nucleus

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR000299	FERM domain	Domain
A	IPR041784	FAK1/PYK2, FERM domain C-lobe	Domain
A	IPR008266	Tyrosine-protein kinase, active site	Active Site
A	IPR019748	FERM central domain	Domain
A	IPR020635	Tyrosine-protein kinase, catalytic domain	Domain
A	IPR000719	Protein kinase domain	Domain
A	IPR041390	Focal adhesion kinase, N-terminal	Domain
A	IPR035963	FERM superfamily, second domain	Homologous Superfamily
A	IPR036137	Focal adhesion kinase, targeting (FAT) domain superfamily	Homologous Superfamily
A	IPR017441	Protein kinase, ATP binding site	Binding Site
A	IPR014352	FERM/acyl-CoA-binding protein superfamily	Homologous Superfamily
A	IPR049385	FAK1-like, FERM domain C-lobe	Domain
A	IPR011993	PH-like domain superfamily	Homologous Superfamily
A	IPR001245	Serine-threonine/tyrosine-protein kinase, catalytic domain	Domain
A	IPR019749	Band 4.1 domain	Domain
A	IPR011009	Protein kinase-like domain superfamily	Homologous Superfamily
A	IPR005189	Focal adhesion kinase, targeting (FAT) domain	Domain
A	IPR029071	Ubiquitin-like domain superfamily	Homologous Superfamily

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A	Pharos: Q05397	squamous cell carcinoma rheumatoid arthritis glioblastoma non-small cell lung carcinoma atrial fibrillation giant cell glioblastoma Mammary Neoplasms, Experimental oral cavity neoplasm pancreatic ductal adenocarcinoma pancreatic intraductal papillary-mucinous neoplasm with low grade dysplasia spina bifida X-linked mixed deafness with perilymphatic gusher breast cancer hepatocellular carcinoma MALT lymphoma squamous cell carcinoma rheumatoid arthritis glioblastoma non-small cell lung carcinoma atrial fibrillation giant cell glioblastoma Mammary Neoplasms, Experimental oral cavity neoplasm pancreatic ductal adenocarcinoma pancreatic intraductal papillary-mucinous neoplasm with low grade dysplasia spina bifida X-linked mixed deafness with perilymphatic gusher breast cancer hepatocellular carcinoma MALT lymphoma osteosarcoma pancreatic intraductal papillary-mucinous carcinoma Pick disease breast disease oligodendroglioma ovarian cancer pancreatic intraductal papillary-mucinous neoplasm subependymal giant cell astrocytoma attention deficit hyperactivity disorder, inattentive type cancer metastatic melanoma

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.