1J4K

SOLUTION STRUCTURE OF THE FHA2 DOMAIN OF RAD53 COMPLEXED WITH A PHOSPHOTYROSYL PEPTIDE DERIVED FROM RAD9

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Protein Modification Annotation

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1j4ka_	All beta proteins	SMAD/FHA domain	SMAD/FHA domain	FHA domain	Phosphotyrosine binding domain of Rad53	baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ],	SCOPe (2.08)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	FHA	e1j4kA1	A: beta sandwiches	X: SMAD/FHA domain (From Topology)	H: SMAD/FHA domain (From Topology)	T: SMAD/FHA domain	F: FHA	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.60.200.20	Mainly Beta	Sandwich	Tumour Suppressor Smad4		CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00498	FHA domain (FHA)	FHA domain	-	Family
B [auth P]	PF08605	Fungal Rad9-like Rad53-binding (Rad9_Rad53_bind)	Fungal Rad9-like Rad53-binding	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	PROTEIN KINASE SPK1	DNA binding sequence-specific DNA binding sequence-specific double-stranded DNA binding double-stranded DNA binding binding organic cyclic compound binding nucleic acid binding DNA replication origin binding protein serine/threonine/tyrosine kinase activity catalytic activity, acting on a protein kinase activity protein kinase activity transferase activity catalytic activity DNA binding sequence-specific DNA binding sequence-specific double-stranded DNA binding double-stranded DNA binding binding organic cyclic compound binding nucleic acid binding DNA replication origin binding protein serine/threonine/tyrosine kinase activity catalytic activity, acting on a protein kinase activity protein kinase activity transferase activity catalytic activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups protein serine kinase activity protein tyrosine kinase activity adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding protein serine/threonine kinase activity	DNA-templated DNA replication DNA replication initiation primary metabolic process DNA metabolic process nucleic acid metabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process DNA replication organic substance metabolic process metabolic process macromolecule metabolic process protein localization localization cellular localization DNA-templated DNA replication DNA replication initiation primary metabolic process DNA metabolic process nucleic acid metabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process DNA replication organic substance metabolic process metabolic process macromolecule metabolic process protein localization localization cellular localization cellular macromolecule localization macromolecule localization cellular process cellular response to stimulus regulation of cellular process regulation of cell cycle phase transition regulation of cellular component organization response to stimulus sexual reproduction signaling regulation of reproductive process reproductive process meiotic recombination checkpoint signaling negative regulation of biological process negative regulation of cell cycle cell cycle phase transition meiotic cell cycle process intracellular signal transduction cell communication cellular component organization negative regulation of cellular process negative regulation of cell cycle phase transition meiotic nuclear division signal transduction cell cycle cellular component organization or biogenesis regulation of cell cycle negative regulation of meiotic nuclear division cell cycle process regulation of biological process regulation of meiotic cell cycle negative regulation of cellular component organization regulation of cell cycle process meiotic cell cycle organelle fission biological regulation negative regulation of cell cycle process meiotic cell cycle checkpoint signaling regulation of organelle organization cell cycle checkpoint signaling regulation of meiotic nuclear division organelle organization nuclear division negative regulation of nuclear division negative regulation of organelle organization negative regulation of reproductive process regulation of nuclear division negative regulation of meiotic cell cycle phosphorylation cellular metabolic process negative regulation of metabolic process negative regulation of phosphate metabolic process regulation of cellular metabolic process negative regulation of cellular metabolic process regulation of phosphate metabolic process phosphate-containing compound metabolic process negative regulation of phosphorus metabolic process regulation of phosphorus metabolic process regulation of phosphorylation negative regulation of phosphorylation phosphorus metabolic process regulation of metabolic process regulation of cellular response to stress regulation of signal transduction regulation of cell cycle checkpoint DNA integrity checkpoint signaling regulation of response to stimulus negative regulation of DNA damage checkpoint negative regulation of cell cycle checkpoint DNA damage checkpoint signaling negative regulation of signaling negative regulation of intracellular signal transduction positive regulation of biological process negative regulation of cell communication cellular response to stress regulation of intracellular signal transduction response to stress regulation of response to stress positive regulation of cell cycle process positive regulation of cellular process regulation of signaling negative regulation of response to stimulus negative regulation of signal transduction positive regulation of cell cycle DNA damage response positive regulation of cell cycle phase transition signal transduction in response to DNA damage regulation of cell communication regulation of DNA damage checkpoint DNA repair regulation of primary metabolic process regulation of nucleobase-containing compound metabolic process regulation of macromolecule metabolic process regulation of DNA repair regulation of DNA metabolic process nucleobase-containing small molecule metabolic process organic substance biosynthetic process small molecule metabolic process deoxyribonucleoside triphosphate metabolic process nucleoside phosphate metabolic process nucleoside triphosphate biosynthetic process nucleobase-containing compound biosynthetic process biosynthetic process organophosphate metabolic process organophosphate biosynthetic process organic cyclic compound biosynthetic process nucleoside phosphate biosynthetic process nucleoside triphosphate metabolic process deoxyribonucleoside triphosphate biosynthetic process	intracellular anatomical structure cytoplasm cellular anatomical entity cytosol membrane-bounded organelle intracellular organelle organelle nucleus intracellular membrane-bounded organelle
B [auth P]	DNA REPAIR PROTEIN RAD9	enzyme regulator activity enzyme activator activity molecular function activator activity molecular function regulator activity protein binding binding histone binding DNA binding double-stranded DNA binding organic cyclic compound binding nucleic acid binding	cellular response to stimulus response to stimulus primary metabolic process DNA metabolic process nucleic acid metabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process organic substance metabolic process cellular response to stress DNA damage response response to stress metabolic process cellular process macromolecule metabolic process cellular response to stimulus response to stimulus primary metabolic process DNA metabolic process nucleic acid metabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process organic substance metabolic process cellular response to stress DNA damage response response to stress metabolic process cellular process macromolecule metabolic process DNA repair nucleotide-excision repair double-strand break repair DNA damage checkpoint signaling regulation of cellular process regulation of cell cycle phase transition signaling negative regulation of biological process negative regulation of cell cycle cell cycle phase transition intracellular signal transduction mitotic cell cycle negative regulation of mitotic cell cycle cell communication regulation of mitotic cell cycle mitotic DNA integrity checkpoint signaling mitotic DNA damage checkpoint signaling negative regulation of cellular process negative regulation of cell cycle phase transition signal transduction cell cycle mitotic intra-S DNA damage checkpoint signaling regulation of cell cycle cell cycle process regulation of biological process regulation of cell cycle process biological regulation DNA integrity checkpoint signaling negative regulation of cell cycle process cell cycle checkpoint signaling mitotic cell cycle checkpoint signaling mitotic cell cycle process signal transduction in response to DNA damage DNA-templated DNA replication maintenance of fidelity regulation of primary metabolic process negative regulation of DNA-templated DNA replication regulation of DNA strand resection involved in replication fork processing DNA strand resection involved in replication fork processing negative regulation of nucleobase-containing compound metabolic process negative regulation of DNA strand resection involved in replication fork processing regulation of nucleobase-containing compound metabolic process negative regulation of macromolecule metabolic process DNA-templated DNA replication negative regulation of DNA replication negative regulation of metabolic process regulation of macromolecule metabolic process DNA replication regulation of DNA-templated DNA replication negative regulation of DNA metabolic process regulation of metabolic process replication fork processing regulation of DNA metabolic process regulation of DNA replication regulation of cell cycle G1/S phase transition cell cycle G1/S phase transition negative regulation of G1/S transition of mitotic cell cycle mitotic G1/S transition checkpoint signaling negative regulation of mitotic cell cycle phase transition negative regulation of cell cycle G1/S phase transition regulation of mitotic cell cycle phase transition mitotic G1 DNA damage checkpoint signaling regulation of G1/S transition of mitotic cell cycle G1/S transition of mitotic cell cycle mitotic cell cycle phase transition positive regulation of macromolecule biosynthetic process positive regulation of metabolic process DNA-templated transcription regulation of RNA metabolic process transcription by RNA polymerase II organic substance biosynthetic process positive regulation of macromolecule metabolic process regulation of transcription by RNA polymerase II positive regulation of nucleobase-containing compound metabolic process cellular metabolic process regulation of cellular metabolic process positive regulation of transcription by RNA polymerase II positive regulation of RNA metabolic process regulation of gene expression biosynthetic process organic cyclic compound biosynthetic process regulation of macromolecule biosynthetic process regulation of DNA-templated transcription positive regulation of DNA-templated transcription cellular biosynthetic process positive regulation of biological process RNA metabolic process positive regulation of cellular metabolic process RNA biosynthetic process nucleic acid biosynthetic process positive regulation of biosynthetic process positive regulation of cellular process regulation of RNA biosynthetic process regulation of biosynthetic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process regulation of cellular biosynthetic process positive regulation of RNA biosynthetic process positive regulation of cellular biosynthetic process	intracellular organelle protein-containing complex intracellular anatomical structure protein-DNA complex organelle intracellular non-membrane-bounded organelle chromatin chromosome cellular anatomical entity non-membrane-bounded organelle membrane-bounded organelle nucleus intracellular membrane-bounded organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR017441	Protein kinase, ATP binding site	Binding Site
A	IPR000719	Protein kinase domain	Domain
A	IPR008984	SMAD/FHA domain superfamily	Homologous Superfamily
A	IPR000253	Forkhead-associated (FHA) domain	Domain
A	IPR011009	Protein kinase-like domain superfamily	Homologous Superfamily
A	IPR016256	Serine/threonine-protein kinase Rad53	Family
A	IPR008271	Serine/threonine-protein kinase, active site	Active Site
B [auth P]	IPR047249	TP53-binding protein 1-like, first BRCT domain	Domain
B [auth P]	IPR013914	Rad9-like Rad53-binding domain, fungi	Domain
B [auth P]	IPR047252	TP53-binding protein 1-like	Family
B [auth P]	IPR001357	BRCT domain	Domain
B [auth P]	IPR036420	BRCT domain superfamily	Homologous Superfamily

Protein Modification Annotation

Modified Residue(s)
Chain	Residue(s)	Description
B [auth P]	PTR	Parent Component: TYR RESID: AA0039 PSI-MOD : O4'-phospho-L-tyrosine MOD:00048

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.