1I84

CRYO-EM STRUCTURE OF THE HEAVY MEROMYOSIN SUBFRAGMENT OF CHICKEN GIZZARD SMOOTH MUSCLE MYOSIN WITH REGULATORY LIGHT CHAIN IN THE DEPHOSPHORYLATED STATE. ONLY C ALPHAS PROVIDED FOR REGULATORY LIGHT CHAIN. ONLY BACKBONE ATOMS PROVIDED FOR S2 FRAGMENT.

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Protein Modification Annotation

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
D [auth V]	d1i84v_	Low resolution protein structures	Muscle protein complexes	Muscle protein complexes	Muscle protein complexes	Heavy meromyosin subfragment	chicken (Gallus gallus ) [TaxId: 9031 ],	SCOPe (2.08)
A [auth S]	d1i84s_	Low resolution protein structures	Muscle protein complexes	Muscle protein complexes	Muscle protein complexes	Heavy meromyosin subfragment	chicken (Gallus gallus ) [TaxId: 9031 ],	SCOPe (2.08)
B [auth T]	d1i84t_	Low resolution protein structures	Muscle protein complexes	Muscle protein complexes	Muscle protein complexes	Heavy meromyosin subfragment	chicken (Gallus gallus ) [TaxId: 9031 ],	SCOPe (2.08)
E [auth W]	d1i84w_	Low resolution protein structures	Muscle protein complexes	Muscle protein complexes	Muscle protein complexes	Heavy meromyosin subfragment	chicken (Gallus gallus ) [TaxId: 9031 ],	SCOPe (2.08)
C [auth U]	d1i84u_	Low resolution protein structures	Muscle protein complexes	Muscle protein complexes	Muscle protein complexes	Heavy meromyosin subfragment	chicken (Gallus gallus ) [TaxId: 9031 ],	SCOPe (2.08)
F [auth Z]	d1i84z_	Low resolution protein structures	Muscle protein complexes	Muscle protein complexes	Muscle protein complexes	Heavy meromyosin subfragment	chicken (Gallus gallus ) [TaxId: 9031 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
D [auth V]	SCOP2B Superfamily	Myosin s1 fragment N-terminal domain	8041636	3000319	SCOP2B (2022-06-29)
D [auth V]	SCOP2B Superfamily	P-loop motor domain	8044301	3002036	SCOP2B (2022-06-29)
A [auth S]	SCOP2B Superfamily	Myosin s1 fragment N-terminal domain	8041636	3000319	SCOP2B (2022-06-29)
A [auth S]	SCOP2B Superfamily	P-loop motor domain	8044301	3002036	SCOP2B (2022-06-29)
B [auth T]	SCOP2B Superfamily	EF-hand	8040884	3001983	SCOP2B (2022-06-29)
E [auth W]	SCOP2B Superfamily	EF-hand	8040884	3001983	SCOP2B (2022-06-29)
C [auth U]	SCOP2 Family	Calmodulin-like	8062902	4000924	SCOP2 (2022-06-29)
C [auth U]	SCOP2 Superfamily	EF-hand	8062903	3001983	SCOP2 (2022-06-29)
F [auth Z]	SCOP2B Superfamily	EF-hand	8062903	3001983	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
D [auth V]	Myosin_N	e1i84V1	A: beta barrels	X: SH3	H: SH3	T: SH3	F: Myosin_N	ECOD (1.6)
D [auth V]	KOG0161_1st	e1i84V3	A: alpha bundles	X: Myosin VI lever arm (From Topology)	H: Myosin VI lever arm (From Topology)	T: Myosin VI lever arm	F: KOG0161_1st	ECOD (1.6)
D [auth V]	Myosin_head	e1i84V2	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: Myosin_head	ECOD (1.6)
A [auth S]	Myosin_N	e1i84S1	A: beta barrels	X: SH3	H: SH3	T: SH3	F: Myosin_N	ECOD (1.6)
A [auth S]	KOG0161_1st	e1i84S3	A: alpha bundles	X: Myosin VI lever arm (From Topology)	H: Myosin VI lever arm (From Topology)	T: Myosin VI lever arm	F: KOG0161_1st	ECOD (1.6)
A [auth S]	Myosin_head	e1i84S2	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: Myosin_head	ECOD (1.6)
B [auth T]	EF-hand_7_8	e1i84T2	A: alpha arrays	X: EF-hand	H: EF-hand-related	T: EF-hand	F: EF-hand_7_8	ECOD (1.6)
B [auth T]	EF-hand_7_5	e1i84T1	A: alpha arrays	X: EF-hand	H: EF-hand-related	T: EF-hand	F: EF-hand_7_5	ECOD (1.6)
E [auth W]	EF-hand_7_8	e1i84W2	A: alpha arrays	X: EF-hand	H: EF-hand-related	T: EF-hand	F: EF-hand_7_8	ECOD (1.6)
E [auth W]	EF-hand_7_5	e1i84W1	A: alpha arrays	X: EF-hand	H: EF-hand-related	T: EF-hand	F: EF-hand_7_5	ECOD (1.6)
C [auth U]	EF-hand_7_8	e1i84U2	A: alpha arrays	X: EF-hand	H: EF-hand-related	T: EF-hand	F: EF-hand_7_8	ECOD (1.6)
C [auth U]	EF-hand_7_4	e1i84U1	A: alpha arrays	X: EF-hand	H: EF-hand-related	T: EF-hand	F: EF-hand_7_4	ECOD (1.6)
F [auth Z]	EF-hand_7_8	e1i84Z2	A: alpha arrays	X: EF-hand	H: EF-hand-related	T: EF-hand	F: EF-hand_7_8	ECOD (1.6)
F [auth Z]	EF-hand_7_4	e1i84Z1	A: alpha arrays	X: EF-hand	H: EF-hand-related	T: EF-hand	F: EF-hand_7_4	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A [auth S], D [auth V]	PF02736	Myosin N-terminal SH3-like domain (Myosin_N)	Myosin N-terminal SH3-like domain	This domain has an SH3-like fold. It is found at the N-terminus of many but not all myosins. The function of this domain is unknown.	Domain
A [auth S], D [auth V]	PF00063	Myosin head (motor domain) (Myosin_head)	Myosin head (motor domain)	-	Domain
A [auth S], D [auth V]	PF01576	Myosin tail (Myosin_tail_1)	Myosin tail	-	Coiled-coil

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth S], D [auth V]	SMOOTH MUSCLE MYOSIN HEAVY CHAIN	cytoskeletal protein binding actin binding protein-containing complex binding protein binding binding actin filament binding structural molecule activity structural constituent of muscle myosin II binding myosin binding myosin light chain binding cation binding magnesium ion binding ion binding cytoskeletal protein binding actin binding protein-containing complex binding protein binding binding actin filament binding structural molecule activity structural constituent of muscle myosin II binding myosin binding myosin light chain binding cation binding magnesium ion binding ion binding metal ion binding small molecule binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding ADP binding calmodulin binding cytoskeletal motor activity microfilament motor activity ATP-dependent activity	cellular developmental process cellular component assembly involved in morphogenesis non-membrane-bounded organelle assembly cell development myosin filament assembly skeletal myofibril assembly muscle cell differentiation cellular component organization cellular component biogenesis muscle structure development striated muscle cell development cellular anatomical entity morphogenesis cellular component assembly muscle cell development cell differentiation cellular developmental process cellular component assembly involved in morphogenesis non-membrane-bounded organelle assembly cell development myosin filament assembly skeletal myofibril assembly muscle cell differentiation cellular component organization cellular component biogenesis muscle structure development striated muscle cell development cellular anatomical entity morphogenesis cellular component assembly muscle cell development cell differentiation anatomical structure development actin filament-based process protein-containing complex assembly cellular component organization or biogenesis skeletal muscle myosin thick filament assembly developmental process actomyosin structure organization actin cytoskeleton organization organelle assembly protein-containing complex organization myosin filament organization anatomical structure morphogenesis striated muscle myosin thick filament assembly myofibril assembly organelle organization anatomical structure formation involved in morphogenesis cellular process striated muscle cell differentiation supramolecular fiber organization cytoskeleton organization multicellular organismal process system process smooth muscle contraction muscle contraction muscle system process cardiac muscle cell development striated muscle tissue development multicellular organism development cardiac cell development muscle tissue development system development heart development cardiac muscle tissue development animal organ development cardiac muscle cell differentiation tissue development cardiocyte differentiation circulatory system development elastic fiber assembly extracellular matrix organization extracellular matrix assembly extracellular structure organization external encapsulating structure organization	protein-containing complex intracellular anatomical structure cellular anatomical entity actin cytoskeleton myosin complex intracellular organelle cytoskeleton organelle intracellular non-membrane-bounded organelle contractile muscle fiber myosin II complex cytoplasm supramolecular fiber muscle myosin complex protein-containing complex intracellular anatomical structure cellular anatomical entity actin cytoskeleton myosin complex intracellular organelle cytoskeleton organelle intracellular non-membrane-bounded organelle contractile muscle fiber myosin II complex cytoplasm supramolecular fiber muscle myosin complex supramolecular polymer supramolecular complex non-membrane-bounded organelle myosin filament myofibril
B [auth T], E [auth W]	SMOOTH MUSCLE MYOSIN ESSENTIAL LIGHT CHAIN	cation binding magnesium ion binding ion binding binding metal ion binding small molecule binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding nucleoside phosphate binding ADP binding purine nucleotide binding organic cyclic compound binding anion binding cation binding magnesium ion binding ion binding binding metal ion binding small molecule binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding nucleoside phosphate binding ADP binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding calcium ion binding myosin heavy chain binding cytoskeletal protein binding protein binding myosin binding structural molecule activity structural constituent of muscle myosin II binding cytoskeletal motor activity microfilament motor activity ATP-dependent activity	cellular component organization organelle organization actomyosin structure organization actin cytoskeleton organization actin filament-based process cellular process cellular component organization or biogenesis cytoskeleton organization cellular developmental process cellular component assembly involved in morphogenesis non-membrane-bounded organelle assembly cell development muscle cell differentiation cellular component biogenesis cellular component organization organelle organization actomyosin structure organization actin cytoskeleton organization actin filament-based process cellular process cellular component organization or biogenesis cytoskeleton organization cellular developmental process cellular component assembly involved in morphogenesis non-membrane-bounded organelle assembly cell development muscle cell differentiation cellular component biogenesis muscle structure development striated muscle cell development cellular anatomical entity morphogenesis cellular component assembly muscle cell development cell differentiation anatomical structure development developmental process organelle assembly anatomical structure morphogenesis myofibril assembly anatomical structure formation involved in morphogenesis striated muscle cell differentiation supramolecular fiber organization	myosin II filament myosin filament supramolecular fiber cellular anatomical entity supramolecular polymer supramolecular complex protein-containing complex intracellular anatomical structure actin cytoskeleton myosin complex intracellular organelle cytoskeleton organelle intracellular non-membrane-bounded organelle myosin II filament myosin filament supramolecular fiber cellular anatomical entity supramolecular polymer supramolecular complex protein-containing complex intracellular anatomical structure actin cytoskeleton myosin complex intracellular organelle cytoskeleton organelle intracellular non-membrane-bounded organelle myosin II complex non-membrane-bounded organelle contractile muscle fiber cytoplasm muscle myosin complex actomyosin cytosol
C [auth U], F [auth Z]	SMOOTH MUSCLE MYOSIN REGULATORY LIGHT CHAIN	cation binding ion binding binding calcium ion binding metal ion binding small molecule binding	multicellular organismal process system process muscle contraction muscle system process developmental process muscle structure development skeletal muscle organ development skeletal muscle tissue development anatomical structure development animal organ development muscle tissue development muscle organ development tissue development	intracellular anatomical structure cytoplasm cellular anatomical entity intracellular organelle protein-containing complex cytoskeleton organelle intracellular non-membrane-bounded organelle actin cytoskeleton non-membrane-bounded organelle myosin complex

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A [auth S], D [auth V]	IPR036961	Kinesin motor domain superfamily	Homologous Superfamily
A [auth S], D [auth V]	IPR000048	IQ motif, EF-hand binding site	Binding Site
A [auth S], D [auth V]	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
A [auth S], D [auth V]	IPR014751	DNA repair protein XRCC4-like, C-terminal	Homologous Superfamily
A [auth S], D [auth V]	IPR001609	Myosin head, motor domain	Domain
A [auth S], D [auth V]	IPR002928	Myosin tail	Domain
A [auth S], D [auth V]	IPR004009	Myosin, N-terminal, SH3-like	Domain
A [auth S], D [auth V]	IPR008989	Myosin S1 fragment, N-terminal	Homologous Superfamily
B [auth T], E [auth W]	IPR002048	EF-hand domain	Domain
B [auth T], E [auth W]	IPR011992	EF-hand domain pair	Homologous Superfamily
C [auth U], F [auth Z]	IPR018247	EF-Hand 1, calcium-binding site	Binding Site
C [auth U], F [auth Z]	IPR002048	EF-hand domain	Domain
C [auth U], F [auth Z]	IPR011992	EF-hand domain pair	Homologous Superfamily

Protein Modification Annotation

Modified Residue(s)
Chain	Residue(s)	Description
A [auth S], D [auth V]	MLY	Parent Component: LYS RESID: AA0075 PSI-MOD : N6,N6-dimethyl-L-lysine MOD:00084

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.