1HT2

Nucleotide-Dependent Conformational Changes in a Protease-Associated ATPase HslU

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
C [auth E]	d1ht2e1	Alpha and beta proteins (a/b)	P-loop containing nucleoside triphosphate hydrolases	P-loop containing nucleoside triphosphate hydrolases	Extended AAA-ATPase domain	HslU	(Escherichia coli BL21(DE3) ) [TaxId: 469008 ],	SCOPe (2.08)
C [auth E]	d1ht2e2	Artifacts	Tags	Tags	Tags	N-terminal Tags	(Escherichia coli BL21(DE3) ) [TaxId: 469008 ],	SCOPe (2.08)
D [auth F]	d1ht2f1	Alpha and beta proteins (a/b)	P-loop containing nucleoside triphosphate hydrolases	P-loop containing nucleoside triphosphate hydrolases	Extended AAA-ATPase domain	HslU	(Escherichia coli BL21(DE3) ) [TaxId: 469008 ],	SCOPe (2.08)
D [auth F]	d1ht2f2	Artifacts	Tags	Tags	Tags	N-terminal Tags	(Escherichia coli BL21(DE3) ) [TaxId: 469008 ],	SCOPe (2.08)
I [auth G]	d1ht2g1	Alpha and beta proteins (a/b)	P-loop containing nucleoside triphosphate hydrolases	P-loop containing nucleoside triphosphate hydrolases	Extended AAA-ATPase domain	HslU	(Escherichia coli BL21(DE3) ) [TaxId: 469008 ],	SCOPe (2.08)
I [auth G]	d1ht2g2	Artifacts	Tags	Tags	Tags	N-terminal Tags	(Escherichia coli BL21(DE3) ) [TaxId: 469008 ],	SCOPe (2.08)
J [auth H]	d1ht2h1	Alpha and beta proteins (a/b)	P-loop containing nucleoside triphosphate hydrolases	P-loop containing nucleoside triphosphate hydrolases	Extended AAA-ATPase domain	HslU	(Escherichia coli BL21(DE3) ) [TaxId: 469008 ],	SCOPe (2.08)
J [auth H]	d1ht2h2	Artifacts	Tags	Tags	Tags	N-terminal Tags	(Escherichia coli BL21(DE3) ) [TaxId: 469008 ],	SCOPe (2.08)
A	d1ht2a_	Alpha and beta proteins (a+b)	Ntn hydrolase-like	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	Proteasome subunits	HslV (ClpQ) protease	(Escherichia coli BL21(DE3) ) [TaxId: 469008 ],	SCOPe (2.08)
B	d1ht2b_	Alpha and beta proteins (a+b)	Ntn hydrolase-like	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	Proteasome subunits	HslV (ClpQ) protease	(Escherichia coli BL21(DE3) ) [TaxId: 469008 ],	SCOPe (2.08)
E [auth C]	d1ht2c_	Alpha and beta proteins (a+b)	Ntn hydrolase-like	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	Proteasome subunits	HslV (ClpQ) protease	(Escherichia coli BL21(DE3) ) [TaxId: 469008 ],	SCOPe (2.08)
F [auth D]	d1ht2d_	Alpha and beta proteins (a+b)	Ntn hydrolase-like	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	Proteasome subunits	HslV (ClpQ) protease	(Escherichia coli BL21(DE3) ) [TaxId: 469008 ],	SCOPe (2.08)
G [auth I]	d1ht2i_	Alpha and beta proteins (a+b)	Ntn hydrolase-like	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	Proteasome subunits	HslV (ClpQ) protease	(Escherichia coli BL21(DE3) ) [TaxId: 469008 ],	SCOPe (2.08)
H [auth J]	d1ht2j_	Alpha and beta proteins (a+b)	Ntn hydrolase-like	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	Proteasome subunits	HslV (ClpQ) protease	(Escherichia coli BL21(DE3) ) [TaxId: 469008 ],	SCOPe (2.08)
K	d1ht2k_	Alpha and beta proteins (a+b)	Ntn hydrolase-like	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	Proteasome subunits	HslV (ClpQ) protease	(Escherichia coli BL21(DE3) ) [TaxId: 469008 ],	SCOPe (2.08)
L	d1ht2l_	Alpha and beta proteins (a+b)	Ntn hydrolase-like	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	Proteasome subunits	HslV (ClpQ) protease	(Escherichia coli BL21(DE3) ) [TaxId: 469008 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
C [auth E]	SCOP2B Superfamily	RecA-like P-loop NTPases	8044319	3002019	SCOP2B (2022-06-29)
D [auth F]	SCOP2B Superfamily	RecA-like P-loop NTPases	8044319	3002019	SCOP2B (2022-06-29)
I [auth G]	SCOP2B Superfamily	RecA-like P-loop NTPases	8044319	3002019	SCOP2B (2022-06-29)
J [auth H]	SCOP2B Superfamily	RecA-like P-loop NTPases	8044319	3002019	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Class II glutamine amidotransferases	8036886	3000131	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Class II glutamine amidotransferases	8036886	3000131	SCOP2B (2022-06-29)
E [auth C]	SCOP2B Superfamily	Class II glutamine amidotransferases	8036886	3000131	SCOP2B (2022-06-29)
F [auth D]	SCOP2B Superfamily	Class II glutamine amidotransferases	8036886	3000131	SCOP2B (2022-06-29)
G [auth I]	SCOP2B Superfamily	Class II glutamine amidotransferases	8036886	3000131	SCOP2B (2022-06-29)
H [auth J]	SCOP2B Superfamily	Class II glutamine amidotransferases	8036886	3000131	SCOP2B (2022-06-29)
K	SCOP2B Superfamily	Class II glutamine amidotransferases	8036886	3000131	SCOP2B (2022-06-29)
L	SCOP2B Superfamily	Class II glutamine amidotransferases	8036886	3000131	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
C [auth E]	F_UNCLASSIFIED	e1ht2E2	A: alpha arrays	X: Histone-like	H: Histone-related	T: AAA+ ATPase lid domain	F: F_UNCLASSIFIED	ECOD (1.6)
C [auth E]	PF07724,PF07728	e1ht2E1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: PF07724,PF07728	ECOD (1.6)
D [auth F]	F_UNCLASSIFIED	e1ht2F2	A: alpha arrays	X: Histone-like	H: Histone-related	T: AAA+ ATPase lid domain	F: F_UNCLASSIFIED	ECOD (1.6)
D [auth F]	PF07724,PF07728	e1ht2F1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: PF07724,PF07728	ECOD (1.6)
I [auth G]	F_UNCLASSIFIED	e1ht2G2	A: alpha arrays	X: Histone-like	H: Histone-related	T: AAA+ ATPase lid domain	F: F_UNCLASSIFIED	ECOD (1.6)
I [auth G]	PF07724,PF07728	e1ht2G1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: PF07724,PF07728	ECOD (1.6)
J [auth H]	F_UNCLASSIFIED	e1ht2H2	A: alpha arrays	X: Histone-like	H: Histone-related	T: AAA+ ATPase lid domain	F: F_UNCLASSIFIED	ECOD (1.6)
J [auth H]	PF07724,PF07728	e1ht2H1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: PF07724,PF07728	ECOD (1.6)
A	PF00227	e1ht2A1	A: a+b four layers	X: Ntn/PP2C	H: Ntn	T: Proteasome subunits	F: PF00227	ECOD (1.6)
B	PF00227	e1ht2B1	A: a+b four layers	X: Ntn/PP2C	H: Ntn	T: Proteasome subunits	F: PF00227	ECOD (1.6)
E [auth C]	PF00227	e1ht2C1	A: a+b four layers	X: Ntn/PP2C	H: Ntn	T: Proteasome subunits	F: PF00227	ECOD (1.6)
F [auth D]	PF00227	e1ht2D1	A: a+b four layers	X: Ntn/PP2C	H: Ntn	T: Proteasome subunits	F: PF00227	ECOD (1.6)
G [auth I]	PF00227	e1ht2I1	A: a+b four layers	X: Ntn/PP2C	H: Ntn	T: Proteasome subunits	F: PF00227	ECOD (1.6)
H [auth J]	PF00227	e1ht2J1	A: a+b four layers	X: Ntn/PP2C	H: Ntn	T: Proteasome subunits	F: PF00227	ECOD (1.6)
K	PF00227	e1ht2K1	A: a+b four layers	X: Ntn/PP2C	H: Ntn	T: Proteasome subunits	F: PF00227	ECOD (1.6)
L	PF00227	e1ht2L1	A: a+b four layers	X: Ntn/PP2C	H: Ntn	T: Proteasome subunits	F: PF00227	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
C [auth E]	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
C [auth E]	1.10.8.10	Mainly Alpha	Orthogonal Bundle	Helicase, Ruva Protein	domain 3	CATH (4.3.0)
C [auth E]	1.10.8.60	Mainly Alpha	Orthogonal Bundle	Helicase, Ruva Protein	domain 3	CATH (4.3.0)
D [auth F]	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
D [auth F]	1.10.8.10	Mainly Alpha	Orthogonal Bundle	Helicase, Ruva Protein	domain 3	CATH (4.3.0)
D [auth F]	1.10.8.60	Mainly Alpha	Orthogonal Bundle	Helicase, Ruva Protein	domain 3	CATH (4.3.0)
I [auth G]	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
I [auth G]	1.10.8.10	Mainly Alpha	Orthogonal Bundle	Helicase, Ruva Protein	domain 3	CATH (4.3.0)
I [auth G]	1.10.8.60	Mainly Alpha	Orthogonal Bundle	Helicase, Ruva Protein	domain 3	CATH (4.3.0)
J [auth H]	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
J [auth H]	1.10.8.10	Mainly Alpha	Orthogonal Bundle	Helicase, Ruva Protein	domain 3	CATH (4.3.0)
J [auth H]	1.10.8.60	Mainly Alpha	Orthogonal Bundle	Helicase, Ruva Protein	domain 3	CATH (4.3.0)
A	3.60.20.10	Alpha Beta	4-Layer Sandwich	Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1	Aminohydrolase, N-terminal nucleophile (Ntn) domain	CATH (4.3.0)
B	3.60.20.10	Alpha Beta	4-Layer Sandwich	Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1	Aminohydrolase, N-terminal nucleophile (Ntn) domain	CATH (4.3.0)
E [auth C]	3.60.20.10	Alpha Beta	4-Layer Sandwich	Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1	Aminohydrolase, N-terminal nucleophile (Ntn) domain	CATH (4.3.0)
F [auth D]	3.60.20.10	Alpha Beta	4-Layer Sandwich	Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1	Aminohydrolase, N-terminal nucleophile (Ntn) domain	CATH (4.3.0)
G [auth I]	3.60.20.10	Alpha Beta	4-Layer Sandwich	Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1	Aminohydrolase, N-terminal nucleophile (Ntn) domain	CATH (4.3.0)
H [auth J]	3.60.20.10	Alpha Beta	4-Layer Sandwich	Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1	Aminohydrolase, N-terminal nucleophile (Ntn) domain	CATH (4.3.0)
K	3.60.20.10	Alpha Beta	4-Layer Sandwich	Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1	Aminohydrolase, N-terminal nucleophile (Ntn) domain	CATH (4.3.0)
L	3.60.20.10	Alpha Beta	4-Layer Sandwich	Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1	Aminohydrolase, N-terminal nucleophile (Ntn) domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
C [auth E], D [auth F], I [auth G], J [auth H]	PF00004	ATPase family associated with various cellular activities (AAA) (AAA)	ATPase family associated with various cellular activities (AAA)	AAA family proteins often perform chaperone-like functions that assist in the assembly, operation, or disassembly of protein complexes [2].	Domain
A, B, E [auth C], F [auth D], G [auth I], A, B, E [auth C], F [auth D], G [auth I], H [auth J], K, L	PF00227	Proteasome subunit (Proteasome)	Proteasome subunit	The proteasome is a multisubunit structure that degrades proteins. Protein degradation is an essential component of regulation because proteins can become misfolded, damaged, or unnecessary. Proteasomes and their homologues vary greatly in complexity ...	The proteasome is a multisubunit structure that degrades proteins. Protein degradation is an essential component of regulation because proteins can become misfolded, damaged, or unnecessary. Proteasomes and their homologues vary greatly in complexity: from HslV (heat shock locus v), which is encoded by 1 gene in bacteria, to the eukaryotic 20S proteasome, which is encoded by more than 14 genes [1]. Recently evidence of two novel groups of bacterial proteasomes was proposed. The first is Anbu, which is sparsely distributed among cyanobacteria and proteobacteria [1]. The second is call beta-proteobacteria proteasome homologue (BPH) [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
C [auth E], D [auth F], I [auth G], J [auth H]	HEAT SHOCK LOCUS HSLU	cation binding magnesium ion binding ion binding binding metal ion binding small molecule binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding purine nucleotide binding organic cyclic compound binding anion binding cation binding magnesium ion binding ion binding binding metal ion binding small molecule binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding protein binding protein domain specific binding peptidase activity catalytic activity, acting on a protein hydrolase activity catalytic activity pyrophosphatase activity ATP hydrolysis activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity, acting on acid anhydrides ATP-dependent activity identical protein binding proteasome-activating activity	cellular process protein unfolding cellular response to stimulus cellular response to heat response to stimulus cellular response to stress response to stress response to heat response to abiotic stimulus response to temperature stimulus organonitrogen compound catabolic process protein denaturation organonitrogen compound metabolic process catabolic process cellular process protein unfolding cellular response to stimulus cellular response to heat response to stimulus cellular response to stress response to stress response to heat response to abiotic stimulus response to temperature stimulus organonitrogen compound catabolic process protein denaturation organonitrogen compound metabolic process catabolic process primary metabolic process protein catabolic process macromolecule catabolic process organic substance metabolic process metabolic process protein metabolic process macromolecule metabolic process organic substance catabolic process proteolysis involved in protein catabolic process proteolysis	membrane cellular anatomical entity protein-containing complex intracellular anatomical structure catalytic complex peptidase complex cytosolic proteasome complex HslUV protease complex proteasome complex endopeptidase complex cytoplasm cytosol intracellular protein-containing complex
A, B, E [auth C], F [auth D], G [auth I], A, B, E [auth C], F [auth D], G [auth I], H [auth J], K, L	HEAT SHOCK LOCUS HSLV	cation binding magnesium ion binding ion binding binding metal ion binding small molecule binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding purine nucleotide binding organic cyclic compound binding anion binding cation binding magnesium ion binding ion binding binding metal ion binding small molecule binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding protein binding protein domain specific binding peptidase activity catalytic activity, acting on a protein threonine-type peptidase activity hydrolase activity catalytic activity endopeptidase activity threonine-type endopeptidase activity identical protein binding	cellular response to stimulus cellular response to heat response to stimulus cellular response to stress response to stress response to heat response to abiotic stimulus cellular process response to temperature stimulus organonitrogen compound catabolic process protein denaturation organonitrogen compound metabolic process catabolic process primary metabolic process cellular response to stimulus cellular response to heat response to stimulus cellular response to stress response to stress response to heat response to abiotic stimulus cellular process response to temperature stimulus organonitrogen compound catabolic process protein denaturation organonitrogen compound metabolic process catabolic process primary metabolic process protein catabolic process macromolecule catabolic process organic substance metabolic process metabolic process protein metabolic process macromolecule metabolic process organic substance catabolic process proteolysis involved in protein catabolic process proteolysis	protein-containing complex intracellular anatomical structure catalytic complex peptidase complex cellular anatomical entity cytosolic proteasome complex HslUV protease complex proteasome complex endopeptidase complex cytoplasm cytosol intracellular protein-containing complex proteasome core complex

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
C [auth E], D [auth F], I [auth G], J [auth H]	IPR003593	AAA+ ATPase domain	Domain
C [auth E], D [auth F], I [auth G], J [auth H]	IPR003959	ATPase, AAA-type, core	Domain
C [auth E], D [auth F], I [auth G], J [auth H]	IPR004491	Heat shock protein HslU	Family
C [auth E], D [auth F], I [auth G], J [auth H]	IPR019489	Clp ATPase, C-terminal	Domain
C [auth E], D [auth F], I [auth G], J [auth H]	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
A, B, E [auth C], F [auth D], G [auth I], A, B, E [auth C], F [auth D], G [auth I], H [auth J], K, L	IPR029055	Nucleophile aminohydrolases, N-terminal	Homologous Superfamily
A, B, E [auth C], F [auth D], G [auth I], A, B, E [auth C], F [auth D], G [auth I], H [auth J], K, L	IPR023333	Proteasome B-type subunit	Family
A, B, E [auth C], F [auth D], G [auth I], A, B, E [auth C], F [auth D], G [auth I], H [auth J], K, L	IPR001353	Proteasome, subunit alpha/beta	Family
A, B, E [auth C], F [auth D], G [auth I], A, B, E [auth C], F [auth D], G [auth I], H [auth J], K, L	IPR022281	ATP-dependent protease, HslV subunit	Family