1H3O

Crystal Structure of the Human TAF4-TAF12 (TAFII135-TAFII20) Complex

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
C	d1h3oc_	All alpha proteins	Histone-fold	Histone-fold	TBP-associated factors, TAFs	TAF(II)-135, (TAF(II)-130, hTAF4), histone fold domain	HUMAN (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
A	d1h3oa_	All alpha proteins	Histone-fold	Histone-fold	TBP-associated factors, TAFs	TAF(II)-135, (TAF(II)-130, hTAF4), histone fold domain	HUMAN (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
D	d1h3od1	All alpha proteins	Histone-fold	Histone-fold	TBP-associated factors, TAFs	TAF(II)-20, (TAF(II)-15, hTAF12), histone fold domain	HUMAN (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
D	d1h3od2	Artifacts	Tags	Tags	Tags	N-terminal Tags	HUMAN (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B	d1h3ob1	All alpha proteins	Histone-fold	Histone-fold	TBP-associated factors, TAFs	TAF(II)-20, (TAF(II)-15, hTAF12), histone fold domain	HUMAN (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B	d1h3ob2	Artifacts	Tags	Tags	Tags	N-terminal Tags	HUMAN (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
C	SCOP2B Superfamily	Core histone-like	8042407	3001387	SCOP2B (2022-06-29)
A	SCOP2 Family	TBP-associated factors TAFs	8030028	4000797	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Core histone-like	8042407	3001387	SCOP2 (2022-06-29)
D	SCOP2B Superfamily	Core histone-like	8042408	3001387	SCOP2B (2022-06-29)
B	SCOP2 Family	TBP-associated factors TAFs	8030029	4000797	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	Core histone-like	8042408	3001387	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
C	TAF4	e1h3oC1	A: alpha arrays	X: Histone-like	H: Histone-related	T: Histone	F: TAF4	ECOD (1.6)
A	TAF4	e1h3oA1	A: alpha arrays	X: Histone-like	H: Histone-related	T: Histone	F: TAF4	ECOD (1.6)
D	TFIID_20kDa	e1h3oD1	A: alpha arrays	X: Histone-like	H: Histone-related	T: Histone	F: TFIID_20kDa	ECOD (1.6)
B	TFIID_20kDa	e1h3oB1	A: alpha arrays	X: Histone-like	H: Histone-related	T: Histone	F: TFIID_20kDa	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
C	1.10.20.10	Mainly Alpha	Orthogonal Bundle	Histone, subunit A	Histone, subunit A	CATH (4.3.0)
A	1.10.20.10	Mainly Alpha	Orthogonal Bundle	Histone, subunit A	Histone, subunit A	CATH (4.3.0)
D	1.10.20.10	Mainly Alpha	Orthogonal Bundle	Histone, subunit A	Histone, subunit A	CATH (4.3.0)
B	1.10.20.10	Mainly Alpha	Orthogonal Bundle	Histone, subunit A	Histone, subunit A	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, C	PF05236	Transcription initiation factor TFIID component TAF4 family (TAF4)	Transcription initiation factor TFIID component TAF4 family	-	Family
B, D	PF03847	Transcription initiation factor TFIID subunit A (TFIID_20kDa)	Transcription initiation factor TFIID subunit A	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, C	TRANSCRIPTION INITIATION FACTOR TFIID 135 KDA SUBUNIT	protein heterodimerization activity protein binding binding protein dimerization activity DNA binding organic cyclic compound binding nucleic acid binding transcription factor binding RNA polymerase II-specific DNA-binding transcription factor binding signaling receptor binding DNA-binding transcription factor binding aryl hydrocarbon receptor binding	cellular biosynthetic process transcription preinitiation complex assembly RNA metabolic process cellular component organization cellular component biogenesis protein-DNA complex organization RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription nucleic acid biosynthetic process cellular component assembly organic substance metabolic process transcription by RNA polymerase II protein-containing complex assembly organic substance biosynthetic process cellular biosynthetic process transcription preinitiation complex assembly RNA metabolic process cellular component organization cellular component biogenesis protein-DNA complex organization RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription nucleic acid biosynthetic process cellular component assembly organic substance metabolic process transcription by RNA polymerase II protein-containing complex assembly organic substance biosynthetic process cellular component organization or biogenesis transcription initiation at RNA polymerase II promoter protein-containing complex organization cellular metabolic process protein-DNA complex assembly primary metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process DNA-templated transcription initiation nucleic acid metabolic process organic cyclic compound metabolic process biosynthetic process organic cyclic compound biosynthetic process RNA polymerase II preinitiation complex assembly cellular process metabolic process macromolecule metabolic process regulation of cellular process positive regulation of macromolecule biosynthetic process regulation of primary metabolic process positive regulation of metabolic process regulation of DNA-templated transcription initiation regulation of RNA metabolic process positive regulation of macromolecule metabolic process regulation of nucleobase-containing compound metabolic process regulation of transcription by RNA polymerase II regulation of biological process positive regulation of nucleobase-containing compound metabolic process regulation of cellular metabolic process positive regulation of transcription by RNA polymerase II positive regulation of DNA-templated transcription initiation positive regulation of RNA metabolic process regulation of gene expression regulation of macromolecule biosynthetic process regulation of DNA-templated transcription regulation of metabolic process positive regulation of DNA-templated transcription positive regulation of biological process positive regulation of cellular metabolic process positive regulation of biosynthetic process positive regulation of cellular process regulation of RNA biosynthetic process regulation of biosynthetic process positive regulation of transcription initiation by RNA polymerase II biological regulation regulation of macromolecule metabolic process regulation of cellular biosynthetic process positive regulation of RNA biosynthetic process regulation of transcription initiation by RNA polymerase II positive regulation of cellular biosynthetic process developmental process multicellular organismal process gonad development reproductive system development reproductive process female gonad development sex differentiation animal organ development female sex differentiation development of primary female sexual characteristics developmental process involved in reproduction multicellular organism development development of primary sexual characteristics anatomical structure development reproductive structure development ovarian follicle development system development mRNA transcription by RNA polymerase II mRNA transcription mRNA metabolic process cellular response to stimulus response to stimulus regulation of cellular response to stress cellular response to stress response to stress regulation of response to stress DNA repair DNA metabolic process regulation of DNA repair DNA damage response regulation of response to stimulus regulation of DNA metabolic process	intracellular anatomical structure cytoplasm cellular anatomical entity cytosol RNA polymerase II transcription regulator complex membrane-bounded organelle RNA polymerase II, holoenzyme protein-containing complex nucleus membrane-enclosed lumen nuclear protein-containing complex transcription regulator complex transferase complex intracellular organelle lumen intracellular anatomical structure cytoplasm cellular anatomical entity cytosol RNA polymerase II transcription regulator complex membrane-bounded organelle RNA polymerase II, holoenzyme protein-containing complex nucleus membrane-enclosed lumen nuclear protein-containing complex transcription regulator complex transferase complex intracellular organelle lumen intracellular organelle nucleoplasm catalytic complex intracellular membrane-bounded organelle transferase complex, transferring phosphorus-containing groups nuclear DNA-directed RNA polymerase complex organelle lumen organelle RNA polymerase complex transcription factor TFIID complex DNA-directed RNA polymerase complex intracellular protein-containing complex nuclear lumen protein-DNA complex intracellular non-membrane-bounded organelle chromatin chromosome non-membrane-bounded organelle transcription factor TFTC complex histone acetyltransferase complex SAGA-type complex acetyltransferase complex protein acetyltransferase complex male germ cell nucleus germ cell nucleus histone methyltransferase complex MLL1/2 complex MLL1 complex methyltransferase complex
B, D	TRANSCRIPTION INITIATION FACTOR TFIID 20/15 KDA SUBUNITS	protein heterodimerization activity protein binding binding protein dimerization activity transcription coactivator activity transcription regulator activity transcription coregulator activity protein-macromolecule adaptor activity molecular adaptor activity DNA binding organic cyclic compound binding nucleic acid binding transcription factor binding TBP-class protein binding protein heterodimerization activity protein binding binding protein dimerization activity transcription coactivator activity transcription regulator activity transcription coregulator activity protein-macromolecule adaptor activity molecular adaptor activity DNA binding organic cyclic compound binding nucleic acid binding transcription factor binding TBP-class protein binding general transcription initiation factor binding DNA-binding transcription factor binding	cellular biosynthetic process transcription preinitiation complex assembly RNA metabolic process cellular component organization cellular component biogenesis protein-DNA complex organization RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription nucleic acid biosynthetic process cellular component assembly organic substance metabolic process transcription by RNA polymerase II protein-containing complex assembly organic substance biosynthetic process cellular biosynthetic process transcription preinitiation complex assembly RNA metabolic process cellular component organization cellular component biogenesis protein-DNA complex organization RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription nucleic acid biosynthetic process cellular component assembly organic substance metabolic process transcription by RNA polymerase II protein-containing complex assembly organic substance biosynthetic process cellular component organization or biogenesis transcription initiation at RNA polymerase II promoter protein-containing complex organization cellular metabolic process protein-DNA complex assembly primary metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process DNA-templated transcription initiation nucleic acid metabolic process organic cyclic compound metabolic process biosynthetic process organic cyclic compound biosynthetic process RNA polymerase II preinitiation complex assembly cellular process metabolic process macromolecule metabolic process regulation of cellular process positive regulation of macromolecule biosynthetic process regulation of primary metabolic process positive regulation of metabolic process regulation of DNA-templated transcription initiation regulation of RNA metabolic process positive regulation of macromolecule metabolic process regulation of nucleobase-containing compound metabolic process regulation of transcription by RNA polymerase II regulation of biological process positive regulation of nucleobase-containing compound metabolic process regulation of cellular metabolic process positive regulation of transcription by RNA polymerase II positive regulation of DNA-templated transcription initiation positive regulation of RNA metabolic process regulation of gene expression regulation of macromolecule biosynthetic process regulation of DNA-templated transcription regulation of metabolic process positive regulation of DNA-templated transcription positive regulation of biological process positive regulation of cellular metabolic process positive regulation of biosynthetic process positive regulation of cellular process regulation of RNA biosynthetic process regulation of biosynthetic process positive regulation of transcription initiation by RNA polymerase II biological regulation regulation of macromolecule metabolic process regulation of cellular biosynthetic process positive regulation of RNA biosynthetic process regulation of transcription initiation by RNA polymerase II positive regulation of cellular biosynthetic process mRNA transcription by RNA polymerase II mRNA transcription mRNA metabolic process cellular response to stimulus response to stimulus regulation of cellular response to stress cellular response to stress response to stress regulation of response to stress DNA repair DNA metabolic process regulation of DNA repair DNA damage response regulation of response to stimulus regulation of DNA metabolic process RNA processing RNA splicing regulation of RNA splicing	RNA polymerase II transcription regulator complex membrane-bounded organelle RNA polymerase II, holoenzyme protein-containing complex intracellular anatomical structure nucleus membrane-enclosed lumen nuclear protein-containing complex transcription regulator complex transferase complex intracellular organelle lumen intracellular organelle nucleoplasm catalytic complex RNA polymerase II transcription regulator complex membrane-bounded organelle RNA polymerase II, holoenzyme protein-containing complex intracellular anatomical structure nucleus membrane-enclosed lumen nuclear protein-containing complex transcription regulator complex transferase complex intracellular organelle lumen intracellular organelle nucleoplasm catalytic complex intracellular membrane-bounded organelle cellular anatomical entity transferase complex, transferring phosphorus-containing groups nuclear DNA-directed RNA polymerase complex organelle lumen organelle RNA polymerase complex transcription factor TFIID complex DNA-directed RNA polymerase complex intracellular protein-containing complex nuclear lumen protein-DNA complex chromatin chromosome histone acetyltransferase complex acetyltransferase complex intracellular non-membrane-bounded organelle protein acetyltransferase complex SAGA-type complex SLIK (SAGA-like) complex non-membrane-bounded organelle peptidase complex SAGA complex transcription factor TFTC complex

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, C	IPR003894	TAFH/NHR1	Domain
A, C	IPR007900	Transcription initiation factor TFIID component TAF4, C-terminal	Domain
A, C	IPR009072	Histone-fold	Homologous Superfamily
A, C	IPR045144	Transcription initiation factor TFIID component TAF4	Family
A, C	IPR037249	TAFH/NHR1 domain superfamily	Homologous Superfamily
B, D	IPR009072	Histone-fold	Homologous Superfamily
B, D	IPR037794	Transcription initiation factor TFIID subunit 12	Family
B, D	IPR003228	Transcription initiation factor TFIID subunit 12 domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, C	Pharos: O00268	lung cancer subependymal giant cell astrocytoma malignant glioma malignant mesothelioma
B, D	Pharos: Q16514	lung adenocarcinoma

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.