Annotations: 1GOT

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1gota1	All alpha proteins	Transducin (alpha subunit), insertion domain	Transducin (alpha subunit), insertion domain	Transducin (alpha subunit), insertion domain	Transducin (alpha subunit), insertion domain	cattle (Bos taurus ) [TaxId: 9913 ],	SCOPe (2.08)
A	d1gota2	Alpha and beta proteins (a/b)	P-loop containing nucleoside triphosphate hydrolases	P-loop containing nucleoside triphosphate hydrolases	G proteins	Transducin (alpha subunit)	cattle (Bos taurus ) [TaxId: 9913 ],	SCOPe (2.08)
B	d1gotb_	All beta proteins	7-bladed beta-propeller	WD40 repeat-like	WD40-repeat	beta1-subunit of the signal-transducing G protein heterotrimer	cattle (Bos taurus ) [TaxId: 9913 ],	SCOPe (2.08)
C [auth G]	d1gotg_	All alpha proteins	Non-globular all-alpha subunits of globular proteins	Transducin (heterotrimeric G protein), gamma chain	Transducin (heterotrimeric G protein), gamma chain	Transducin (heterotrimeric G protein), gamma chain	cattle (Bos taurus ) [TaxId: 9913 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Ras-like P-loop GTPases	8056788	3002022	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Galpha insert domain-like	8056789	3000669	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Ras-like P-loop GTPases	8056788	3002022	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	WD40 repeat-like	8042849	3001694	SCOP2B (2022-06-29)
C [auth G]	SCOP2B Superfamily	Transducin (heterotrimeric G protein) gamma chain	8039083	3001771	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	G-alpha	e1gotA1	A: alpha arrays	X: Transducin (alpha subunit), insertion domain (From Topology)	H: Transducin (alpha subunit), insertion domain (From Topology)	T: Transducin (alpha subunit), insertion domain	F: G-alpha	ECOD (1.6)
A	Ras	e1gotA2	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: Ras	ECOD (1.6)
B	ANAPC4_WD40_3	e1gotB1	A: beta duplicates or obligate multimers	X: beta-propeller-like	H: beta-propeller	T: 7-bladed	F: ANAPC4_WD40_3	ECOD (1.6)
C [auth G]	G-gamma	e1gotG1	A: extended segments	X: Transducin (heterotrimeric G protein), gamma chain (From Topology)	H: Transducin (heterotrimeric G protein), gamma chain (From Topology)	T: Transducin (heterotrimeric G protein), gamma chain	F: G-gamma	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
A	1.10.400.10	Mainly Alpha	Orthogonal Bundle	GI Alpha 1, domain 2-like	GI Alpha 1, domain 2-like	CATH (4.3.0)
B	2.130.10.10	Mainly Beta	7 Propeller	Methylamine Dehydrogenase	Chain H	CATH (4.3.0)
C [auth G]	4.10.260.10	Few Secondary Structures	Irregular	G Protein Gi Gamma 2	Transducin (heterotrimeric G protein), gamma chain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00503	G-protein alpha subunit (G-alpha)	G-protein alpha subunit	G proteins couple receptors of extracellular signals to intracellular signaling pathways. The G protein alpha subunit binds guanyl nucleotide and is a weak GTPase. A set of residues that are unique to G-alpha as compared to its ancestor the Arf-like ...	G proteins couple receptors of extracellular signals to intracellular signaling pathways. The G protein alpha subunit binds guanyl nucleotide and is a weak GTPase. A set of residues that are unique to G-alpha as compared to its ancestor the Arf-like family form a ring of residues centered on the nucleotide binding site [3]. A Ggamma is found fused to an inactive Galpha in the Dictyostelium protein gbqA [3].	Domain
B	PF00400	WD domain, G-beta repeat (WD40)	WD domain, G-beta repeat	-	Repeat
C [auth G]	PF00631	GGL domain (G-gamma)	GGL domain	G-protein gamma like domains (GGL) are found in the gamma subunit of the heterotrimeric G protein complex and in regulators of G protein signaling (RGS) proteins [1]. It is also found fused to an inactive Galpha in the Dictyostelium protein gbqA [2] ...	G-protein gamma like domains (GGL) are found in the gamma subunit of the heterotrimeric G protein complex and in regulators of G protein signaling (RGS) proteins [1]. It is also found fused to an inactive Galpha in the Dictyostelium protein gbqA [2]. G-gamma likely shares a common origin with the helical N-terminal unit of G-beta [2]. All organisms that posses a G-beta possess a G-gamma [2].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	GT-ALPHA/GI-ALPHA CHIMERA	guanyl nucleotide binding binding nucleotide binding GDP binding guanyl ribonucleotide binding purine ribonucleotide binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding guanyl nucleotide binding binding nucleotide binding GDP binding guanyl ribonucleotide binding purine ribonucleotide binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding protein binding kinase binding enzyme binding protein kinase binding protein-containing complex binding G-protein beta/gamma-subunit complex binding signaling receptor binding G protein-coupled receptor binding acyl binding GTP binding purine ribonucleoside triphosphate binding cation binding metal ion binding pyrophosphatase activity GTPase activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity	multicellular organismal process sensory perception of light stimulus system process sensory perception nervous system process visual perception phototransduction cellular response to stimulus detection of light stimulus response to light stimulus detection of external stimulus regulation of biological process regulation of cellular process response to stimulus multicellular organismal process sensory perception of light stimulus system process sensory perception nervous system process visual perception phototransduction cellular response to stimulus detection of light stimulus response to light stimulus detection of external stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation response to external stimulus detection of abiotic stimulus cell communication detection of stimulus cellular process response to abiotic stimulus response to radiation signal transduction negative regulation of hydrolase activity regulation of molecular function regulation of catalytic activity regulation of hydrolase activity negative regulation of cyclic-nucleotide phosphodiesterase activity negative regulation of molecular function negative regulation of catalytic activity regulation of cyclic-nucleotide phosphodiesterase activity adenylate cyclase-modulating G protein-coupled receptor signaling pathway G protein-coupled receptor signaling pathway	membrane-bounded organelle non-motile cilium membrane plasma membrane cilium 9+0 non-motile cilium neuron projection cellular anatomical entity ciliary membrane photoreceptor outer segment membrane photoreceptor outer segment plasma membrane bounded cell projection cell periphery cell projection membrane-bounded organelle non-motile cilium membrane plasma membrane cilium 9+0 non-motile cilium neuron projection cellular anatomical entity ciliary membrane photoreceptor outer segment membrane photoreceptor outer segment plasma membrane bounded cell projection cell periphery cell projection bounding membrane of organelle photoreceptor cell cilium organelle plasma membrane region organelle membrane cell projection membrane protein-containing complex extrinsic component of plasma membrane catalytic complex plasma membrane protein complex cytoplasmic side of membrane membrane protein complex heterotrimeric G-protein complex side of membrane cytoplasmic side of plasma membrane GTPase complex extrinsic component of membrane extrinsic component of cytoplasmic side of plasma membrane photoreceptor inner segment photoreceptor disc membrane plasma membrane signaling receptor complex G protein-coupled receptor complex receptor complex
B	GT-BETA	protein-containing complex binding binding protein binding enzyme binding GTPase binding pyrophosphatase activity GTPase activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity signaling receptor complex adaptor activity protein-macromolecule adaptor activity protein-containing complex binding binding protein binding enzyme binding GTPase binding pyrophosphatase activity GTPase activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity signaling receptor complex adaptor activity protein-macromolecule adaptor activity molecular adaptor activity signaling adaptor activity	cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation phospholipase C-activating G protein-coupled receptor signaling pathway cell communication G protein-coupled receptor signaling pathway cellular process signal transduction multicellular organismal process system process sensory perception of chemical stimulus cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation phospholipase C-activating G protein-coupled receptor signaling pathway cell communication G protein-coupled receptor signaling pathway cellular process signal transduction multicellular organismal process system process sensory perception of chemical stimulus sensory perception nervous system process sensory perception of taste developmental process sensory organ development eye development animal organ development retina development in camera-type eye visual system development multicellular organism development camera-type eye development anatomical structure development sensory system development system development cell population proliferation cellular response to nitrogen compound response to dopamine response to organic substance dopamine receptor signaling pathway cellular response to dopamine response to monoamine cellular response to endogenous stimulus response to catecholamine adenylate cyclase-activating dopamine receptor signaling pathway cellular response to catecholamine stimulus cellular response to organic substance response to organic cyclic compound response to organonitrogen compound response to endogenous stimulus cellular response to organic cyclic compound response to oxygen-containing compound response to nitrogen compound adenylate cyclase-activating G protein-coupled receptor signaling pathway cellular response to oxygen-containing compound cellular response to chemical stimulus cellular response to organonitrogen compound cellular response to monoamine stimulus response to chemical adenylate cyclase-modulating G protein-coupled receptor signaling pathway response to ketone response to prostaglandin response to lipid response to hormone response to prostaglandin E cellular response to ketone cellular response to hormone stimulus response to alcohol cellular response to prostaglandin stimulus cellular response to prostaglandin E stimulus cellular response to alcohol cellular response to lipid	membrane cellular anatomical entity protein-containing complex extrinsic component of plasma membrane catalytic complex plasma membrane protein complex cytoplasmic side of membrane plasma membrane membrane protein complex heterotrimeric G-protein complex side of membrane cell periphery cytoplasmic side of plasma membrane GTPase complex membrane cellular anatomical entity protein-containing complex extrinsic component of plasma membrane catalytic complex plasma membrane protein complex cytoplasmic side of membrane plasma membrane membrane protein complex heterotrimeric G-protein complex side of membrane cell periphery cytoplasmic side of plasma membrane GTPase complex extrinsic component of membrane extrinsic component of cytoplasmic side of plasma membrane membrane-bounded organelle non-motile cilium 9+0 non-motile cilium cilium neuron projection photoreceptor disc membrane photoreceptor outer segment plasma membrane bounded cell projection cell projection photoreceptor cell cilium organelle organelle membrane intracellular anatomical structure cytoplasm
C [auth G]	GT-GAMMA	protein binding binding G-protein beta-subunit binding	cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation G protein-coupled receptor signaling pathway cellular process signal transduction cell communication protein localization localization cellular localization cellular macromolecule localization cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation G protein-coupled receptor signaling pathway cellular process signal transduction cell communication protein localization localization cellular localization cellular macromolecule localization macromolecule localization multicellular organismal process cellular developmental process sensory organ development photoreceptor cell differentiation visual system development cell development eye morphogenesis multicellular organism development neurogenesis neuron differentiation cell differentiation eye photoreceptor cell differentiation anatomical structure development animal organ morphogenesis sensory system development system development photoreceptor cell development developmental process eye photoreceptor cell development eye development animal organ development nervous system development generation of neurons anatomical structure morphogenesis sensory organ morphogenesis neuron development phototransduction detection of light stimulus response to light stimulus detection of external stimulus response to external stimulus detection of abiotic stimulus detection of stimulus response to abiotic stimulus response to radiation	protein-containing complex extrinsic component of plasma membrane catalytic complex plasma membrane protein complex cytoplasmic side of membrane membrane plasma membrane membrane protein complex cellular anatomical entity heterotrimeric G-protein complex side of membrane cell periphery cytoplasmic side of plasma membrane GTPase complex protein-containing complex extrinsic component of plasma membrane catalytic complex plasma membrane protein complex cytoplasmic side of membrane membrane plasma membrane membrane protein complex cellular anatomical entity heterotrimeric G-protein complex side of membrane cell periphery cytoplasmic side of plasma membrane GTPase complex extrinsic component of membrane extrinsic component of cytoplasmic side of plasma membrane membrane-bounded organelle non-motile cilium 9+0 non-motile cilium cilium neuron projection photoreceptor disc membrane photoreceptor outer segment plasma membrane bounded cell projection cell projection photoreceptor cell cilium organelle organelle membrane

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR001408	G-protein alpha subunit, group I	Family
A	IPR001019	Guanine nucleotide binding protein (G-protein), alpha subunit	Family
A	IPR011025	G protein alpha subunit, helical insertion	Homologous Superfamily
A	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
B	IPR019775	WD40 repeat, conserved site	Conserved Site
B	IPR016346	Guanine nucleotide-binding protein, beta subunit	Family
B	IPR036322	WD40-repeat-containing domain superfamily	Homologous Superfamily
B	IPR001632	G-protein, beta subunit	Domain
B	IPR001680	WD40 repeat	Repeat
B	IPR020472	G-protein beta WD-40 repeat	Repeat
B	IPR015943	WD40/YVTN repeat-like-containing domain superfamily	Homologous Superfamily
C [auth G]	IPR036284	G-protein gamma-like domain superfamily	Homologous Superfamily
C [auth G]	IPR015898	G-protein gamma-like domain	Domain
C [auth G]	IPR001770	G-protein, gamma subunit	Family

Membrane Protein Annotation: OPM OPM Database Homepage

Chains	External Link	Type	Class	Superfamily	Family
A, C [auth G]	OPM	Monotopic/peripheral	Alpha/Beta monotopic/peripheral	P-loop containing nucleoside triphosphate hydrolases	G-proteins

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.