1EYI

FRUCTOSE-1,6-BISPHOSPHATASE COMPLEX WITH MAGNESIUM, FRUCTOSE-6-PHOSPHATE AND PHOSPHATE (R-STATE)

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Structure Motif: Primary M-CSA Annotation

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1eyia_	Multi-domain proteins (alpha and beta)	Carbohydrate phosphatase	Carbohydrate phosphatase	Inositol monophosphatase/fructose-1,6-bisphosphatase-like	Fructose-1,6-bisphosphatase	pig (Sus scrofa ) [TaxId: 9823 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Carbohydrate phosphatase-like	8033633	3001721	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF00316	e1eyiA1	A: a+b two layers	X: a+b domain in carbohydrate phosphatases (From Topology)	H: a+b domain in carbohydrate phosphatases (From Topology)	T: a+b domain in carbohydrate phosphatases	F: PF00316	ECOD (1.6)
A	PF18913	e1eyiA2	A: a/b three-layered sandwiches	X: Rossmann-like domain in carbohydrate phosphatases (From Homology)	H: Rossmann-like domain in carbohydrate phosphatases	T: Rossmann-like domain in carbohydrate phosphatases	F: PF18913	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.30.540.10	Alpha Beta	2-Layer Sandwich	Fructose-1,6-Bisphosphatase	Chain A, domain 1	CATH (4.3.0)
A	3.40.190.80	Alpha Beta	3-Layer(aba) Sandwich	D-Maltodextrin-Binding Protein	domain 2	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00316	Fructose-1-6-bisphosphatase, N-terminal domain (FBPase)	Fructose-1-6-bisphosphatase, N-terminal domain	This family represents the N-terminus of this protein family.	Domain
A	PF18913	Fructose-1-6-bisphosphatase, C-terminal domain (FBPase_C)	Fructose-1-6-bisphosphatase, C-terminal domain	This entry represents the C-terminal domain of Fructose-1-6-bisphosphatase enzymes. According to ECOD this domain has a Rossmann-like fold.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	FRUCTOSE-1,6-BISPHOSPHATASE	phosphoric ester hydrolase activity fructose 1,6-bisphosphate 1-phosphatase activity hydrolase activity, acting on ester bonds carbohydrate phosphatase activity phosphatase activity hydrolase activity catalytic activity sugar-phosphatase activity transcription factor binding RNA polymerase II-specific DNA-binding transcription factor binding protein binding binding DNA-binding transcription factor binding adenyl ribonucleotide binding phosphoric ester hydrolase activity fructose 1,6-bisphosphate 1-phosphatase activity hydrolase activity, acting on ester bonds carbohydrate phosphatase activity phosphatase activity hydrolase activity catalytic activity sugar-phosphatase activity transcription factor binding RNA polymerase II-specific DNA-binding transcription factor binding protein binding binding DNA-binding transcription factor binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding cation binding AMP binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding monosaccharide binding carbohydrate binding metal ion binding identical protein binding	organic substance metabolic process organophosphate metabolic process cellular metabolic process phosphate-containing compound metabolic process fructose 6-phosphate metabolic process carbohydrate derivative metabolic process metabolic process cellular process phosphorus metabolic process monosaccharide metabolic process gluconeogenesis regulation of primary metabolic process regulation of small molecule metabolic process organic substance biosynthetic process organic substance metabolic process organophosphate metabolic process cellular metabolic process phosphate-containing compound metabolic process fructose 6-phosphate metabolic process carbohydrate derivative metabolic process metabolic process cellular process phosphorus metabolic process monosaccharide metabolic process gluconeogenesis regulation of primary metabolic process regulation of small molecule metabolic process organic substance biosynthetic process small molecule metabolic process regulation of carbohydrate metabolic process regulation of glucose metabolic process regulation of biosynthetic process carbohydrate metabolic process regulation of biological process small molecule biosynthetic process carbohydrate biosynthetic process biological regulation hexose metabolic process primary metabolic process monosaccharide biosynthetic process hexose biosynthetic process biosynthetic process regulation of gluconeogenesis regulation of carbohydrate biosynthetic process regulation of metabolic process glucose metabolic process fructose metabolic process pyridine nucleotide catabolic process oxoacid metabolic process carbohydrate catabolic process organophosphate catabolic process regulation of generation of precursor metabolites and energy nucleoside phosphate catabolic process organic acid metabolic process regulation of ATP metabolic process regulation of purine nucleotide catabolic process negative regulation of nucleotide metabolic process regulation of nucleotide metabolic process regulation of phosphate metabolic process purine ribonucleotide catabolic process negative regulation of carbohydrate metabolic process nucleoside phosphate metabolic process organonitrogen compound catabolic process pyruvate metabolic process negative regulation of metabolic process negative regulation of purine nucleotide catabolic process purine nucleoside diphosphate metabolic process purine-containing compound catabolic process regulation of purine nucleotide metabolic process nucleobase-containing compound catabolic process nucleoside diphosphate metabolic process regulation of phosphorus metabolic process monocarboxylic acid metabolic process pyridine-containing compound catabolic process ribose phosphate metabolic process ATP metabolic process negative regulation of purine nucleotide metabolic process negative regulation of phosphate metabolic process nucleobase-containing small molecule metabolic process purine ribonucleoside triphosphate metabolic process negative regulation of nucleotide catabolic process purine nucleotide catabolic process carbohydrate derivative catabolic process organic cyclic compound catabolic process purine-containing compound metabolic process ADP metabolic process negative regulation of phosphorus metabolic process purine ribonucleoside diphosphate metabolic process organic substance catabolic process purine ribonucleoside diphosphate catabolic process ribonucleotide catabolic process regulation of cellular process negative regulation of small molecule metabolic process negative regulation of glycolytic process negative regulation of biological process negative regulation of ATP metabolic process negative regulation of cellular metabolic process nucleobase-containing compound metabolic process glycolytic process pyridine-containing compound metabolic process negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process pyridine nucleotide metabolic process nicotinamide nucleotide metabolic process regulation of carbohydrate catabolic process regulation of cellular metabolic process nucleotide metabolic process organic cyclic compound metabolic process purine nucleoside diphosphate catabolic process nucleoside diphosphate catabolic process regulation of nucleotide catabolic process nucleoside triphosphate metabolic process purine ribonucleotide metabolic process catabolic process purine nucleotide metabolic process ribonucleotide metabolic process negative regulation of cellular process ribonucleoside diphosphate metabolic process ribonucleoside diphosphate catabolic process nucleotide catabolic process ribonucleoside triphosphate metabolic process negative regulation of catabolic process purine nucleoside triphosphate metabolic process organonitrogen compound metabolic process ADP catabolic process carboxylic acid metabolic process regulation of catabolic process generation of precursor metabolites and energy regulation of glycolytic process regulation of growth regulation of cellular component organization growth negative regulation of cell growth regulation of cell growth cell growth cellular component organization negative regulation of growth cellular component organization or biogenesis cellular response to stimulus small GTPase-mediated signal transduction response to stimulus signaling negative regulation of signaling regulation of small GTPase mediated signal transduction negative regulation of intracellular signal transduction regulation of signal transduction intracellular signal transduction negative regulation of cell communication cell communication regulation of intracellular signal transduction regulation of Ras protein signal transduction intracellular signaling cassette signal transduction regulation of signaling negative regulation of response to stimulus Ras protein signal transduction negative regulation of small GTPase mediated signal transduction negative regulation of signal transduction negative regulation of Ras protein signal transduction regulation of response to stimulus regulation of cell communication sucrose biosynthetic process oligosaccharide metabolic process disaccharide metabolic process disaccharide biosynthetic process sucrose metabolic process oligosaccharide biosynthetic process cellular response to inorganic substance cellular response to metal ion response to inorganic substance cellular response to chemical stimulus response to metal ion response to chemical response to magnesium ion cellular response to magnesium ion fructose 1,6-bisphosphate metabolic process cellular response to xenobiotic stimulus response to xenobiotic stimulus negative regulation of RNA biosynthetic process DNA-templated transcription regulation of RNA metabolic process transcription by RNA polymerase II regulation of transcription by RNA polymerase II negative regulation of DNA-templated transcription negative regulation of transcription by RNA polymerase II negative regulation of cellular biosynthetic process regulation of gene expression negative regulation of macromolecule biosynthetic process organic cyclic compound biosynthetic process regulation of macromolecule biosynthetic process macromolecule metabolic process regulation of DNA-templated transcription negative regulation of RNA metabolic process cellular biosynthetic process RNA metabolic process RNA biosynthetic process nucleic acid biosynthetic process regulation of RNA biosynthetic process negative regulation of macromolecule metabolic process regulation of macromolecule metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process regulation of cellular biosynthetic process negative regulation of biosynthetic process dephosphorylation	intracellular anatomical structure cytoplasm cellular anatomical entity cytosol membrane-bounded organelle intracellular organelle organelle nucleus intracellular membrane-bounded organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR020548	Fructose-1,6-bisphosphatase, active site	Active Site
A	IPR033391	Fructose-1-6-bisphosphatase class I, N-terminal	Domain
A	IPR000146	Fructose-1,6-bisphosphatase class 1	Family
A	IPR028343	Fructose-1,6-bisphosphatase	Family
A	IPR044015	Fructose-1-6-bisphosphatase class 1, C-terminal	Domain

Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage

Chains	Enzyme Name	Description	Catalytic Residues
A	fructose-bisphosphatase M-CSA #546	Fructose 1,6-bisphosphatase (FBPase) hydrolyses fructose 1,6-bis-phosphate to fructose 6-phosphate and phosphate. The enzyme plays a crucial role in gluconeogenesis, the formation of glucose from non-carbohydrate carbon containing substrates. The enzyme is only capable of catalysing the forward reaction, while phosphofructokinase catalyses the reverse reaction. Regulation of the two enzyme is performed by metabolites such as fructose 2,6 bis-phosphate, ensuring that enhances reactivity of one enzyme is accompanied by suppressed reactivity of the other.	Defined by 8 residues: ASP:A-68ASP:A-74GLU:A-97GLU:A-98ASP:A-118LEU:A-120ASP:A-121GLU:A-280 \| Explore in 3D: M-CSA Motif Definition Search M-CSA Motif EC: 3.1.3.11 (PDB Primary Data) Search M-CSA Motif + EC 3.1.3.11

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.