Annotations: 1EH6

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1eh6a1	All alpha proteins	DNA/RNA-binding 3-helical bundle	Methylated DNA-protein cysteine methyltransferase, C-terminal domain	Methylated DNA-protein cysteine methyltransferase, C-terminal domain	O6-alkylguanine-DNA alkyltransferase	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
A	d1eh6a2	Alpha and beta proteins (a/b)	Ribonuclease H-like motif	Methylated DNA-protein cysteine methyltransferase domain	Methylated DNA-protein cysteine methyltransferase domain	O6-alkylguanine-DNA alkyltransferase	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Methylated DNA-protein cysteine methyltransferase domain	8044559	3000599	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Methylated DNA-protein cysteine methyltransferase C-terminal domain	8036537	3001016	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	DNA_binding_1	e1eh6A1	A: alpha arrays	X: HTH	H: HTH	T: Methylated DNA-protein cysteine methyltransferase-C	F: DNA_binding_1	ECOD (1.6)
A	Methyltransf_1N	e1eh6A2	A: mixed a+b and a/b	X: Ribonuclease H-like	H: Methylated DNA-protein cysteine methyltransferase domain (From Topology)	T: Methylated DNA-protein cysteine methyltransferase domain	F: Methyltransf_1N	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.30.160.70	Alpha Beta	2-Layer Sandwich	Double Stranded RNA Binding Domain	Methylated DNA-protein cysteine methyltransferase domain	CATH (4.3.0)
A	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF02870	6-O-methylguanine DNA methyltransferase, ribonuclease-like domain (Methyltransf_1N)	6-O-methylguanine DNA methyltransferase, ribonuclease-like domain	This entry represents the N-terminal ribonuclease-like domain associated with 6-O-methylguanine DNA methyltransferase activity. The repair of DNA containing O6-alkylated guanine is carried out by DNA-[protein]-cysteine S-methyltransferase (also known ...	This entry represents the N-terminal ribonuclease-like domain associated with 6-O-methylguanine DNA methyltransferase activity. The repair of DNA containing O6-alkylated guanine is carried out by DNA-[protein]-cysteine S-methyltransferase (also known as O-6-methylguanine-DNA-alkyltransferase)	Domain
A	PF01035	6-O-methylguanine DNA methyltransferase, DNA binding domain (DNA_binding_1)	6-O-methylguanine DNA methyltransferase, DNA binding domain	This is the C-terminal DNA-binding domain of 6-O-methylguanine-DNA methyltransferases [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	O6-ALKYLGUANINE-DNA ALKYLTRANSFERASE	DNA binding binding organic cyclic compound binding nucleic acid binding transferase activity, transferring one-carbon groups transferase activity methyltransferase activity catalytic activity catalytic activity, acting on a protein S-methyltransferase activity protein methyltransferase activity methylated-DNA-[protein]-cysteine S-methyltransferase activity DNA-methyltransferase activity catalytic activity, acting on DNA DNA binding binding organic cyclic compound binding nucleic acid binding transferase activity, transferring one-carbon groups transferase activity methyltransferase activity catalytic activity catalytic activity, acting on a protein S-methyltransferase activity protein methyltransferase activity methylated-DNA-[protein]-cysteine S-methyltransferase activity DNA-methyltransferase activity catalytic activity, acting on DNA catalytic activity, acting on a nucleic acid cation binding ion binding metal ion binding small molecule binding	cellular response to stimulus regulation of cellular process response to stimulus regulation of double-strand break repair positive regulation of biological process regulation of cellular response to stress regulation of primary metabolic process positive regulation of response to stimulus nucleobase-containing compound metabolic process positive regulation of metabolic process positive regulation of DNA repair organic substance metabolic process cellular response to stress response to stress regulation of response to stress cellular response to stimulus regulation of cellular process response to stimulus regulation of double-strand break repair positive regulation of biological process regulation of cellular response to stress regulation of primary metabolic process positive regulation of response to stimulus nucleobase-containing compound metabolic process positive regulation of metabolic process positive regulation of DNA repair organic substance metabolic process cellular response to stress response to stress regulation of response to stress positive regulation of macromolecule metabolic process regulation of nucleobase-containing compound metabolic process positive regulation of cellular process DNA repair double-strand break repair regulation of biological process positive regulation of nucleobase-containing compound metabolic process biological regulation primary metabolic process DNA metabolic process regulation of macromolecule metabolic process positive regulation of double-strand break repair nucleic acid metabolic process regulation of DNA repair organic cyclic compound metabolic process positive regulation of DNA metabolic process regulation of response to stimulus DNA damage response metabolic process cellular process macromolecule metabolic process regulation of metabolic process regulation of DNA metabolic process negative regulation of programmed cell death regulation of apoptotic process negative regulation of biological process regulation of programmed cell death cell death negative regulation of apoptotic process negative regulation of cellular process apoptotic process programmed cell death DNA alkylation repair DNA ligation	membrane cellular anatomical entity membrane-bounded organelle intracellular anatomical structure nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen intracellular organelle nucleoplasm organelle lumen organelle nuclear lumen

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR008332	Methylguanine DNA methyltransferase, ribonuclease-like domain	Domain
A	IPR036217	Methylated DNA-protein cysteine methyltransferase, DNA binding domain	Homologous Superfamily
A	IPR036388	Winged helix-like DNA-binding domain superfamily	Homologous Superfamily
A	IPR036631	Methylated DNA-protein cysteine methyltransferase domain superfamily	Homologous Superfamily
A	IPR014048	Methylated-DNA-[protein]-cysteine S-methyltransferase, DNA binding	Domain
A	IPR001497	Methylated-DNA-[protein]-cysteine S-methyltransferase, active site	Active Site

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A	Pharos: P16455	malignant glioma giant cell glioblastoma esophageal squamous cell carcinoma glioblastoma Necrosis gliosarcoma mixed glioma familial melanoma leukemia malignant mesothelioma oral cavity neoplasm anorexia nervosa peripheral neuropathy bone disease spinal cord disease malignant glioma giant cell glioblastoma esophageal squamous cell carcinoma glioblastoma Necrosis gliosarcoma mixed glioma familial melanoma leukemia malignant mesothelioma oral cavity neoplasm anorexia nervosa peripheral neuropathy bone disease spinal cord disease osteosarcoma astrocytic tumor precancerous condition brain cancer head and neck squamous cell carcinoma oligodendroglioma peripheral nervous system disease brain disease cancer tuberculosis glioma

Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage

Chains	Enzyme Name	Description	Catalytic Residues
A	methylated-DNA--[protein]-cysteine S-methyltransferase M-CSA #251	Human O6-alkylguanine-DNA alkyltransferase (AGT) acts to repair cytotoxic lesions in DNA by transfering the O6-alkyl groups to an active site cysteine. This mechanism for preserving genomic integrity limits the effectiveness of certain alkylating anticancer reagents, and therefore AGT is an active anticancer drug target. This enzyme catalyses only one turnover and therefore is not strictly catalytic.	Defined by 5 residues: TYR:A-114ASN:A-137CYS:A-145HIS:A-146GLU:A-172 \| Explore in 3D: M-CSA Motif Definition Search M-CSA Motif EC: 2.1.1.63 (PDB Primary Data) Search M-CSA Motif + EC 2.1.1.63

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.