1E9R

Bacterial conjugative coupling protein TrwBdeltaN70. Trigonal form in complex with sulphate.

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
B	d1e9rb_	Alpha and beta proteins (a/b)	P-loop containing nucleoside triphosphate hydrolases	P-loop containing nucleoside triphosphate hydrolases	RecA protein-like (ATPase-domain)	Bacterial conjugative coupling protein TrwB	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
E [auth F]	d1e9rf_	Alpha and beta proteins (a/b)	P-loop containing nucleoside triphosphate hydrolases	P-loop containing nucleoside triphosphate hydrolases	RecA protein-like (ATPase-domain)	Bacterial conjugative coupling protein TrwB	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
A	d1e9ra_	Alpha and beta proteins (a/b)	P-loop containing nucleoside triphosphate hydrolases	P-loop containing nucleoside triphosphate hydrolases	RecA protein-like (ATPase-domain)	Bacterial conjugative coupling protein TrwB	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
C [auth D]	d1e9rd_	Alpha and beta proteins (a/b)	P-loop containing nucleoside triphosphate hydrolases	P-loop containing nucleoside triphosphate hydrolases	RecA protein-like (ATPase-domain)	Bacterial conjugative coupling protein TrwB	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
D [auth E]	d1e9re_	Alpha and beta proteins (a/b)	P-loop containing nucleoside triphosphate hydrolases	P-loop containing nucleoside triphosphate hydrolases	RecA protein-like (ATPase-domain)	Bacterial conjugative coupling protein TrwB	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
F [auth G]	d1e9rg_	Alpha and beta proteins (a/b)	P-loop containing nucleoside triphosphate hydrolases	P-loop containing nucleoside triphosphate hydrolases	RecA protein-like (ATPase-domain)	Bacterial conjugative coupling protein TrwB	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
B	SCOP2 Family	TrwB-like	8018215	4004009	SCOP2 (2022-06-29)
B	SCOP2 Family	TrwB insert domain-like	8091678	4007754	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	RecA-like P-loop NTPases	8018216	3002019	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	TrwB/VirB4 insert domain-like	8056658	3002354	SCOP2 (2022-06-29)
E [auth F]	SCOP2B Superfamily	TrwB/VirB4 insert domain-like	8056658	3002354	SCOP2B (2022-06-29)
E [auth F]	SCOP2B Superfamily	RecA-like P-loop NTPases	8018216	3002019	SCOP2B (2022-06-29)
E [auth F]	SCOP2B Superfamily	RecA-like P-loop NTPases	8018216	3002019	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	RecA-like P-loop NTPases	8018216	3002019	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	RecA-like P-loop NTPases	8018216	3002019	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	TrwB/VirB4 insert domain-like	8056658	3002354	SCOP2B (2022-06-29)
C [auth D]	SCOP2B Superfamily	RecA-like P-loop NTPases	8018216	3002019	SCOP2B (2022-06-29)
C [auth D]	SCOP2B Superfamily	TrwB/VirB4 insert domain-like	8056658	3002354	SCOP2B (2022-06-29)
C [auth D]	SCOP2B Superfamily	RecA-like P-loop NTPases	8018216	3002019	SCOP2B (2022-06-29)
D [auth E]	SCOP2B Superfamily	RecA-like P-loop NTPases	8018216	3002019	SCOP2B (2022-06-29)
D [auth E]	SCOP2B Superfamily	RecA-like P-loop NTPases	8018216	3002019	SCOP2B (2022-06-29)
D [auth E]	SCOP2B Superfamily	TrwB/VirB4 insert domain-like	8056658	3002354	SCOP2B (2022-06-29)
F [auth G]	SCOP2B Superfamily	TrwB/VirB4 insert domain-like	8056658	3002354	SCOP2B (2022-06-29)
F [auth G]	SCOP2B Superfamily	RecA-like P-loop NTPases	8018216	3002019	SCOP2B (2022-06-29)
F [auth G]	SCOP2B Superfamily	RecA-like P-loop NTPases	8018216	3002019	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
B	TrwB_AAD_bind	e1e9rB1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: TrwB_AAD_bind	ECOD (1.6)
E [auth F]	TrwB_AAD_bind	e1e9rF1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: TrwB_AAD_bind	ECOD (1.6)
A	TrwB_AAD_bind	e1e9rA1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: TrwB_AAD_bind	ECOD (1.6)
C [auth D]	TrwB_AAD_bind	e1e9rD1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: TrwB_AAD_bind	ECOD (1.6)
D [auth E]	TrwB_AAD_bind	e1e9rE1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: TrwB_AAD_bind	ECOD (1.6)
F [auth G]	TrwB_AAD_bind	e1e9rG1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: TrwB_AAD_bind	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
B	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
B	1.10.8.80	Mainly Alpha	Orthogonal Bundle	Helicase, Ruva Protein	domain 3	CATH (4.3.0)
E [auth F]	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
E [auth F]	1.10.8.80	Mainly Alpha	Orthogonal Bundle	Helicase, Ruva Protein	domain 3	CATH (4.3.0)
A	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
A	1.10.8.80	Mainly Alpha	Orthogonal Bundle	Helicase, Ruva Protein	domain 3	CATH (4.3.0)
C [auth D]	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
C [auth D]	1.10.8.80	Mainly Alpha	Orthogonal Bundle	Helicase, Ruva Protein	domain 3	CATH (4.3.0)
D [auth E]	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
D [auth E]	1.10.8.80	Mainly Alpha	Orthogonal Bundle	Helicase, Ruva Protein	domain 3	CATH (4.3.0)
F [auth G]	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
F [auth G]	1.10.8.80	Mainly Alpha	Orthogonal Bundle	Helicase, Ruva Protein	domain 3	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C [auth D], D [auth E], E [auth F], A, B, C [auth D], D [auth E], E [auth F], F [auth G]	PF10412	Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)	Type IV secretion-system coupling protein DNA-binding domain	The plasmid conjugative coupling protein TrwB forms hexamers from six structurally very similar protomers [1]. This hexamer contains a central channel running from the cytosolic pole (made up by the AADs) to the membrane pole ending at the transmembr ...	The plasmid conjugative coupling protein TrwB forms hexamers from six structurally very similar protomers [1]. This hexamer contains a central channel running from the cytosolic pole (made up by the AADs) to the membrane pole ending at the transmembrane pore shaped by 12 transmembrane helices, rendering an overall mushroom-like structure. The TrwB_AAD (all-alpha domain) domain appears to be the DNA-binding domain of the structure. TrwB, a basic integral inner-membrane nucleoside-triphosphate-binding protein, is the structural prototype for the type IV secretion system coupling proteins, a family of proteins essential for macromolecular transport between cells and export [2].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C [auth D], D [auth E], E [auth F], A, B, C [auth D], D [auth E], E [auth F], F [auth G]	CONJUGAL TRANSFER PROTEIN TRWB	binding nucleoside phosphate binding organic cyclic compound binding nucleotide binding heterocyclic compound binding small molecule binding identical protein binding protein binding	-	membrane cellular anatomical entity

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C [auth D], D [auth E], E [auth F], A, B, C [auth D], D [auth E], E [auth F], F [auth G]	IPR019476	Type IV secretion system coupling protein TraD, DNA-binding domain	Domain
A, B, C [auth D], D [auth E], E [auth F], A, B, C [auth D], D [auth E], E [auth F], F [auth G]	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.