1DGK

MUTANT MONOMER OF RECOMBINANT HUMAN HEXOKINASE TYPE I WITH GLUCOSE AND ADP IN THE ACTIVE SITE

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations
Structure Motif: Primary M-CSA Annotation

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A [auth N]	d1dgkn1	Alpha and beta proteins (a/b)	Ribonuclease H-like motif	Actin-like ATPase domain	Hexokinase	Mammalian type I hexokinase	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A [auth N]	SCOP2B Superfamily	Actin-like ATPases	8044491	3000092	SCOP2B (2022-06-29)
A [auth N]	SCOP2B Superfamily	Actin-like ATPases	8044492	3000092	SCOP2B (2022-06-29)
A [auth N]	SCOP2B Superfamily	Actin-like ATPases	8044493	3000092	SCOP2B (2022-06-29)
A [auth N]	SCOP2B Superfamily	Actin-like ATPases	8044495	3000092	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A [auth N]	Hexokinase_1	e1dgkN1	A: mixed a+b and a/b	X: Ribonuclease H-like	H: Ribonuclease H-like (From Topology)	T: Ribonuclease H-like	F: Hexokinase_1	ECOD (1.6)
A [auth N]	Hexokinase_2	e1dgkN3	A: mixed a+b and a/b	X: Ribonuclease H-like	H: Ribonuclease H-like (From Topology)	T: Ribonuclease H-like	F: Hexokinase_2	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A [auth N]	3.30.420.40	Alpha Beta	2-Layer Sandwich	Nucleotidyltransferase	domain 5	CATH (4.3.0)
A [auth N]	3.40.367.20	Alpha Beta	3-Layer(aba) Sandwich	Hexokinase	domain 1	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A [auth N]	PF03727	Hexokinase (Hexokinase_2)	Hexokinase	Hexokinase (EC:2.7.1.1) contains two structurally similar domains represented by this family and Pfam:PF00349. Some members of the family have two copies of each of these domains.	Domain
A [auth N]	PF00349	Hexokinase (Hexokinase_1)	Hexokinase	Hexokinase (EC:2.7.1.1) contains two structurally similar domains represented by this family and Pfam:PF03727. Some members of the family have two copies of each of these domains.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth N]	HEXOKINASE TYPE I	kinase activity glucosamine kinase activity transferase activity catalytic activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups glycosaminoglycan binding binding carbohydrate derivative binding peptidoglycan binding carbohydrate kinase activity hexokinase activity glucokinase activity adenyl ribonucleotide binding kinase activity glucosamine kinase activity transferase activity catalytic activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups glycosaminoglycan binding binding carbohydrate derivative binding peptidoglycan binding carbohydrate kinase activity hexokinase activity glucokinase activity adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding ribonucleotide binding small molecule binding fructokinase activity glucose binding monosaccharide binding carbohydrate binding mannokinase activity	organic substance metabolic process organophosphate metabolic process cellular metabolic process phosphate-containing compound metabolic process fructose 6-phosphate metabolic process carbohydrate derivative metabolic process metabolic process cellular process phosphorus metabolic process cellular homeostasis chemical homeostasis homeostatic process glucose homeostasis carbohydrate homeostasis organic substance metabolic process organophosphate metabolic process cellular metabolic process phosphate-containing compound metabolic process fructose 6-phosphate metabolic process carbohydrate derivative metabolic process metabolic process cellular process phosphorus metabolic process cellular homeostasis chemical homeostasis homeostatic process glucose homeostasis carbohydrate homeostasis intracellular chemical homeostasis intracellular glucose homeostasis glucose 6-phosphate metabolic process biological process involved in interspecies interaction between organisms response to stimulus immune response response to other organism defense response defense response to symbiont response to external stimulus response to biotic stimulus immune system process defense response to other organism response to stress innate immune response response to external biotic stimulus carbohydrate phosphorylation carbohydrate metabolic process phosphorylation primary metabolic process multicellular organismal process regulation of cellular process interleukin-1 production cellular biosynthetic process positive regulation of biological process positive regulation of cellular metabolic process regulation of interleukin-1 beta production positive regulation of macromolecule biosynthetic process positive regulation of metabolic process positive regulation of interleukin-1 beta production positive regulation of biosynthetic process regulation of cytokine production organic substance biosynthetic process cytokine production regulation of multicellular organismal process positive regulation of interleukin-1 production positive regulation of macromolecule metabolic process positive regulation of cellular process regulation of biosynthetic process regulation of biological process regulation of interleukin-1 production biological regulation regulation of cellular metabolic process regulation of macromolecule metabolic process gene expression macromolecule biosynthetic process regulation of cellular biosynthetic process regulation of gene expression biosynthetic process interleukin-1 beta production positive regulation of gene expression positive regulation of multicellular organismal process regulation of macromolecule biosynthetic process macromolecule metabolic process positive regulation of cytokine production regulation of metabolic process positive regulation of cellular biosynthetic process monosaccharide metabolic process hexose metabolic process small molecule metabolic process glucose metabolic process protein localization establishment of protein localization cellular localization establishment of localization localization establishment of protein localization to organelle cellular macromolecule localization macromolecule localization protein localization to organelle protein localization to mitochondrion establishment of protein localization to mitochondrion ribonucleotide catabolic process pyridine nucleotide catabolic process oxoacid metabolic process carbohydrate catabolic process organophosphate catabolic process nucleoside phosphate catabolic process organic acid metabolic process nucleobase-containing compound metabolic process glycolytic process pyridine-containing compound metabolic process purine ribonucleotide catabolic process pyridine nucleotide metabolic process nucleoside phosphate metabolic process organonitrogen compound catabolic process nicotinamide nucleotide metabolic process pyruvate metabolic process purine nucleoside diphosphate metabolic process purine-containing compound catabolic process nucleotide metabolic process nucleobase-containing compound catabolic process organic cyclic compound metabolic process nucleoside diphosphate metabolic process purine nucleoside diphosphate catabolic process nucleoside diphosphate catabolic process nucleoside triphosphate metabolic process monocarboxylic acid metabolic process purine ribonucleotide metabolic process pyridine-containing compound catabolic process ribose phosphate metabolic process catabolic process ATP metabolic process purine nucleotide metabolic process ribonucleotide metabolic process nucleobase-containing small molecule metabolic process purine ribonucleoside triphosphate metabolic process ribonucleoside diphosphate metabolic process ribonucleoside diphosphate catabolic process nucleotide catabolic process ribonucleoside triphosphate metabolic process purine nucleoside triphosphate metabolic process purine nucleotide catabolic process carbohydrate derivative catabolic process organonitrogen compound metabolic process ADP catabolic process carboxylic acid metabolic process generation of precursor metabolites and energy organic cyclic compound catabolic process purine-containing compound metabolic process ADP metabolic process purine ribonucleoside diphosphate metabolic process organic substance catabolic process purine ribonucleoside diphosphate catabolic process mannose metabolic process inflammatory response NADH regeneration glucose catabolic process to pyruvate hexose catabolic process NADH metabolic process glucose catabolic process monosaccharide catabolic process small molecule catabolic process canonical glycolysis NAD metabolic process glycolytic process through glucose-6-phosphate NAD catabolic process glycolytic process through fructose-6-phosphate maintenance of protein location maintenance of protein location in mitochondrion maintenance of location maintenance of location in cell maintenance of protein location in cell maintenance of protein localization in organelle regulation of immune effector process regulation of immune system process positive regulation of immune system process regulation of cytokine production involved in immune response immune effector process positive regulation of production of molecular mediator of immune response regulation of production of molecular mediator of immune response positive regulation of immune effector process cytokine production involved in immune response production of molecular mediator of immune response positive regulation of cytokine production involved in immune response	membrane-bounded organelle intracellular organelle intracellular anatomical structure mitochondrion organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle cytosol membrane microdomain membrane raft membrane mitochondrial membrane organelle outer membrane membrane-bounded organelle intracellular organelle intracellular anatomical structure mitochondrion organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle cytosol membrane microdomain membrane raft membrane mitochondrial membrane organelle outer membrane organelle envelope bounding membrane of organelle mitochondrial envelope mitochondrial outer membrane outer membrane organelle membrane

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A [auth N]	IPR043129	ATPase, nucleotide binding domain	Homologous Superfamily
A [auth N]	IPR022673	Hexokinase, C-terminal	Domain
A [auth N]	IPR019807	Hexokinase, binding site	Binding Site
A [auth N]	IPR001312	Hexokinase	Family
A [auth N]	IPR022672	Hexokinase, N-terminal	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A [auth N]	Pharos: P19367	Charcot-Marie-Tooth disease Charcot-Marie-Tooth disease type 4G non-spherocytic hemolytic anemia due to hexokinase deficiency retinitis pigmentosa 79 peripheral neuropathy type 2 diabetes mellitus Diabetes Mellitus, Experimental myocardial ischemia pancreatic intraductal papillary-mucinous carcinoma congenital anemia dermatomyositis intellectual disability medulloblastoma neurodegenerative disease ovarian cancer Charcot-Marie-Tooth disease Charcot-Marie-Tooth disease type 4G non-spherocytic hemolytic anemia due to hexokinase deficiency retinitis pigmentosa 79 peripheral neuropathy type 2 diabetes mellitus Diabetes Mellitus, Experimental myocardial ischemia pancreatic intraductal papillary-mucinous carcinoma congenital anemia dermatomyositis intellectual disability medulloblastoma neurodegenerative disease ovarian cancer pancreatic intraductal papillary-mucinous neoplasm with low grade dysplasia psoriasis schizophrenia vascular disease Charcot-Marie-Tooth disease type 4 coronary artery disease esophageal adenocarcinoma lung adenocarcinoma motor peripheral neuropathy non-alcoholic fatty liver disease pancreatic ductal adenocarcinoma peripheral nervous system disease retinitis pigmentosa astrocytic tumor maturity-onset diabetes of the young

Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage

Chains	Enzyme Name	Description	Catalytic Residues
A [auth N]	hexokinase (type I) M-CSA #696	Hexokinase catalyses the first step of glycolysis, the phosphorylation of glucose by ATP to produce glucose 6-phosphate (G6P). There are four isozymes of hexokinase in vertebrates where in brain tissue and the red blood cell, type I hexokinase regulates glucose metabolism. As well as phosphorylating glucose, it's substrates also include mannose and fructose. Yeast Hexokinase is smaller than their vertebrate counterparts but also have isozymes (3). Yeast hexokinase PII has significant structural identity to the mammalian form of the enzyme and also shares the same catalytic residues, therefore thought to have a similar mechanism.	Defined by 3 residues: ARG:A-539 [auth N-539]SER:A-603 [auth N-603]ASP:A-657 [auth N-657] \| Explore in 3D: M-CSA Motif Definition Search M-CSA Motif EC: 2.7.1.1 (PDB Primary Data) Search M-CSA Motif + EC 2.7.1.1

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.