Annotations: 1DE3

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1de3a_	Alpha and beta proteins (a+b)	Microbial ribonucleases	Microbial ribonucleases	Ribotoxin	alpha-Sarcin	(Aspergillus giganteus ) [TaxId: 5060 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	Ribotoxin	8021241	4001844	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Microbial ribonucleases	8033621	3001847	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF00545	e1de3A1	A: a+b two layers	X: Microbial ribonucleases-like	H: Microbial ribonucleases (From Topology)	T: Microbial ribonucleases	F: PF00545	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.10.450.30	Alpha Beta	Roll	Nuclear Transport Factor 2	Chain: A,	CATH (4.3.0)

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	RIBONUCLEASE ALPHA-SARCIN	endonuclease activity nuclease activity hydrolase activity, acting on ester bonds catalytic activity, acting on RNA RNA endonuclease activity catalytic activity, acting on a nucleic acid RNA nuclease activity hydrolase activity catalytic activity lyase activity rRNA endonuclease activity RNA binding binding organic cyclic compound binding endonuclease activity nuclease activity hydrolase activity, acting on ester bonds catalytic activity, acting on RNA RNA endonuclease activity catalytic activity, acting on a nucleic acid RNA nuclease activity hydrolase activity catalytic activity lyase activity rRNA endonuclease activity RNA binding binding organic cyclic compound binding nucleic acid binding	regulation of cellular process cellular biosynthetic process organonitrogen compound biosynthetic process negative regulation of biological process negative regulation of cellular metabolic process regulation of primary metabolic process post-transcriptional regulation of gene expression negative regulation of translation organic substance metabolic process organic substance biosynthetic process negative regulation of cellular process protein metabolic process negative regulation of protein metabolic process negative regulation of macromolecule metabolic process regulation of cellular process cellular biosynthetic process organonitrogen compound biosynthetic process negative regulation of biological process negative regulation of cellular metabolic process regulation of primary metabolic process post-transcriptional regulation of gene expression negative regulation of translation organic substance metabolic process organic substance biosynthetic process negative regulation of cellular process protein metabolic process negative regulation of protein metabolic process negative regulation of macromolecule metabolic process regulation of biosynthetic process regulation of biological process organonitrogen compound metabolic process cellular metabolic process regulation of protein metabolic process negative regulation of metabolic process biological regulation primary metabolic process regulation of cellular metabolic process regulation of macromolecule metabolic process translation gene expression macromolecule biosynthetic process negative regulation of cellular biosynthetic process regulation of cellular biosynthetic process regulation of gene expression negative regulation of gene expression biosynthetic process negative regulation of cytoplasmic translation negative regulation of biosynthetic process regulation of translation negative regulation of macromolecule biosynthetic process cytoplasmic translation metabolic process cellular process regulation of macromolecule biosynthetic process macromolecule metabolic process regulation of cytoplasmic translation regulation of metabolic process	extracellular region cellular anatomical entity

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR016191	Ribonuclease/ribotoxin	Homologous Superfamily
A	IPR048269	Ribonuclease ribotoxin, alpha-sarcin type	Family
A	IPR004025	Fungal ribotoxin	Family

Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage

Chains	Enzyme Name	Description	Catalytic Residues
A	rRNA endonuclease M-CSA #832	Alpha-sarcin is an extracellular cytotoxic protein produced by the mold Aspergillus giganteus. It is a specific ribonuclease which inhibits protein synthesis by cleaving a specific phosphodiester bond in the highly conserved sarcin-ricin loop of the 28S rRNA. The enzyme is a member of a distinctive family of fungal ribonucleases known as ribotoxins, which are produced by different Aspergillus species. The ribotoxins show high sequence homology to each other and are also related to the RNAse T1 subfamily of microbial ribonucleases.	Defined by 3 residues: HIS:A-50GLU:A-96HIS:A-137 \| Explore in 3D: M-CSA Motif Definition Search M-CSA Motif EC: 3.1.27.10 (PDB Primary Data) Search M-CSA Motif + EC 3.1.27.10

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.