Annotations: 1C3C

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1c3ca_	All alpha proteins	L-aspartase-like	L-aspartase-like	L-aspartase/fumarase	Adenylosuccinate lyase	(Thermotoga maritima ) [TaxId: 2336 ],	SCOPe (2.08)
B	d1c3cb_	All alpha proteins	L-aspartase-like	L-aspartase-like	L-aspartase/fumarase	Adenylosuccinate lyase	(Thermotoga maritima ) [TaxId: 2336 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	L-aspartase/fumarase	8026461	4001433	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	L-aspartase-like	8038840	3001572	SCOP2 (2022-06-29)
B	SCOP2B Superfamily	L-aspartase-like	8038840	3001572	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Adenylsuccinate_lyase_1	e1c3cA3	A: alpha arrays	X: L-aspartase N-terminal domain-like (From Topology)	H: L-aspartase N-terminal domain-like (From Topology)	T: L-aspartase N-terminal domain-like	F: Adenylsuccinate_lyase_1	ECOD (1.6)
A	ADSL_C	e1c3cA2	A: alpha arrays	X: L-aspartase C-terminal domain-like (From Homology)	H: L-aspartase C-terminal domain-like	T: L-aspartase C-terminal domain-like	F: ADSL_C	ECOD (1.6)
A	Lyase_1_C	e1c3cA1	A: alpha arrays	X: L-aspartase middle domain-like	H: L-aspartase middle domain-like (From Topology)	T: L-aspartase middle domain-like	F: Lyase_1_C	ECOD (1.6)
B	Adenylsuccinate_lyase_1	e1c3cB3	A: alpha arrays	X: L-aspartase N-terminal domain-like (From Topology)	H: L-aspartase N-terminal domain-like (From Topology)	T: L-aspartase N-terminal domain-like	F: Adenylsuccinate_lyase_1	ECOD (1.6)
B	ADSL_C	e1c3cB2	A: alpha arrays	X: L-aspartase C-terminal domain-like (From Homology)	H: L-aspartase C-terminal domain-like	T: L-aspartase C-terminal domain-like	F: ADSL_C	ECOD (1.6)
B	Lyase_1_C	e1c3cB1	A: alpha arrays	X: L-aspartase middle domain-like	H: L-aspartase middle domain-like (From Topology)	T: L-aspartase middle domain-like	F: Lyase_1_C	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.10.275.10	Mainly Alpha	Orthogonal Bundle	Fumarase C	Chain B, domain 1	CATH (4.3.0)
A	1.20.200.10	Mainly Alpha	Up-down Bundle	Fumarase C	Chain A, domain 2	CATH (4.3.0)
A	1.10.40.30	Mainly Alpha	Orthogonal Bundle	Ribonucleotide Reductase Protein R1	domain 1	CATH (4.3.0)
B	1.10.275.10	Mainly Alpha	Orthogonal Bundle	Fumarase C	Chain B, domain 1	CATH (4.3.0)
B	1.20.200.10	Mainly Alpha	Up-down Bundle	Fumarase C	Chain A, domain 2	CATH (4.3.0)
B	1.10.40.30	Mainly Alpha	Orthogonal Bundle	Ribonucleotide Reductase Protein R1	domain 1	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF00206	Lyase (Lyase_1)	Lyase	-	Domain
A, B	PF10397	Adenylosuccinate lyase C-terminus (ADSL_C)	Adenylosuccinate lyase C-terminus	This is the C-terminal seven alpha helices of the structure whose full length represents the enzyme adenylosuccinate lyase. This sequence lies C-terminal to the conserved motif necessary for beta-elimination reactions [1], Adenylosuccinate lyase cata ...	This is the C-terminal seven alpha helices of the structure whose full length represents the enzyme adenylosuccinate lyase. This sequence lies C-terminal to the conserved motif necessary for beta-elimination reactions [1], Adenylosuccinate lyase catalyses two steps in the synthesis of purine nucleotides: the conversion of succinylaminoimidazole-carboxamide ribotide into aminoimidazole-carboxamide ribotide, the eighth step of the de novo pathway, and the formation of adenosine monophosphate (AMP) from adenylosuccinate, the second step in the conversion of inosine monophosphate into AMP [2].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	PROTEIN (ADENYLOSUCCINATE LYASE)	N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity amidine-lyase activity lyase activity catalytic activity carbon-nitrogen lyase activity (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity	ribose phosphate metabolic process IMP metabolic process purine ribonucleoside monophosphate biosynthetic process nucleotide biosynthetic process organonitrogen compound biosynthetic process carbohydrate derivative metabolic process purine ribonucleotide biosynthetic process nucleobase-containing compound metabolic process ribonucleotide biosynthetic process purine nucleoside monophosphate biosynthetic process purine ribonucleoside monophosphate metabolic process purine nucleotide metabolic process organic substance metabolic process ribonucleotide metabolic process ribonucleoside monophosphate metabolic process ribose phosphate metabolic process IMP metabolic process purine ribonucleoside monophosphate biosynthetic process nucleotide biosynthetic process organonitrogen compound biosynthetic process carbohydrate derivative metabolic process purine ribonucleotide biosynthetic process nucleobase-containing compound metabolic process ribonucleotide biosynthetic process purine nucleoside monophosphate biosynthetic process purine ribonucleoside monophosphate metabolic process purine nucleotide metabolic process organic substance metabolic process ribonucleotide metabolic process ribonucleoside monophosphate metabolic process nucleobase-containing small molecule metabolic process purine-containing compound biosynthetic process purine nucleoside monophosphate metabolic process phosphate-containing compound metabolic process organic substance biosynthetic process small molecule metabolic process phosphorus metabolic process ribose phosphate biosynthetic process nucleoside monophosphate metabolic process nucleoside phosphate metabolic process carbohydrate derivative biosynthetic process organonitrogen compound metabolic process cellular metabolic process primary metabolic process nucleotide metabolic process nucleobase-containing compound biosynthetic process nucleoside monophosphate biosynthetic process purine-containing compound metabolic process organic cyclic compound metabolic process IMP biosynthetic process biosynthetic process organophosphate metabolic process organophosphate biosynthetic process organic cyclic compound biosynthetic process nucleoside phosphate biosynthetic process metabolic process cellular process 'de novo' IMP biosynthetic process purine ribonucleotide metabolic process ribonucleoside monophosphate biosynthetic process purine nucleotide biosynthetic process 'de novo' AMP biosynthetic process AMP metabolic process AMP biosynthetic process	intracellular anatomical structure cytoplasm cellular anatomical entity cytosol

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR000362	Fumarate lyase family	Family
A, B	IPR022761	Fumarate lyase, N-terminal	Domain
A, B	IPR004769	Adenylosuccinate lyase	Family
A, B	IPR024083	Fumarase/histidase, N-terminal	Homologous Superfamily
A, B	IPR008948	L-Aspartase-like	Homologous Superfamily
A, B	IPR020557	Fumarate lyase, conserved site	Conserved Site
A, B	IPR019468	Adenylosuccinate lyase C-terminal	Domain

Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage

Chains	Enzyme Name	Description	Catalytic Residues
A, B	adenylosuccinate lyase M-CSA #80	Adenylosuccinate lyase catalyses two similar but separate reactions in the de novo purine synthesis pathway. In the first reaction is converts 5-aminoimidazole-(N-succinylcarboxyamide) ribotide into 5-aminoimidazole-4-carboxyamide ribotide in the ninth step of the synthesis of inosine monophosphate. In the second reaction the enzyme converts adenylosuccinate into adenosine monophospate which occurs four steps after the first reaction. Adenylosuccinate lyase helps provide the majority of purine nucleotides required for DNA replication as well as playing a role in cellular metabolism as an enzyme in the purine nucleotide cycle. The purine nucleotide cycle controls both the amounts of available citric acid intermediates and the amount of free AMP. Mutations in the enzyme leads to severe clinical consequences including mental retardation with autistic features.	Defined by 6 residues: HIS:A_2-67 [auth A_2-68]THR:A-139 [auth A-140]HIS:A-140 [auth A-141]SER:B-262 [auth B-263]LYS:B-267 [auth B-268]GLU:B-274 [auth B-275] \| Explore in 3D: M-CSA Motif Definition Search M-CSA Motif EC: 4.3.2.2 (PDB Primary Data) Search M-CSA Motif + EC 4.3.2.2

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