1B9X

STRUCTURAL ANALYSIS OF PHOSDUCIN AND ITS PHOSPHORYLATION-REGULATED INTERACTION WITH TRANSDUCIN

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Membrane Protein Annotation: OPM

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1b9xa_	All beta proteins	7-bladed beta-propeller	WD40 repeat-like	WD40-repeat	beta1-subunit of the signal-transducing G protein heterotrimer	(Bos taurus ) [TaxId: 9913 ],	SCOPe (2.08)
B	d1b9xb_	All alpha proteins	Non-globular all-alpha subunits of globular proteins	Transducin (heterotrimeric G protein), gamma chain	Transducin (heterotrimeric G protein), gamma chain	Transducin (heterotrimeric G protein), gamma chain	(Bos taurus ) [TaxId: 9913 ],	SCOPe (2.08)
C	d1b9xc_	Alpha and beta proteins (a/b)	Thioredoxin fold	Thioredoxin-like	Phosducin	Phosducin	Norway rat (Rattus norvegicus ) [TaxId: 10116 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	WD40 repeat-like	8042849	3001694	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Transducin (heterotrimeric G protein) gamma chain	8039083	3001771	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	ANAPC4_WD40_3	e1b9xA1	A: beta duplicates or obligate multimers	X: beta-propeller-like	H: beta-propeller	T: 7-bladed	F: ANAPC4_WD40_3	ECOD (1.6)
B	G-gamma	e1b9xB1	A: extended segments	X: Transducin (heterotrimeric G protein), gamma chain (From Topology)	H: Transducin (heterotrimeric G protein), gamma chain (From Topology)	T: Transducin (heterotrimeric G protein), gamma chain	F: G-gamma	ECOD (1.6)
C	Phosducin	e1b9xC1	A: a+b three layers	X: Thioredoxin-like	H: Thioredoxin-like (From Topology)	T: Thioredoxin-like	F: Phosducin	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.130.10.10	Mainly Beta	7 Propeller	Methylamine Dehydrogenase	Chain H	CATH (4.3.0)
B	4.10.260.10	Few Secondary Structures	Irregular	G Protein Gi Gamma 2	Transducin (heterotrimeric G protein), gamma chain	CATH (4.3.0)
C	3.40.30.10	Alpha Beta	3-Layer(aba) Sandwich	Glutaredoxin	Glutaredoxin	CATH (4.3.0)
C	1.10.168.10	Mainly Alpha	Orthogonal Bundle	Phosducin	domain 2	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00400	WD domain, G-beta repeat (WD40)	WD domain, G-beta repeat	-	Repeat
B	PF00631	GGL domain (G-gamma)	GGL domain	G-protein gamma like domains (GGL) are found in the gamma subunit of the heterotrimeric G protein complex and in regulators of G protein signaling (RGS) proteins [1]. It is also found fused to an inactive Galpha in the Dictyostelium protein gbqA [2] ...	G-protein gamma like domains (GGL) are found in the gamma subunit of the heterotrimeric G protein complex and in regulators of G protein signaling (RGS) proteins [1]. It is also found fused to an inactive Galpha in the Dictyostelium protein gbqA [2]. G-gamma likely shares a common origin with the helical N-terminal unit of G-beta [2]. All organisms that posses a G-beta possess a G-gamma [2].	Domain
C	PF02114	Phosducin (Phosducin)	Phosducin	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	PROTEIN (TRANSDUCIN)	protein-containing complex binding binding protein binding enzyme binding GTPase binding pyrophosphatase activity GTPase activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity signaling receptor complex adaptor activity protein-macromolecule adaptor activity protein-containing complex binding binding protein binding enzyme binding GTPase binding pyrophosphatase activity GTPase activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity signaling receptor complex adaptor activity protein-macromolecule adaptor activity molecular adaptor activity signaling adaptor activity	cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation phospholipase C-activating G protein-coupled receptor signaling pathway cell communication G protein-coupled receptor signaling pathway cellular process signal transduction multicellular organismal process system process sensory perception of chemical stimulus cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation phospholipase C-activating G protein-coupled receptor signaling pathway cell communication G protein-coupled receptor signaling pathway cellular process signal transduction multicellular organismal process system process sensory perception of chemical stimulus sensory perception nervous system process sensory perception of taste developmental process sensory organ development eye development animal organ development retina development in camera-type eye visual system development multicellular organism development camera-type eye development anatomical structure development sensory system development system development cell population proliferation cellular response to nitrogen compound response to dopamine response to organic substance dopamine receptor signaling pathway cellular response to dopamine response to monoamine cellular response to endogenous stimulus response to catecholamine adenylate cyclase-activating dopamine receptor signaling pathway cellular response to catecholamine stimulus cellular response to organic substance response to organic cyclic compound response to organonitrogen compound response to endogenous stimulus cellular response to organic cyclic compound response to oxygen-containing compound response to nitrogen compound adenylate cyclase-activating G protein-coupled receptor signaling pathway cellular response to oxygen-containing compound cellular response to chemical stimulus cellular response to organonitrogen compound cellular response to monoamine stimulus response to chemical adenylate cyclase-modulating G protein-coupled receptor signaling pathway response to ketone response to prostaglandin response to lipid response to hormone response to prostaglandin E cellular response to ketone cellular response to hormone stimulus response to alcohol cellular response to prostaglandin stimulus cellular response to prostaglandin E stimulus cellular response to alcohol cellular response to lipid	membrane cellular anatomical entity protein-containing complex extrinsic component of plasma membrane catalytic complex plasma membrane protein complex cytoplasmic side of membrane plasma membrane membrane protein complex heterotrimeric G-protein complex side of membrane cell periphery cytoplasmic side of plasma membrane GTPase complex membrane cellular anatomical entity protein-containing complex extrinsic component of plasma membrane catalytic complex plasma membrane protein complex cytoplasmic side of membrane plasma membrane membrane protein complex heterotrimeric G-protein complex side of membrane cell periphery cytoplasmic side of plasma membrane GTPase complex extrinsic component of membrane extrinsic component of cytoplasmic side of plasma membrane membrane-bounded organelle non-motile cilium 9+0 non-motile cilium cilium neuron projection photoreceptor disc membrane photoreceptor outer segment plasma membrane bounded cell projection cell projection photoreceptor cell cilium organelle organelle membrane intracellular anatomical structure cytoplasm
B	PROTEIN (TRANSDUCIN)	protein binding binding G-protein beta-subunit binding	cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation G protein-coupled receptor signaling pathway cellular process signal transduction cell communication protein localization localization cellular localization cellular macromolecule localization cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation G protein-coupled receptor signaling pathway cellular process signal transduction cell communication protein localization localization cellular localization cellular macromolecule localization macromolecule localization multicellular organismal process cellular developmental process sensory organ development photoreceptor cell differentiation visual system development cell development eye morphogenesis multicellular organism development neurogenesis neuron differentiation cell differentiation eye photoreceptor cell differentiation anatomical structure development animal organ morphogenesis sensory system development system development photoreceptor cell development developmental process eye photoreceptor cell development eye development animal organ development nervous system development generation of neurons anatomical structure morphogenesis sensory organ morphogenesis neuron development phototransduction detection of light stimulus response to light stimulus detection of external stimulus response to external stimulus detection of abiotic stimulus detection of stimulus response to abiotic stimulus response to radiation	protein-containing complex extrinsic component of plasma membrane catalytic complex plasma membrane protein complex cytoplasmic side of membrane membrane plasma membrane membrane protein complex cellular anatomical entity heterotrimeric G-protein complex side of membrane cell periphery cytoplasmic side of plasma membrane GTPase complex protein-containing complex extrinsic component of plasma membrane catalytic complex plasma membrane protein complex cytoplasmic side of membrane membrane plasma membrane membrane protein complex cellular anatomical entity heterotrimeric G-protein complex side of membrane cell periphery cytoplasmic side of plasma membrane GTPase complex extrinsic component of membrane extrinsic component of cytoplasmic side of plasma membrane membrane-bounded organelle non-motile cilium 9+0 non-motile cilium cilium neuron projection photoreceptor disc membrane photoreceptor outer segment plasma membrane bounded cell projection cell projection photoreceptor cell cilium organelle organelle membrane
C	PROTEIN (PHOSDUCIN)	-	cellular response to stimulus regulation of biological process regulation of signaling regulation of cellular process response to stimulus signaling biological regulation regulation of signal transduction cell communication regulation of response to stimulus regulation of cell communication G protein-coupled receptor signaling pathway cellular process signal transduction cellular response to stimulus regulation of biological process regulation of signaling regulation of cellular process response to stimulus signaling biological regulation regulation of signal transduction cell communication regulation of response to stimulus regulation of cell communication G protein-coupled receptor signaling pathway cellular process signal transduction regulation of G protein-coupled receptor signaling pathway multicellular organismal process sensory perception of light stimulus system process sensory perception nervous system process visual perception	intracellular anatomical structure cytoplasm cellular anatomical entity cytosol photoreceptor inner segment membrane-bounded organelle intracellular organelle organelle nucleus intracellular membrane-bounded organelle non-motile cilium 9+0 non-motile cilium cilium neuron projection intracellular anatomical structure cytoplasm cellular anatomical entity cytosol photoreceptor inner segment membrane-bounded organelle intracellular organelle organelle nucleus intracellular membrane-bounded organelle non-motile cilium 9+0 non-motile cilium cilium neuron projection photoreceptor outer segment plasma membrane bounded cell projection cell projection photoreceptor cell cilium

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR019775	WD40 repeat, conserved site	Conserved Site
A	IPR016346	Guanine nucleotide-binding protein, beta subunit	Family
A	IPR036322	WD40-repeat-containing domain superfamily	Homologous Superfamily
A	IPR001632	G-protein, beta subunit	Domain
A	IPR001680	WD40 repeat	Repeat
A	IPR020472	G-protein beta WD-40 repeat	Repeat
A	IPR015943	WD40/YVTN repeat-like-containing domain superfamily	Homologous Superfamily
B	IPR036284	G-protein gamma-like domain superfamily	Homologous Superfamily
B	IPR015898	G-protein gamma-like domain	Domain
B	IPR001770	G-protein, gamma subunit	Family
C	IPR024253	Phosducin, thioredoxin-like domain	Domain
C	IPR023196	Phosducin, N-terminal domain superfamily	Homologous Superfamily
C	IPR001200	Phosducin	Family
C	IPR036249	Thioredoxin-like superfamily	Homologous Superfamily

Membrane Protein Annotation: OPM OPM Database Homepage

Chains	External Link	Type	Class	Superfamily	Family
B	OPM	Monotopic/peripheral	Alpha/Beta monotopic/peripheral	Thioredoxin-like	Phosducin

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.