1A4L

ADA STRUCTURE COMPLEXED WITH DEOXYCOFORMYCIN AT PH 7.0

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Structure Motif: Primary M-CSA Annotation

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1a4la_	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	Metallo-dependent hydrolases	Adenosine/AMP deaminase	Adenosine deaminase (ADA)	house mouse (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
B	d1a4lb_	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	Metallo-dependent hydrolases	Adenosine/AMP deaminase	Adenosine deaminase (ADA)	house mouse (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
C	d1a4lc_	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	Metallo-dependent hydrolases	Adenosine/AMP deaminase	Adenosine deaminase (ADA)	house mouse (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
D	d1a4ld_	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	Metallo-dependent hydrolases	Adenosine/AMP deaminase	Adenosine deaminase (ADA)	house mouse (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Metallo-dependent hydrolases	8043396	3000428	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Metallo-dependent hydrolases	8043396	3000428	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Metallo-dependent hydrolases	8043396	3000428	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Metallo-dependent hydrolases	8043396	3000428	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF00962	e1a4lA1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: PF00962	ECOD (1.6)
B	PF00962	e1a4lB1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: PF00962	ECOD (1.6)
C	PF00962	e1a4lC1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: PF00962	ECOD (1.6)
D	PF00962	e1a4lD1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: PF00962	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.20.20.140	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Metal-dependent hydrolases	CATH (4.3.0)
B	3.20.20.140	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Metal-dependent hydrolases	CATH (4.3.0)
C	3.20.20.140	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Metal-dependent hydrolases	CATH (4.3.0)
D	3.20.20.140	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Metal-dependent hydrolases	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF00962	Adenosine deaminase (A_deaminase)	Adenosine deaminase	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	ADENOSINE DEAMINASE	binding organic cyclic compound binding nucleoside binding heterocyclic compound binding carbohydrate derivative binding purine nucleoside binding small molecule binding hydrolase activity deaminase activity catalytic activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds 2'-deoxyadenosine deaminase activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines adenosine deaminase activity binding organic cyclic compound binding nucleoside binding heterocyclic compound binding carbohydrate derivative binding purine nucleoside binding small molecule binding hydrolase activity deaminase activity catalytic activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds 2'-deoxyadenosine deaminase activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines adenosine deaminase activity cation binding transition metal ion binding zinc ion binding ion binding metal ion binding	heart process multicellular organismal process system process regulation of biological process regulation of heart contraction regulation of system process biological regulation positive regulation of biological process positive regulation of heart rate positive regulation of blood circulation positive regulation of heart contraction circulatory system process regulation of blood circulation positive regulation of multicellular organismal process heart process multicellular organismal process system process regulation of biological process regulation of heart contraction regulation of system process biological regulation positive regulation of biological process positive regulation of heart rate positive regulation of blood circulation positive regulation of heart contraction circulatory system process regulation of blood circulation positive regulation of multicellular organismal process regulation of multicellular organismal process regulation of biological quality heart contraction blood circulation regulation of heart rate cellular response to stimulus positive regulation of cell communication regulation of cellular process positive regulation of signal transduction response to stimulus signaling regulation of signal transduction intracellular signal transduction cell communication positive regulation of response to stimulus regulation of intracellular signal transduction intracellular signaling cassette regulation of calcium-mediated signaling positive regulation of calcium-mediated signaling signal transduction positive regulation of cellular process regulation of signaling positive regulation of intracellular signal transduction regulation of response to stimulus calcium-mediated signaling regulation of cell communication cellular process positive regulation of signaling response to chemical response to inorganic substance developmental process placenta development animal organ development anatomical structure development regulation of B cell apoptotic process homeostatic process leukocyte apoptotic process negative regulation of biological process regulation of programmed cell death lymphocyte apoptotic process negative regulation of leukocyte apoptotic process negative regulation of mature B cell apoptotic process negative regulation of cellular process regulation of immune system process regulation of leukocyte apoptotic process regulation of lymphocyte apoptotic process mature B cell apoptotic process negative regulation of programmed cell death regulation of apoptotic process B cell apoptotic process cell death negative regulation of B cell apoptotic process B cell homeostasis negative regulation of apoptotic process multicellular organismal-level homeostasis leukocyte homeostasis immune system process homeostasis of number of cells regulation of mature B cell apoptotic process lymphocyte homeostasis apoptotic process programmed cell death negative regulation of lymphocyte apoptotic process regulation of body fluid levels secretion by tissue secretion transport mucus secretion establishment of localization localization body fluid secretion regulation of penile erection regulation of reproductive process reproductive process multicellular organismal reproductive process mating behavior negative regulation of penile erection behavior negative regulation of reproductive process reproductive behavior negative regulation of multicellular organismal process penile erection copulation negative regulation of signaling negative regulation of G protein-coupled receptor signaling pathway negative regulation of cell communication regulation of G protein-coupled receptor signaling pathway purinergic nucleotide receptor signaling pathway cell surface receptor signaling pathway negative regulation of response to stimulus G protein-coupled purinergic receptor signaling pathway negative regulation of signal transduction regulation of adenosine receptor signaling pathway G protein-coupled receptor signaling pathway negative regulation of adenosine receptor signaling pathway G protein-coupled adenosine receptor signaling pathway regulation of leukocyte differentiation cellular developmental process regulation of developmental process regulation of leukocyte activation cell development regulation of cell activation mononuclear cell differentiation regulation of cell differentiation lymphocyte activation lymphocyte differentiation regulation of cell development leukocyte activation regulation of T cell differentiation multicellular organism development T cell activation cell differentiation T cell differentiation regulation of lymphocyte differentiation regulation of multicellular organismal development hemopoiesis cell activation regulation of T cell activation regulation of hemopoiesis leukocyte differentiation regulation of lymphocyte activation adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains positive regulation of germinal center formation positive regulation of adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains regulation of germinal center formation positive regulation of developmental process regulation of adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains positive regulation of immune response germinal center formation positive regulation of adaptive immune response positive regulation of immune system process regulation of anatomical structure morphogenesis immune response regulation of adaptive immune response adaptive immune response anatomical structure morphogenesis anatomical structure formation involved in morphogenesis regulation of immune response purine deoxyribonucleoside monophosphate catabolic process deoxyribonucleoside monophosphate catabolic process catabolic process deoxyribonucleotide catabolic process dAMP catabolic process carbohydrate derivative metabolic process organophosphate catabolic process dAMP metabolic process nucleoside phosphate catabolic process deoxyribose phosphate catabolic process purine nucleoside monophosphate catabolic process nucleobase-containing compound metabolic process organic substance metabolic process purine nucleotide metabolic process deoxyribonucleotide metabolic process nucleobase-containing small molecule metabolic process purine nucleoside monophosphate metabolic process phosphate-containing compound metabolic process small molecule metabolic process phosphorus metabolic process nucleoside monophosphate metabolic process nucleotide catabolic process nucleoside phosphate metabolic process organonitrogen compound catabolic process purine nucleotide catabolic process purine deoxyribonucleotide catabolic process carbohydrate derivative catabolic process organonitrogen compound metabolic process nucleoside monophosphate catabolic process cellular metabolic process 2'-deoxyribonucleotide metabolic process purine deoxyribonucleoside monophosphate metabolic process primary metabolic process purine-containing compound catabolic process nucleotide metabolic process deoxyribose phosphate metabolic process deoxyribonucleoside monophosphate metabolic process organic cyclic compound catabolic process purine-containing compound metabolic process nucleobase-containing compound catabolic process organic cyclic compound metabolic process organophosphate metabolic process purine deoxyribonucleotide metabolic process metabolic process organic substance catabolic process glycosyl compound metabolic process organonitrogen compound biosynthetic process nucleoside biosynthetic process purine-containing compound biosynthetic process organic substance biosynthetic process purine ribonucleoside metabolic process carbohydrate derivative biosynthetic process glycosyl compound biosynthetic process inosine metabolic process small molecule biosynthetic process purine ribonucleoside biosynthetic process ribonucleoside biosynthetic process nucleobase-containing compound biosynthetic process nucleoside metabolic process nucleobase-containing small molecule biosynthetic process inosine biosynthetic process biosynthetic process purine nucleoside biosynthetic process organic cyclic compound biosynthetic process purine nucleoside metabolic process ribonucleoside metabolic process embryo development ending in birth or egg hatching chordate embryonic development embryo development in utero embryonic development T cell differentiation in thymus immune response-regulating cell surface receptor signaling pathway positive regulation of T cell receptor signaling pathway regulation of antigen receptor-mediated signaling pathway immune response-activating signaling pathway immune response-regulating signaling pathway immune response-activating cell surface receptor signaling pathway regulation of T cell receptor signaling pathway T cell receptor signaling pathway antigen receptor-mediated signaling pathway activation of immune response positive regulation of antigen receptor-mediated signaling pathway alpha-beta T cell differentiation alpha-beta T cell activation cell adhesion response to decreased oxygen levels response to hypoxia response to stress response to oxygen levels response to abiotic stimulus nucleoside triphosphate catabolic process purine nucleoside triphosphate catabolic process purine deoxyribonucleoside triphosphate metabolic process dATP metabolic process purine deoxyribonucleoside triphosphate catabolic process deoxyribonucleoside triphosphate metabolic process purine nucleoside triphosphate metabolic process deoxyribonucleoside triphosphate catabolic process dATP catabolic process nucleoside triphosphate metabolic process regulation of circadian sleep/wake cycle regulation of circadian sleep/wake cycle, non-REM sleep regulation of circadian rhythm circadian sleep/wake cycle, non-REM sleep regulation of behavior sleep circadian sleep/wake cycle, sleep rhythmic process negative regulation of behavior circadian sleep/wake cycle rhythmic behavior circadian rhythm negative regulation of circadian sleep/wake cycle, sleep negative regulation of circadian rhythm negative regulation of circadian sleep/wake cycle, non-REM sleep circadian behavior regulation of circadian sleep/wake cycle, sleep circadian sleep/wake cycle process cell-cell adhesion mediated by integrin regulation of cell adhesion mediated by integrin regulation of cell adhesion regulation of cell-cell adhesion mediated by integrin cell-cell adhesion cell adhesion mediated by integrin regulation of cell-cell adhesion negative regulation of locomotion regulation of cell motility regulation of leukocyte migration leukocyte migration negative regulation of leukocyte migration negative regulation of immune system process regulation of locomotion regulation of cell migration negative regulation of cell motility cell migration cell motility negative regulation of cell migration locomotion positive regulation of cell population proliferation positive regulation of B cell proliferation leukocyte proliferation positive regulation of lymphocyte activation positive regulation of cell activation regulation of B cell proliferation lymphocyte proliferation regulation of B cell activation positive regulation of leukocyte activation positive regulation of mononuclear cell proliferation regulation of lymphocyte proliferation regulation of leukocyte proliferation cell population proliferation B cell activation positive regulation of lymphocyte proliferation B cell proliferation positive regulation of B cell activation regulation of cell population proliferation mononuclear cell proliferation positive regulation of leukocyte proliferation regulation of mononuclear cell proliferation nucleotide biosynthetic process nucleotide salvage purine nucleotide salvage cellular metabolic compound salvage purine-containing compound salvage organophosphate biosynthetic process nucleoside phosphate biosynthetic process purine nucleotide biosynthetic process response to nutrient levels response to organic substance response to nutrient response to oxygen-containing compound response to vitamin E response to vitamin response to organic cyclic compound ribose phosphate metabolic process ribonucleotide catabolic process AMP metabolic process purine ribonucleoside monophosphate catabolic process purine ribonucleoside monophosphate metabolic process ribonucleotide metabolic process ribonucleoside monophosphate metabolic process ribonucleoside monophosphate catabolic process purine ribonucleotide catabolic process AMP catabolic process purine ribonucleotide metabolic process thymocyte apoptotic process T cell apoptotic process regulation of alpha-beta T cell activation positive regulation of T cell differentiation positive regulation of T cell activation positive regulation of cell development positive regulation of alpha-beta T cell activation regulation of leukocyte cell-cell adhesion positive regulation of alpha-beta T cell differentiation positive regulation of leukocyte cell-cell adhesion regulation of alpha-beta T cell differentiation leukocyte cell-cell adhesion positive regulation of cell-cell adhesion positive regulation of hemopoiesis positive regulation of cell differentiation positive regulation of cell adhesion positive regulation of leukocyte differentiation positive regulation of lymphocyte differentiation hepaticobiliary system development gland development liver development system development deoxyadenosine catabolic process deoxyribonucleoside metabolic process purine nucleoside catabolic process deoxyadenosine metabolic process purine deoxyribonucleoside catabolic process purine deoxyribonucleoside metabolic process glycosyl compound catabolic process nucleobase-containing small molecule catabolic process nucleoside catabolic process deoxyribonucleoside catabolic process small molecule catabolic process purine ribonucleoside monophosphate biosynthetic process purine ribonucleotide biosynthetic process ribonucleotide biosynthetic process purine nucleoside monophosphate biosynthetic process GMP metabolic process ribose phosphate biosynthetic process GMP salvage GMP biosynthetic process purine ribonucleotide salvage nucleoside monophosphate biosynthetic process ribonucleoside monophosphate biosynthetic process purine nucleobase biosynthetic process hypoxanthine biosynthetic process nucleobase metabolic process hypoxanthine salvage purine nucleobase salvage purine nucleobase metabolic process nucleobase biosynthetic process hypoxanthine metabolic process IMP salvage IMP metabolic process IMP biosynthetic process digestive system development tube development digestive tract development embryonic organ development embryonic digestive tract development smooth muscle contraction muscle contraction muscle system process respiratory tube development lung development respiratory system development regulation of defense response regulation of response to external stimulus negative regulation of inflammatory response defense response response to external stimulus regulation of inflammatory response negative regulation of response to external stimulus regulation of response to stress negative regulation of defense response inflammatory response xanthine metabolic process xanthine biosynthetic process adenosine metabolic process inhibition of non-skeletal tissue mineralization anatomical structure homeostasis tissue homeostasis positive regulation of T cell differentiation in thymus regulation of T cell differentiation in thymus trophectodermal cell differentiation blastocyst formation blastocyst development nitrogen compound transport histamine transport organic substance transport histamine secretion amide catabolic process amide metabolic process regulation of localization regulation of transport regulation of mucus secretion negative regulation of transport negative regulation of mucus secretion regulation of secretion negative regulation of secretion AMP biosynthetic process AMP salvage germinal center B cell differentiation mature B cell differentiation B cell activation involved in immune response cell activation involved in immune response B cell differentiation leukocyte activation involved in immune response lymphocyte activation involved in immune response mature B cell differentiation involved in immune response immune effector process positive regulation of smooth muscle contraction regulation of muscle system process regulation of muscle contraction regulation of smooth muscle contraction positive regulation of muscle contraction adenosine catabolic process ribonucleoside catabolic process purine ribonucleoside catabolic process allantoin metabolic process negative regulation of thymocyte apoptotic process regulation of T cell apoptotic process negative regulation of T cell apoptotic process regulation of thymocyte apoptotic process response to organonitrogen compound response to nitrogen compound response to purine-containing compound immune system development hematopoietic or lymphoid organ development tissue development Peyer's patch development mucosa-associated lymphoid tissue development lung alveolus development	cell periphery cell surface membrane plasma membrane cellular anatomical entity external side of plasma membrane side of membrane intracellular anatomical structure cytoplasm cytosol somatodendritic compartment cell body neuronal cell body extracellular region cell periphery cell surface membrane plasma membrane cellular anatomical entity external side of plasma membrane side of membrane intracellular anatomical structure cytoplasm cytosol somatodendritic compartment cell body neuronal cell body extracellular region extracellular space anchoring junction cell junction membrane-bounded organelle intracellular organelle organelle lytic vacuole vacuole intracellular membrane-bounded organelle lysosome neuron projection cytoplasm neuron projection cytoplasmic region plasma membrane bounded cell projection cytoplasm plasma membrane bounded cell projection dendrite cytoplasm cell projection dendrite dendritic tree vesicle lumen membrane-enclosed lumen intracellular vesicle cytoplasmic vesicle lumen intracellular organelle lumen organelle lumen cytoplasmic vesicle vesicle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR006330	Adenosine/adenine deaminase	Family
A, B, C, D	IPR006650	Adenosine/AMP deaminase active site	Active Site
A, B, C, D	IPR001365	Adenosine deaminase domain	Domain
A, B, C, D	IPR032466	Metal-dependent hydrolase	Homologous Superfamily
A, B, C, D	IPR028893	Adenosine deaminase	Family

Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage

Chains	Enzyme Name	Description	Catalytic Residues
A	adenosine deaminase M-CSA #376	Adenosine deaminase (ADA) is the critical enzyme in purine metabolism, which catalyses the irreversible deamination of adenosine and deoxyadenosine to their respective inosine product. It is present in virtually all mammalian cells and has a central role in maintaining immune competence. Genetic deficiency of ADA in humans is associated with severe combined immunodeficiency disease, whereas abnormally high level of ADA is involved in a variety of other diseases including acquired immunodeficiency syndrome (AIDS), tuberculosis, Parkinson's disease, anemia, various lymphomas and leukemias. ADA is therefore regarded as an important therapeutic target and the detailed knowledge of its catalytic mechanism is of high significance in drug design.	Defined by 6 residues: HIS:A-12 [auth A-15]HIS:A-14 [auth A-17]HIS:A-211 [auth A-214]GLU:A-214 [auth A-217]HIS:A-235 [auth A-238]ASP:A-292 [auth A-295] \| Explore in 3D: M-CSA Motif Definition Search M-CSA Motif EC: 3.5.4.4 (PDB Primary Data) Search M-CSA Motif + EC 3.5.4.4

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.