1A2O

STRUCTURAL BASIS FOR METHYLESTERASE CHEB REGULATION BY A PHOSPHORYLATION-ACTIVATED DOMAIN

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1a2oa1	Alpha and beta proteins (a/b)	Flavodoxin-like	CheY-like	CheY-related	Methylesterase CheB, N-terminal domain	(Salmonella enterica subsp. enterica serovar Typhimurium ) [TaxId: 90371 ],	SCOPe (2.08)
A	d1a2oa2	Alpha and beta proteins (a/b)	Methylesterase CheB, C-terminal domain	Methylesterase CheB, C-terminal domain	Methylesterase CheB, C-terminal domain	Methylesterase CheB, C-terminal domain	(Salmonella enterica subsp. enterica serovar Typhimurium ) [TaxId: 90371 ],	SCOPe (2.08)
B	d1a2ob1	Alpha and beta proteins (a/b)	Flavodoxin-like	CheY-like	CheY-related	Methylesterase CheB, N-terminal domain	(Salmonella enterica subsp. enterica serovar Typhimurium ) [TaxId: 90371 ],	SCOPe (2.08)
B	d1a2ob2	Alpha and beta proteins (a/b)	Methylesterase CheB, C-terminal domain	Methylesterase CheB, C-terminal domain	Methylesterase CheB, C-terminal domain	Methylesterase CheB, C-terminal domain	(Salmonella enterica subsp. enterica serovar Typhimurium ) [TaxId: 90371 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	CheY-related	8031670	4003632	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	CheY-like	8044048	3001156	SCOP2 (2022-06-29)
A	SCOP2B Superfamily	Methylesterase CheB C-terminal domain	8044324	3000222	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Methylesterase CheB C-terminal domain	8044324	3000222	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	CheY-like	8044048	3001156	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Response_reg	e1a2oA1	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: CheY-like	F: Response_reg	ECOD (1.6)
A	CheB_methylest	e1a2oA2	A: a/b three-layered sandwiches	X: Methylesterase CheB, C-terminal domain (From Topology)	H: Methylesterase CheB, C-terminal domain (From Topology)	T: Methylesterase CheB, C-terminal domain	F: CheB_methylest	ECOD (1.6)
B	Response_reg	e1a2oB1	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: CheY-like	F: Response_reg	ECOD (1.6)
B	CheB_methylest	e1a2oB2	A: a/b three-layered sandwiches	X: Methylesterase CheB, C-terminal domain (From Topology)	H: Methylesterase CheB, C-terminal domain (From Topology)	T: Methylesterase CheB, C-terminal domain	F: CheB_methylest	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.2300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Response regulator	CATH (4.3.0)
A	3.40.50.180	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Methylesterase CheB, C-terminal domain	CATH (4.3.0)
B	3.40.50.2300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Response regulator	CATH (4.3.0)
B	3.40.50.180	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Methylesterase CheB, C-terminal domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF01339	CheB methylesterase (CheB_methylest)	CheB methylesterase	-	Domain
A, B	PF00072	Response regulator receiver domain (Response_reg)	Response regulator receiver domain	This domain receives the signal from the sensor partner in bacterial two-component systems. It is usually found N-terminal to a DNA binding effector domain.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	CHEB METHYLESTERASE	catalytic activity, acting on a protein hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides hydrolase activity protein-glutamine glutaminase activity catalytic activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds molecular transducer activity phosphorelay response regulator activity protein-glutamate methylesterase activity hydrolase activity, acting on ester bonds carboxylic ester hydrolase activity protein methylesterase activity	chemotaxis response to stimulus taxis response to chemical response to external stimulus locomotion	intracellular anatomical structure cytoplasm cellular anatomical entity

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR008248	Protein-glutamate methylesterase/protein-glutamine glutaminase, CheB type	Family
A, B	IPR035909	Methylesterase CheB, C-terminal	Homologous Superfamily
A, B	IPR000673	Signal transduction response regulator, chemotaxis, protein-glutamate methylesterase	Domain
A, B	IPR011006	CheY-like superfamily	Homologous Superfamily
A, B	IPR001789	Signal transduction response regulator, receiver domain	Domain

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.