6HZG
BP0997, GH138 enzyme targeting pectin rhamnogalacturonan II
- PDB DOI: https://doi.org/10.2210/pdb6HZG/pdb
- Classification: HYDROLASE
- Organism(s): Phocaeicola paurosaccharolyticus
- Expression System: Escherichia coli
- Mutation(s): No 
- Deposited: 2018-10-23 Released: 2019-03-20 
- Funding Organization(s): European Research Council
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.60 Å
- R-Value Free: 0.205 
- R-Value Work: 0.154 
- R-Value Observed: 0.156 
This is version 2.0 of the entry. See complete history. 
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
---|---|---|---|---|---|
Molecule | Chains | Sequence Length | Organism | Details | Image |
BPa0997 N-ter E361S | 893 | Phocaeicola paurosaccharolyticus | Mutation(s): 0  | ||
UniProt | |||||
Find proteins for A0A4V8GZY5 (Phocaeicola paurosaccharolyticus) Explore A0A4V8GZY5  Go to UniProtKB:  A0A4V8GZY5 | |||||
Entity Groups   | |||||
Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
UniProt Group | A0A4V8GZY5 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
Ligands 4 Unique | |||||
---|---|---|---|---|---|
ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
GTR Query on GTR | C [auth A], D [auth A] | beta-D-galactopyranuronic acid C6 H10 O7 AEMOLEFTQBMNLQ-DTEWXJGMSA-N | |||
ADA Query on ADA | B [auth A] | alpha-D-galactopyranuronic acid C6 H10 O7 AEMOLEFTQBMNLQ-BKBMJHBISA-N | |||
CL Query on CL | E [auth A] | CHLORIDE ION Cl VEXZGXHMUGYJMC-UHFFFAOYSA-M | |||
NA Query on NA | F [auth A] | SODIUM ION Na FKNQFGJONOIPTF-UHFFFAOYSA-N |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.60 Å
- R-Value Free: 0.205 
- R-Value Work: 0.154 
- R-Value Observed: 0.156 
- Space Group: P 2 21 21
Unit Cell:
Length ( Å ) | Angle ( ˚ ) |
---|---|
a = 55.298 | α = 90 |
b = 68.379 | β = 90 |
c = 220.158 | γ = 90 |
Software Name | Purpose |
---|---|
REFMAC | refinement |
xia2 | data reduction |
Aimless | data scaling |
pointless | data scaling |
PHASER | phasing |
Coot | model building |
MolProbity | model building |
Entry History & Funding Information
Deposition Data
- Released Date: 2019-03-20  Deposition Author(s): Basle, A., Cartmell, A., Labourel, A., Gilbert, H.
Funding Organization | Location | Grant Number |
---|---|---|
European Research Council | United Kingdom | 322820 |
Revision History (Full details and data files)
- Version 1.0: 2019-03-20
Type: Initial release - Version 1.1: 2019-03-27
Changes: Data collection, Database references - Version 1.2: 2019-05-22
Changes: Data collection, Database references - Version 2.0: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Atomic model, Data collection, Derived calculations, Structure summary