3LFI

Crystal structure of fructosyltransferase (wild-type) from A. japonicus in complex with glucose

PDB DOI: https://doi.org/10.2210/pdb3LFI/pdb

Classification: HYDROLASE
Organism(s): Aspergillus japonicus
Expression System: Escherichia coli
Mutation(s): No

Deposited: 2010-01-17 Released: 2010-05-12
Deposition Author(s): Chuankhayan, P., Chen, C.J., Chiang, C.M.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.00 Å
R-Value Free: 0.255
R-Value Work: 0.226
R-Value Observed: 0.231

wwPDB Validation 3D Report Full Report

Ligand Structure Quality Assessment

This is version 1.3 of the entry. See complete history.

Literature

Crystal structure of Aspergillus japonicus fructosyltransferase complex with donor/acceptor substrates reveal complete sbusites in the active site for catalysis

Chuankhayan, P., Hsieh, C.Y., Huang, Y.C., Hsieh, Y.Y., Guan, H.H., Hsieh, Y.C., Tien, Y.C., Chen, C.D., Chaing, C.M., Chen, C.J.

To be published.

Macromolecule Content

Total Structure Weight: 138.33 kDa
Atom Count: 10,139
Modelled Residue Count: 1,268
Deposited Residue Count: 1,268
Unique protein chains: 1

Macromolecules

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(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Fructosyltransferase	A, B	634	Aspergillus japonicus	Mutation(s): 0
UniProt
Find proteins for Q1W3Z7 (Aspergillus japonicus) Explore Q1W3Z7 Go to UniProtKB: Q1W3Z7
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q1W3Z7
Sequence Annotations Expand
Reference Sequence

Small Molecules

Ligands 1 Unique
ID	Chains	Name / Formula / InChI Key	2D Diagram	3D Interactions
BGC Query on BGC Download Ideal Coordinates CCD File SDF format, chain C [auth A] SDF format, chain D [auth B] MOL2 format, chain C [auth A] MOL2 format, chain D [auth B]	C [auth A], D [auth B]	beta-D-glucopyranose C₆ H₁₂ O₆ WQZGKKKJIJFFOK-VFUOTHLCSA-N		Interactions Focus chain C [auth A] Focus chain D [auth B] Interactions & Density Focus chain C [auth A] Focus chain D [auth B]

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.00 Å
R-Value Free: 0.255
R-Value Work: 0.226
R-Value Observed: 0.231
Space Group: P 2₁ 2₁ 2₁

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 97.949	α = 90
b = 110.254	β = 90
c = 129.893	γ = 90

Software Package:

Software Name	Purpose
ADSC	data collection
CNS	refinement
HKL-2000	data reduction
HKL-2000	data scaling
CNS	phasing

Structure Validation

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Ligand Structure Quality Assessment

Entry History

Deposition Data

Released Date: 2010-05-12

Deposition Author(s):

Chuankhayan, P.

Chen, C.J.

Chiang, C.M.

Revision History (Full details and data files)

Version 1.0: 2010-05-12
Type: Initial release
Version 1.1: 2011-07-13
Changes: Version format compliance
Version 1.2: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Data collection, Derived calculations, Structure summary
Version 1.3: 2024-03-20
Changes: Data collection, Database references, Structure summary

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Help and Resources

3LFI

Crystal structure of fructosyltransferase (wild-type) from A. japonicus in complex with glucose

Crystal structure of Aspergillus japonicus fructosyltransferase complex with donor/acceptor substrates reveal complete sbusites in the active site for catalysis

Entity ID: 1

UniProt

Entity Groups

Sequence Annotations

Expand

Experimental Data

Structure Validation

Ligand Structure Quality Assessment

Deposition Data

Revision History (Full details and data files)