Download MendeleyThe Structure of Guanylate Kinase from Escherichia coli at 2.0 A resolution
Kreusch, A., Spraggon, G., Klock, H.E., McMullan, D., Vincent, J., Rodrigues, K., Lesley, S.A.To be published.
1S96
The 2.0 A X-ray structure of Guanylate Kinase from E.coli
- PDB DOI: https://doi.org/10.2210/pdb1S96/pdb
- Classification: TRANSFERASE
- Organism(s): Escherichia coli
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2004-02-03 Released: 2004-02-10
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.00 Å
- R-Value Free: 0.247
- R-Value Work: 0.195
wwPDB Validation 3D Report Full Report
This is version 1.2 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Guanylate kinase | 219 | Escherichia coli | Mutation(s): 6 Gene Names: GMK, SPOR, B3648, C4473, SF3688, S4081 EC: 2.7.4.8 |  | |
UniProt | |||||
Find proteins for P60546 (Escherichia coli (strain K12)) Explore P60546 Go to UniProtKB: P60546 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P60546 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| PO4 Query on PO4 | C [auth A], E [auth B] | PHOSPHATE ION O4 P NBIIXXVUZAFLBC-UHFFFAOYSA-K |  | ||
| UNX Query on UNX | D [auth A] | UNKNOWN ATOM OR ION X |  | ||
| Modified Residues 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| MSE Query on MSE | A, B | L-PEPTIDE LINKING | C5 H11 N O2 Se |  | MET |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.00 Å
- R-Value Free: 0.247
- R-Value Work: 0.195
- Space Group: P 21 3
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 124.552 | α = 90 |
| b = 124.552 | β = 90 |
| c = 124.552 | γ = 90 |
| Software Name | Purpose |
|---|---|
| DENZO | data reduction |
| SCALEPACK | data scaling |
| SOLVE | phasing |
| CNS | refinement |
Entry History
Deposition Data
- Released Date: 2004-02-10 Deposition Author(s): Kreusch, A., Spraggon, G., Klock, H.E., McMullan, D., Vincent, J., Rodrigues, K., Lesley, S.A.
Revision History (Full details and data files)
- Version 1.0: 2004-02-10
Type: Initial release - Version 1.1: 2008-04-29
Changes: Version format compliance - Version 1.2: 2011-07-13
Changes: Version format compliance
