Download MendeleyThe structural basis for 2'-5'/3'-5'-cGAMP synthesis by cGAS
Wu, S., Gabelli, S.B., Sohn, J.(2024) Nat Commun 15: 4012
7V0W
Structure of Ternary Complex of cGAS with dsDNA and Bound 5 -pppG(2,5 )pA
- PDB DOI: https://doi.org/10.2210/pdb7V0W/pdb
- NAKB: 7V0W
- Classification: Transferase/DNA
- Organism(s): Mus musculus, synthetic construct
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): Yes
- Deposited: 2022-05-11 Released: 2023-05-17
- Funding Organization(s): National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.66 Å
- R-Value Free: 0.238
- R-Value Work: 0.199
- R-Value Observed: 0.201
This is version 2.0 of the entry. See complete history.
Literature
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Cyclic GMP-AMP synthase | A, B [auth C] | 364 | Mus musculus | Mutation(s): 2 Gene Names: Cgas, Mb21d1 EC: 2.7.7.86 |  |
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for Q8C6L5 (Mus musculus) Explore Q8C6L5 Go to UniProtKB: Q8C6L5 | |||||
IMPC: MGI:2442261 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q8C6L5 | ||||
Sequence AnnotationsExpand | |||||
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Find similar nucleic acids by: Sequence | 3D Structure
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Length | Organism | Image | |
| Palindromic DNA18 | C [auth E], D [auth F], E [auth I], F [auth J] | 18 | synthetic construct |  | |
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 4 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| GTP (Subject of Investigation/LOI) Query on GTP | J [auth A], N [auth C] | GUANOSINE-5'-TRIPHOSPHATE C10 H16 N5 O14 P3 XKMLYUALXHKNFT-UUOKFMHZSA-N |  | ||
| AMP (Subject of Investigation/LOI) Query on AMP | I [auth A], M [auth C] | ADENOSINE MONOPHOSPHATE C10 H14 N5 O7 P UDMBCSSLTHHNCD-KQYNXXCUSA-N |  | ||
| ZN Query on ZN | G [auth A], K [auth C] | ZINC ION Zn PTFCDOFLOPIGGS-UHFFFAOYSA-N |  | ||
| MN (Subject of Investigation/LOI) Query on MN | H [auth A], L [auth C] | MANGANESE (II) ION Mn WAEMQWOKJMHJLA-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.66 Å
- R-Value Free: 0.238
- R-Value Work: 0.199
- R-Value Observed: 0.201
- Space Group: P 21 21 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 78.475 | α = 90 |
| b = 99.474 | β = 90 |
| c = 142.088 | γ = 90 |
| Software Name | Purpose |
|---|---|
| XDS | data reduction |
| Aimless | data scaling |
| MOLREP | phasing |
| REFMAC | refinement |
| PDB-REDO | refinement |
| PDB_EXTRACT | data extraction |
Entry History & Funding Information
Deposition Data
- Released Date: 2023-05-17 Deposition Author(s): Wu, S., Gabelli, S.B., Sohn, J.
| Funding Organization | Location | Grant Number |
|---|---|---|
| National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) | United States | -- |
Revision History (Full details and data files)
- Version 1.0: 2023-05-17
Type: Initial release - Version 1.1: 2023-10-25
Changes: Data collection, Refinement description - Version 1.2: 2024-05-22
Changes: Database references - Version 2.0: 2024-05-29
Changes: Atomic model, Author supporting evidence, Data collection, Derived calculations, Non-polymer description, Structure summary
