6YBT

Structure of a human 48S translational initiation complex - eIF3bgi


Experimental Data Snapshot

  • Method: ELECTRON MICROSCOPY
  • Resolution: 6.00 Å
  • Aggregation State: PARTICLE 
  • Reconstruction Method: SINGLE PARTICLE 

wwPDB Validation   3D Report Full Report


This is version 1.1 of the entry. See complete history


Literature

Structure of a human 48Stranslational initiation complex.

Brito Querido, J.Sokabe, M.Kraatz, S.Gordiyenko, Y.Skehel, J.M.Fraser, C.S.Ramakrishnan, V.

(2020) Science 369: 1220-1227

  • DOI: https://doi.org/10.1126/science.aba4904
  • Primary Citation of Related Structures:  
    6YBD, 6YBS, 6YBT, 6YBV, 6YBW, 6ZMW

  • PubMed Abstract: 

    A key step in translational initiation is the recruitment of the 43 S preinitiation complex by the cap-binding complex [eukaryotic initiation factor 4F (eIF4F)] at the 5' end of messenger RNA (mRNA) to form the 48 S initiation complex (i.e., the 48 S ). The 48 S then scans along the mRNA to locate a start codon. To understand the mechanisms involved, we used cryo-electron microscopy to determine the structure of a reconstituted human 48 S The structure reveals insights into early events of translation initiation complex assembly, as well as how eIF4F interacts with subunits of eIF3 near the mRNA exit channel in the 43 S The location of eIF4F is consistent with a slotting model of mRNA recruitment and suggests that downstream mRNA is unwound at least in part by being "pulled" through the 40 S subunit during scanning.


  • Organizational Affiliation

    MRC Laboratory of Molecular Biology, Cambridge, UK.


Macromolecules
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Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Eukaryotic translation initiation factor 3 subunit AA [auth u]1,350Homo sapiensMutation(s): 0 
UniProt & NIH Common Fund Data Resources
Find proteins for Q14152 (Homo sapiens)
Explore Q14152 
Go to UniProtKB:  Q14152
PHAROS:  Q14152
GTEx:  ENSG00000107581 
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupQ14152
Sequence Annotations
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  • Reference Sequence
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Entity ID: 2
MoleculeChains Sequence LengthOrganismDetailsImage
Eukaryotic translation initiation factor 3 subunit IB [auth 2]325Homo sapiensMutation(s): 0 
UniProt & NIH Common Fund Data Resources
Find proteins for Q13347 (Homo sapiens)
Explore Q13347 
Go to UniProtKB:  Q13347
PHAROS:  Q13347
GTEx:  ENSG00000084623 
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupQ13347
Sequence Annotations
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  • Reference Sequence
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Entity ID: 3
MoleculeChains Sequence LengthOrganismDetailsImage
Eukaryotic translation initiation factor 3 subunit BC [auth 1]814Homo sapiensMutation(s): 0 
UniProt & NIH Common Fund Data Resources
Find proteins for P55884 (Homo sapiens)
Explore P55884 
Go to UniProtKB:  P55884
PHAROS:  P55884
GTEx:  ENSG00000106263 
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupP55884
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: ELECTRON MICROSCOPY
  • Resolution: 6.00 Å
  • Aggregation State: PARTICLE 
  • Reconstruction Method: SINGLE PARTICLE 

Structure Validation

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Entry History & Funding Information

Deposition Data


Funding OrganizationLocationGrant Number
Wellcome TrustUnited KingdomWTY096570
Medical Research Council (MRC, United Kingdom)United KingdomMC_U105184332
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)United StatesR01GM092927

Revision History  (Full details and data files)

  • Version 1.0: 2020-09-16
    Type: Initial release
  • Version 1.1: 2024-05-22
    Changes: Data collection, Database references