6G04

NMR Solution Structure of Yeast TSR2(1-152) in Complex with S26A(100-119)


Experimental Data Snapshot

  • Method: SOLUTION NMR
  • Conformers Calculated: 400 
  • Conformers Submitted: 20 
  • Selection Criteria: structures with the lowest energy 

wwPDB Validation   3D Report Full Report


This is version 1.2 of the entry. See complete history


Literature

Molecular basis for disassembly of an importin:ribosomal protein complex by the escortin Tsr2.

Schutz, S.Michel, E.Damberger, F.F.Oplova, M.Pena, C.Leitner, A.Aebersold, R.Allain, F.H.Panse, V.G.

(2018) Nat Commun 9: 3669-3669

  • DOI: https://doi.org/10.1038/s41467-018-06160-x
  • Primary Citation of Related Structures:  
    6G03, 6G04

  • PubMed Abstract: 

    Disordered extensions at the termini and short internal insertions distinguish eukaryotic ribosomal proteins (r-proteins) from their anucleated archaeal counterparts. Here, we report an NMR structure of such a eukaryotic-specific segment (ESS) in the r-protein eS26 in complex with the escortin Tsr2. The structure reveals how ESS attracts Tsr2 specifically to importin:eS26 complexes entering the nucleus in order to trigger non-canonical RanGTP-independent disassembly. Tsr2 then sequesters the released eS26 and prevents rebinding to the importin, providing an alternative allosteric mechanism to terminate the process of nuclear import. Notably, a Diamond-Blackfan anemia-associated Tsr2 mutant protein is impaired in binding to ESS, unveiling a critical role for this interaction in human hematopoiesis. We propose that eS26-ESS and Tsr2 are components of a nuclear sorting system that co-evolved with the emergence of the nucleocytoplasmic barrier and transport carriers.


  • Organizational Affiliation

    Institute of Medical Microbiology, University of Zurich, 8006, Zurich, Switzerland.


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Pre-rRNA-processing protein TSR2156Saccharomyces cerevisiae S288CMutation(s): 0 
Gene Names: TSR2YLR435W
UniProt
Find proteins for Q06672 (Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Explore Q06672 
Go to UniProtKB:  Q06672
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupQ06672
Sequence Annotations
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  • Reference Sequence
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 2
MoleculeChains Sequence LengthOrganismDetailsImage
40S ribosomal protein S26-A26Saccharomyces cerevisiae S288CMutation(s): 0 
Gene Names: RPS26ARPS26YGL189CG1355
UniProt
Find proteins for P39938 (Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Explore P39938 
Go to UniProtKB:  P39938
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupP39938
Sequence Annotations
Expand
  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: SOLUTION NMR
  • Conformers Calculated: 400 
  • Conformers Submitted: 20 
  • Selection Criteria: structures with the lowest energy 

Structure Validation

View Full Validation Report



Entry History & Funding Information

Deposition Data


Funding OrganizationLocationGrant Number
European Research CouncilEURIBIO260676

Revision History  (Full details and data files)

  • Version 1.0: 2018-09-19
    Type: Initial release
  • Version 1.1: 2019-05-08
    Changes: Data collection
  • Version 1.2: 2024-05-15
    Changes: Data collection, Database references