5ZAZ

Solution structure of integrin b2 monomer tranmembrane domain in bicelle

  • Classification: CELL ADHESION
  • Organism(s): Homo sapiens
  • Expression System: Escherichia coli BL21(DE3)
  • Mutation(s): Yes 
  • Membrane Protein: Yes  OPMPDBTMMemProtMD

  • Deposited: 2018-02-09 Released: 2018-10-17 
  • Deposition Author(s): Li, H., Guo, J., Xu, C.
  • Funding Organization(s): NSFC, MOST, CAS Key Research Program of Frontier Sciences, CAS Facility-based Open Research Program, NSFC Innovative Research Group, NSFC Key Program, Ten Thousand Talent Program National Program for Support of Top-notch Young Professionals of China, STSMC Key Program, NSFC National Science Fund for Distinguished Young Scholars, STSMC Outstanding Academic Leader Program, CAS Strategic Priority Research Program

Experimental Data Snapshot

  • Method: SOLUTION NMR
  • Conformers Calculated: 100 
  • Conformers Submitted: 20 
  • Selection Criteria: target function 

wwPDB Validation   3D Report Full Report


This is version 1.3 of the entry. See complete history


Literature

Intramembrane ionic protein-lipid interaction regulates integrin structure and function.

Guo, J.Zhang, Y.Li, H.Chu, H.Wang, Q.Jiang, S.Li, Y.Shen, H.Li, G.Chen, J.Xu, C.

(2018) PLoS Biol 16: e2006525-e2006525

  • DOI: https://doi.org/10.1371/journal.pbio.2006525
  • Primary Citation of Related Structures:  
    5ZAZ

  • PubMed Abstract: 

    Protein transmembrane domains (TMDs) are generally hydrophobic, but our bioinformatics analysis shows that many TMDs contain basic residues at terminal regions. Physiological functions of these membrane-snorkeling basic residues are largely unclear. Here, we show that a membrane-snorkeling Lys residue in integrin αLβ2 (also known as lymphocyte function-associated antigen 1 [LFA-1]) regulates transmembrane heterodimer formation and integrin adhesion through ionic interplay with acidic phospholipids and calcium ions (Ca2+) in T cells. The amino group of the conserved Lys ionically interacts with the phosphate group of acidic phospholipids to stabilize αLβ2 transmembrane association, thus keeping the integrin at low-affinity conformation. Intracellular Ca2+ uses its charge to directly disrupt this ionic interaction, leading to the transmembrane separation and the subsequent extracellular domain extension to increase adhesion activity. This Ca2+-mediated regulation is independent on the canonical Ca2+ signaling or integrin inside-out signaling. Our work therefore showcases the importance of intramembrane ionic protein-lipid interaction and provides a new mechanism of integrin activation.


  • Organizational Affiliation

    State Key Laboratory of Molecular Biology, Shanghai Science Research Center, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Integrin beta-252Homo sapiensMutation(s): 1 
Gene Names: ITGB2CD18MFI7
Membrane Entity: Yes 
UniProt & NIH Common Fund Data Resources
Find proteins for P05107 (Homo sapiens)
Explore P05107 
Go to UniProtKB:  P05107
PHAROS:  P05107
GTEx:  ENSG00000160255 
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupP05107
Sequence Annotations
Expand
  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: SOLUTION NMR
  • Conformers Calculated: 100 
  • Conformers Submitted: 20 
  • Selection Criteria: target function 

Structure Validation

View Full Validation Report



Entry History & Funding Information

Deposition Data


Funding OrganizationLocationGrant Number
NSFCChina31470734
NSFCChina31670751
MOSTChina2014CB541903
CAS Key Research Program of Frontier SciencesChinaQYZDB-SSW-SMC048
CAS Facility-based Open Research ProgramChina--
NSFC Innovative Research GroupChina31621003
NSFC Key ProgramChina31530022
Ten Thousand Talent Program National Program for Support of Top-notch Young Professionals of ChinaChina--
STSMC Key ProgramChina16JC1404800
NSFC National Science Fund for Distinguished Young ScholarsChina31425009
STSMC Outstanding Academic Leader ProgramChina15XD1504200
CAS Strategic Priority Research ProgramChinaXDB08020100

Revision History  (Full details and data files)

  • Version 1.0: 2018-10-17
    Type: Initial release
  • Version 1.1: 2018-11-28
    Changes: Data collection, Database references
  • Version 1.2: 2023-06-14
    Changes: Data collection, Database references, Other
  • Version 1.3: 2024-05-15
    Changes: Data collection, Database references