5AXU

Crystal Structure of Cypovirus Polyhedra R13A Mutant

Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 1.60 Å
  • R-Value Free: 0.189 
  • R-Value Work: 0.160 
  • R-Value Observed: 0.162 

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Ligand Structure Quality Assessment 


This is version 1.3 of the entry. See complete history. 


Literature

Design of Enzyme-Encapsulated Protein Containers by In Vivo Crystal Engineering

Abe, S., Ijiri, H., Negishi, H., Yamanaka, H., Sasaki, K., Hirata, K., Mori, H., Ueno, T.

(2015) Adv Mater 27: 7951-7956

  • DOI: https://doi.org/10.1002/adma.201503827
  • Primary Citation of Related Structures:  
    5AXU, 5AXV

  • PubMed Abstract: 

    Crystalline protein assemblies of polyhedra crystal (PhC) can be utilized as solid enzyme containers for long-term storage of enzymes with retention of their enzymatic activity. The enzymes can be released from the crystals at the optimum pH for the enzymatic activity by dissolution of the crystals using in vivo crystal engineering.

    • Organizational Affiliation: 

    Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta-cho, 4259-B55, Midori-ku, Yokohama, 226-8501, Japan.


Macromolecules
Find similar proteins by: 
(by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Polyhedrin248Bombyx mori cypovirus 1Mutation(s): 2 
UniProt
Find proteins for P11041 (Bombyx mori cytoplasmic polyhedrosis virus)
Explore P11041 
Go to UniProtKB:  P11041
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupP11041
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 1.60 Å
  • R-Value Free: 0.189 
  • R-Value Work: 0.160 
  • R-Value Observed: 0.162 
  • Space Group: I 2 3
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 103.229α = 90
b = 103.229β = 90
c = 103.229γ = 90
Software Package:
Software NamePurpose
SCALEPACKdata scaling
MOLREPphasing
REFMACrefinement

Structure Validation

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Ligand Structure Quality Assessment 


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Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2015-11-11
    Type: Initial release
  • Version 1.1: 2016-01-27
    Changes: Database references
  • Version 1.2: 2020-02-26
    Changes: Data collection, Derived calculations
  • Version 1.3: 2023-11-08
    Changes: Data collection, Database references, Refinement description