1H3Z

Solution structure of a PWWP domain from Schizosaccharomyces Pombe


Experimental Data Snapshot

  • Method: SOLUTION NMR
  • Conformers Calculated: 30 
  • Conformers Submitted: 23 
  • Selection Criteria: NO VIOLATION GREATER THAN 0.25A AND NO VIOLATIONS GREATER THAN 5 DEGREES 

wwPDB Validation   3D Report Full Report


This is version 1.5 of the entry. See complete history


Literature

Structural Variation in Pwwp Domains

Slater, L.M.Allen, M.D.Bycroft, M.

(2003) J Mol Biol 330: 571-576

  • DOI: https://doi.org/10.1016/s0022-2836(03)00470-4
  • Primary Citation of Related Structures:  
    1H3Z

  • PubMed Abstract: 

    The PWWP domain is a ubiquitous eukaryotic protein module characterised by a region of sequence similarity of approximately 80 amino acids containing a highly conserved PWWP motif. It is frequently found in proteins associated with chromatin. We have determined the structure of a PWWP domain from the S. pombe protein SPBC215.07c using NMR spectroscopy. The structure is composed of a five stranded beta barrel followed by two alpha helices. Comparison to the recently reported structure of a homologous domain from the mammalian DNA methyltransferase Dnmt3b reveals substantial differences both in the C-terminal helical region and in the PWWP motif.


  • Organizational Affiliation

    MRC Centre for Protein Engineering, Hills Road, Cambridge CB2 2QH, UK.


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
HYPOTHETICAL 62.8 KDA PROTEIN C215.07C109Schizosaccharomyces pombeMutation(s): 0 
UniProt
Find proteins for O94312 (Schizosaccharomyces pombe (strain 972 / ATCC 24843))
Explore O94312 
Go to UniProtKB:  O94312
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupO94312
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: SOLUTION NMR
  • Conformers Calculated: 30 
  • Conformers Submitted: 23 
  • Selection Criteria: NO VIOLATION GREATER THAN 0.25A AND NO VIOLATIONS GREATER THAN 5 DEGREES 

Structure Validation

View Full Validation Report



Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2003-07-10
    Type: Initial release
  • Version 1.1: 2011-05-08
    Changes: Version format compliance
  • Version 1.2: 2011-07-13
    Changes: Version format compliance
  • Version 1.3: 2018-01-17
    Changes: Database references
  • Version 1.4: 2018-01-24
    Changes: Source and taxonomy
  • Version 1.5: 2024-05-15
    Changes: Data collection, Database references, Other