Download MendeleyCrystal structure of WhiB6 and SigA4-betaTip complex
Wan, T., Zhang, L.M.To be published.
8D5V
WhiB6 bound to the SigmaAr4-RNAP Beta flap tip chimera
- PDB DOI: https://doi.org/10.2210/pdb8D5V/pdb
- Classification: TRANSCRIPTION/Transferase
- Organism(s): Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
- Expression System: Escherichia coli
- Mutation(s): No
- Deposited: 2022-06-06 Released: 2023-06-14
- Funding Organization(s): National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS), National Science Foundation (NSF, United States)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.80 Å
- R-Value Free: 0.231
- R-Value Work: 0.210
- R-Value Observed: 0.211
wwPDB Validation 3D Report Full Report
This is version 1.1 of the entry. See complete history.
Literature
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Probable transcriptional regulator WhiB6 | 116 | Mycobacterium tuberculosis | Mutation(s): 0 Gene Names: whiB6, Rv3862c |  | |
UniProt | |||||
Find proteins for P9WF37 (Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)) Explore P9WF37 Go to UniProtKB: P9WF37 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P9WF37 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| RNA polymerase sigma factor SigA,DNA-directed RNA polymerase subunit beta | 112 | Mycobacterium tuberculosis H37Rv, Mycobacterium tuberculosis This entity is chimeric | Mutation(s): 0 Gene Names: sigA, mysA, rpoD, rpoV, Rv2703, MTCY05A6.24, rpoB, Rv0667, MTCI376.08c EC: 2.7.7.6 |  | |
UniProt | |||||
Find proteins for P9WGY9 (Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)) Explore P9WGY9 Go to UniProtKB: P9WGY9 | |||||
Find proteins for P9WGI1 (Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)) Explore P9WGI1 Go to UniProtKB: P9WGI1 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Groups | P9WGY9P9WGI1 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| SF4 (Subject of Investigation/LOI) Query on SF4 | E [auth A], F [auth C] | IRON/SULFUR CLUSTER Fe4 S4 LJBDFODJNLIPKO-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.80 Å
- R-Value Free: 0.231
- R-Value Work: 0.210
- R-Value Observed: 0.211
- Space Group: C 2 2 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 62.983 | α = 90 |
| b = 66.54 | β = 90 |
| c = 158.805 | γ = 90 |
| Software Name | Purpose |
|---|---|
| PHENIX | refinement |
| XDS | data reduction |
| SCALA | data scaling |
| PHASER | phasing |
Entry History & Funding Information
Deposition Data
- Released Date: 2023-06-14 Deposition Author(s): Wan, T., Zhang, L.M.
| Funding Organization | Location | Grant Number |
|---|---|---|
| National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) | United States | R35 GM138157-01 |
| National Science Foundation (NSF, United States) | United States | CLP 1846908 |
Revision History (Full details and data files)
- Version 1.0: 2023-06-14
Type: Initial release - Version 1.1: 2023-10-25
Changes: Data collection, Refinement description
