7YH8

Crystal structure of a heterochiral protein complex

PDB DOI: https://doi.org/10.2210/pdb7YH8/pdb

Classification: DE NOVO PROTEIN
Organism(s): synthetic construct, artificial sequences
Expression System: Escherichia coli
Mutation(s): No

Deposited: 2022-07-13 Released: 2023-07-19
Deposition Author(s): Liang, M., Li, S., Wang, T., Liu, L., Lu, P.
Funding Organization(s): National Natural Science Foundation of China (NSFC), Ministry of Science and Technology (MoST, China)

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.20 Å
R-Value Free: 0.250
R-Value Work: 0.202
R-Value Observed: 0.205

wwPDB Validation 3D Report Full Report

This is version 1.0 of the entry. See complete history.

Literature

Crystal structure of a heterochiral protein complex

Liu, L., Lu, P.

To be published.

Macromolecule Content

Total Structure Weight: 19.37 kDa
Atom Count: 1,440
Modelled Residue Count: 162
Deposited Residue Count: 162
Unique protein chains: 2

Macromolecules

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(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
L-19437	A, C	62	synthetic construct	Mutation(s): 0
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
Sequence Annotations Expand
Reference Sequence

Find similar proteins by: Sequence | 3D Structure

Entity ID: 2
Molecule	Chains	Sequence Length	Organism	Details	Image
D-Pep-1	B, D	19	artificial sequences	Mutation(s): 0
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.20 Å
R-Value Free: 0.250
R-Value Work: 0.202
R-Value Observed: 0.205
Space Group: P 2₁ 2₁ 2

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 54.064	α = 90
b = 81.922	β = 90
c = 35.722	γ = 90

Software Package:

Software Name	Purpose
PHENIX	refinement
HKL-2000	data scaling
Coot	model building
HKL-2000	data reduction
PHASER	phasing

Structure Validation

View Full Validation Report

Entry History & Funding Information

Deposition Data

Released Date: 2023-07-19

Deposition Author(s):

Funding Organization	Location	Grant Number
National Natural Science Foundation of China (NSFC)	China	22137005
Ministry of Science and Technology (MoST, China)	China	2020YFA0909200

Revision History (Full details and data files)

Version 1.0: 2023-07-19
Type: Initial release

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.