Download MendeleyMolecular characterization of a single stranded DNA binding protein from Fervidobacterium gondwanense
Werbowy, O., Hakansson, M., Svensson, L.A., Al-Karadaghi, S., Kaczorowski, T., Kaczarowska, A.K., Dorawa, S.To be published.
7R4W
Single stranded DNA binding protein SSB M5 from Fervidobacterium gondwanense
- PDB DOI: https://doi.org/10.2210/pdb7R4W/pdb
- Classification: DNA BINDING PROTEIN
- Organism(s): Fervidobacterium gondwanense
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2022-02-09 Released: 2023-08-23
- Funding Organization(s): European Research Council (ERC)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.30 Å
- R-Value Free: 0.255
- R-Value Work: 0.187
- R-Value Observed: 0.190
This is version 1.0 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Single-stranded DNA-binding protein | 145 | Fervidobacterium gondwanense | Mutation(s): 0 Gene Names: SAMN02745226_00543 |  | |
UniProt | |||||
Find proteins for A0A1M7S684 (Fervidobacterium gondwanense DSM 13020) Explore A0A1M7S684 Go to UniProtKB: A0A1M7S684 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | A0A1M7S684 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| PO4 (Subject of Investigation/LOI) Query on PO4 | D [auth A] | PHOSPHATE ION O4 P NBIIXXVUZAFLBC-UHFFFAOYSA-K |  | ||
| ACY Query on ACY | B [auth A], C [auth A] | ACETIC ACID C2 H4 O2 QTBSBXVTEAMEQO-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.30 Å
- R-Value Free: 0.255
- R-Value Work: 0.187
- R-Value Observed: 0.190
- Space Group: I 41 2 2
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 62.39 | α = 90 |
| b = 62.39 | β = 90 |
| c = 172.38 | γ = 90 |
| Software Name | Purpose |
|---|---|
| XDS | data reduction |
| Aimless | data scaling |
| REFMAC | refinement |
| PDB_EXTRACT | data extraction |
| MrBUMP | phasing |
Entry History & Funding Information
Deposition Data
- Released Date: 2023-08-23 Deposition Author(s): Hakansson, M., Svensson, L.A., Werbowy, O., Al-Karadaghi, S., Kaczorowski, T., Kaczorowska, A.K., Dorawa, S.
| Funding Organization | Location | Grant Number |
|---|---|---|
| European Research Council (ERC) | European Union | 685778 |
Revision History (Full details and data files)
- Version 1.0: 2023-08-23
Type: Initial release
