7QIH

Complex of the Yersinia enterocolitica Type III secretion proteins YscX and YscY


Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 1.92 Å
  • R-Value Free: 0.262 
  • R-Value Work: 0.221 
  • R-Value Observed: 0.223 

wwPDB Validation   3D Report Full Report


This is version 1.1 of the entry. See complete history


Literature

Direct interaction of a chaperone-bound type III secretion substrate with the export gate.

Gilzer, D.Schreiner, M.Niemann, H.H.

(2022) Nat Commun 13: 2858-2858

  • DOI: https://doi.org/10.1038/s41467-022-30487-1
  • Primary Citation of Related Structures:  
    7QIH, 7QII, 7QIJ

  • PubMed Abstract: 

    Several gram-negative bacteria employ type III secretion systems (T3SS) to inject effector proteins into eukaryotic host cells directly from the bacterial cytoplasm. The export gate SctV (YscV in Yersinia) binds substrate:chaperone complexes such as YscX:YscY, which are essential for formation of a functional T3SS. Here, we present structures of the YscX:YscY complex alone and bound to nonameric YscV. YscX binds its chaperone YscY at two distinct sites, resembling the heterotrimeric complex of the T3SS needle subunit with its chaperone and co-chaperone. In the ternary complex the YscX N-terminus, which mediates YscX secretion, occupies a binding site within one YscV that is also used by flagellar chaperones, suggesting the interaction's importance for substrate recognition. The YscX C-terminus inserts between protomers of the YscV ring where the stalk protein binds to couple YscV to the T3SS ATPase. This primary YscV-YscX interaction is essential for the formation of a secretion-competent T3SS.


  • Organizational Affiliation

    Department of Chemistry, Bielefeld University, Universitaetstrasse 25, 33615, Bielefeld, Germany.


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Chaperone protein YscY
A, C
122Yersinia enterocoliticaMutation(s): 0 
Gene Names: yscY
UniProt
Find proteins for P0C2N2 (Yersinia enterocolitica)
Explore P0C2N2 
Go to UniProtKB:  P0C2N2
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupP0C2N2
Sequence Annotations
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  • Reference Sequence
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 2
MoleculeChains Sequence LengthOrganismDetailsImage
Yop proteins translocation protein X
B, D
77Yersinia enterocoliticaMutation(s): 0 
Gene Names: yscX
UniProt
Find proteins for P0C2N4 (Yersinia enterocolitica)
Explore P0C2N4 
Go to UniProtKB:  P0C2N4
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupP0C2N4
Sequence Annotations
Expand
  • Reference Sequence
Experimental Data & Validation

Experimental Data

Unit Cell:
Length ( Å )Angle ( ˚ )
a = 41.553α = 90
b = 140.833β = 90
c = 59.88γ = 90
Software Package:
Software NamePurpose
PHENIXrefinement
XDSdata reduction
PHASERphasing
BUCCANEERmodel building
XDSdata scaling

Structure Validation

View Full Validation Report



Entry History & Funding Information

Deposition Data


Funding OrganizationLocationGrant Number
Not funded--

Revision History  (Full details and data files)

  • Version 1.0: 2022-06-01
    Type: Initial release
  • Version 1.1: 2022-06-15
    Changes: Database references