Download MendeleyCryoEM analysis of small plant biocatalysts at sub-2 angstrom resolution.
Dimos, N., Helmer, C.P.O., Chanique, A.M., Wahl, M.C., Kourist, R., Hilal, T., Loll, B.(2022) Acta Crystallogr D Struct Biol 78: 113-123
- PubMed: 34981767
- DOI: https://doi.org/10.1107/S205979832101216X
- Primary Citation of Related Structures:
7O6P, 7O6Q - PubMed Abstract:
Enzyme catalysis has emerged as a key technology for developing efficient, sustainable processes in the chemical, biotechnological and pharmaceutical industries. Plants provide large and diverse pools of biosynthetic enzymes that facilitate complex reactions, such as the formation of intricate terpene carbon skeletons, with exquisite specificity. High-resolution structural analysis of these enzymes is crucial in order to understand their mechanisms and modulate their properties by targeted engineering. Although cryo-electron microscopy (cryoEM) has revolutionized structural biology, its applicability to high-resolution structural analysis of comparatively small enzymes has so far been largely unexplored. Here, it is shown that cryoEM can reveal the structures of plant borneol dehydrogenases of ∼120 kDa at or below 2 Å resolution, paving the way for the rapid development of new biocatalysts that can provide access to bioactive terpenes and terpenoids.
Organizational Affiliation:
Institute of Chemistry and Biochemistry, Department of Biology, Chemistry, Pharmacy, Laboratory of Structural Biochemistry, Free University of Berlin, Takustrasse 6, 14195 Berlin, Germany.
