7BQA

Crystal structure of ASFV p35

PDB DOI: https://doi.org/10.2210/pdb7BQA/pdb

Classification: STRUCTURAL PROTEIN
Organism(s): African swine fever virus
Expression System: Drosophila melanogaster
Mutation(s): No

Deposited: 2020-03-24 Released: 2020-06-24
Deposition Author(s): Li, G.B., Fu, D., Chen, C., Guo, Y.
Funding Organization(s): National Natural Science Foundation of China (NSFC)

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.10 Å
R-Value Free: 0.228
R-Value Work: 0.200
R-Value Observed: 0.203

wwPDB Validation 3D Report Full Report

This is version 1.1 of the entry. See complete history.

Literature

Crystal structure of the African swine fever virus structural protein p35 reveals its role for core shell assembly.

Li, G., Fu, D., Zhang, G., Zhao, D., Li, M., Geng, X., Sun, D., Wang, Y., Chen, C., Jiao, P., Cao, L., Guo, Y., Rao, Z.

(2020) Protein Cell 11: 600-605

PubMed: 32519301 Search on PubMedSearch on PubMed Central
DOI: https://doi.org/10.1007/s13238-020-00730-w
Primary Citation of Related Structures:
7BQA

Organizational Affiliation

State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin, 300350, China.

Macromolecule Content

Total Structure Weight: 78.09 kDa
Atom Count: 4,663
Modelled Residue Count: 536
Deposited Residue Count: 678
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
60 kDa polyprotein	A [auth B], B [auth A]	339	African swine fever virus	Mutation(s): 0 Gene Names: CP530R, AFSV47Ss_0139
UniProt
Find proteins for Q65179 (African swine fever virus (strain Badajoz 1971 Vero-adapted)) Explore Q65179 Go to UniProtKB: Q65179
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q65179
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.10 Å
R-Value Free: 0.228
R-Value Work: 0.200
R-Value Observed: 0.203
Space Group: P 1 2₁ 1

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 58.068	α = 90
b = 89.563	β = 89.95
c = 65.08	γ = 90

Software Package:

Software Name	Purpose
HKL-2000	data scaling
PHENIX	refinement
PDB_EXTRACT	data extraction
HKL-3000	data reduction
PHASER	phasing

Structure Validation

View Full Validation Report

Entry History & Funding Information

Deposition Data

Released Date: 2020-06-24

Deposition Author(s):

Funding Organization	Location	Grant Number
National Natural Science Foundation of China (NSFC)	China	31670731
National Natural Science Foundation of China (NSFC)	China	31870733
National Natural Science Foundation of China (NSFC)	China	31941011

Revision History (Full details and data files)

Version 1.0: 2020-06-24
Type: Initial release
Version 1.1: 2021-05-05
Changes: Database references

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.