7P0H

Crystal structure of Helicobacter pylori ComF fused to an artificial alphaREP crystallization helper(named B2)

PDB DOI: https://doi.org/10.2210/pdb7P0H/pdb

Classification: RECOMBINATION
Organism(s): synthetic construct, Helicobacter pylori 26695
Expression System: Escherichia coli 'BL21-Gold(DE3)pLysS AG
Mutation(s): No

Deposited: 2021-06-29 Released: 2022-04-06
Deposition Author(s): Celma, L., Walbott, H., Legrand, P., Quevillon-Cheruel, S.
Funding Organization(s): French Infrastructure for Integrated Structural Biology (FRISBI)

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.50 Å
R-Value Free: 0.245
R-Value Work: 0.221
R-Value Observed: 0.222

wwPDB Validation 3D Report Full Report

Ligand Structure Quality Assessment

This is version 1.1 of the entry. See complete history.

Macromolecule Content

Total Structure Weight: 191.79 kDa
Atom Count: 13,138
Modelled Residue Count: 1,644
Deposited Residue Count: 1,708
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Helicobacter pylori ComF fused to an artificial alphaREP crystallization helper (named B2)	A, B, C, D	427	synthetic construct, Helicobacter pylori 26695 This entity is chimeric	Mutation(s): 0
UniProt
Find proteins for O26008 (Helicobacter pylori (strain ATCC 700392 / 26695)) Explore O26008 Go to UniProtKB: O26008
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	O26008
Sequence Annotations Expand
Reference Sequence

Small Molecules

Ligands 4 Unique
ID	Chains	Name / Formula / InChI Key	2D Diagram	3D Interactions
PRP (Subject of Investigation/LOI) Query on PRP Download Ideal Coordinates CCD File SDF format, chain DA [auth D] SDF format, chain F [auth A] SDF format, chain P [auth B] SDF format, chain V [auth C] MOL2 format, chain DA [auth D] MOL2 format, chain F [auth A] MOL2 format, chain P [auth B] MOL2 format, chain V [auth C]	DA [auth D], F [auth A], P [auth B], V [auth C]	1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose C₅ H₁₃ O₁₄ P₃ PQGCEDQWHSBAJP-TXICZTDVSA-N		Interactions Focus chain DA [auth D] Focus chain F [auth A] Focus chain P [auth B] Focus chain V [auth C] Interactions & Density Focus chain DA [auth D] Focus chain F [auth A] Focus chain P [auth B] Focus chain V [auth C]
GOL Query on GOL Download Ideal Coordinates CCD File SDF format, chain FA [auth D] SDF format, chain GA [auth D] SDF format, chain H [auth A] SDF format, chain I [auth A] SDF format, chain J [auth A] SDF format, chain K [auth A] SDF format, chain L [auth A] SDF format, chain M [auth A] SDF format, chain R [auth B] SDF format, chain S [auth B] SDF format, chain Y [auth C] SDF format, chain Z [auth C] SDF format, chain AA [auth C] SDF format, chain X [auth C] MOL2 format, chain FA [auth D] MOL2 format, chain GA [auth D] MOL2 format, chain H [auth A] MOL2 format, chain I [auth A] MOL2 format, chain J [auth A] MOL2 format, chain K [auth A] MOL2 format, chain L [auth A] MOL2 format, chain M [auth A] MOL2 format, chain R [auth B] MOL2 format, chain S [auth B] MOL2 format, chain Y [auth C] MOL2 format, chain Z [auth C] MOL2 format, chain AA [auth C] MOL2 format, chain X [auth C]	AA [auth C] FA [auth D] GA [auth D] H [auth A] I [auth A] AA [auth C], FA [auth D], GA [auth D], H [auth A], I [auth A], J [auth A], K [auth A], L [auth A], M [auth A], R [auth B], S [auth B], X [auth C], Y [auth C], Z [auth C]	GLYCEROL C₃ H₈ O₃ PEDCQBHIVMGVHV-UHFFFAOYSA-N		Interactions Focus chain AA [auth C] Focus chain FA [auth D] Focus chain GA [auth D] Focus chain H [auth A] Focus chain I [auth A] Focus chain J [auth A] Focus chain K [auth A] Focus chain L [auth A] Focus chain M [auth A] Focus chain R [auth B] Focus chain S [auth B] Focus chain X [auth C] Focus chain Y [auth C] Focus chain Z [auth C] Interactions & Density Focus chain AA [auth C] Focus chain FA [auth D] Focus chain GA [auth D] Focus chain H [auth A] Focus chain I [auth A] Focus chain J [auth A] Focus chain K [auth A] Focus chain L [auth A] Focus chain M [auth A] Focus chain R [auth B] Focus chain S [auth B] Focus chain X [auth C] Focus chain Y [auth C] Focus chain Z [auth C]
ZN (Subject of Investigation/LOI) Query on ZN Download Ideal Coordinates CCD File SDF format, chain E [auth A] SDF format, chain U [auth C] SDF format, chain CA [auth D] SDF format, chain O [auth B] MOL2 format, chain E [auth A] MOL2 format, chain U [auth C] MOL2 format, chain CA [auth D] MOL2 format, chain O [auth B]	CA [auth D], E [auth A], O [auth B], U [auth C]	ZINC ION Zn PTFCDOFLOPIGGS-UHFFFAOYSA-N		Interactions Focus chain CA [auth D] Focus chain E [auth A] Focus chain O [auth B] Focus chain U [auth C] Interactions & Density Focus chain CA [auth D] Focus chain E [auth A] Focus chain O [auth B] Focus chain U [auth C]
MG (Subject of Investigation/LOI) Query on MG Download Ideal Coordinates CCD File SDF format, chain BA [auth C] SDF format, chain EA [auth D] SDF format, chain G [auth A] SDF format, chain HA [auth D] SDF format, chain N [auth A] SDF format, chain Q [auth B] SDF format, chain T [auth B] SDF format, chain W [auth C] MOL2 format, chain BA [auth C] MOL2 format, chain EA [auth D] MOL2 format, chain G [auth A] MOL2 format, chain HA [auth D] MOL2 format, chain N [auth A] MOL2 format, chain Q [auth B] MOL2 format, chain T [auth B] MOL2 format, chain W [auth C]	BA [auth C] EA [auth D] G [auth A] HA [auth D] N [auth A] BA [auth C], EA [auth D], G [auth A], HA [auth D], N [auth A], Q [auth B], T [auth B], W [auth C]	MAGNESIUM ION Mg JLVVSXFLKOJNIY-UHFFFAOYSA-N		Interactions Focus chain BA [auth C] Focus chain EA [auth D] Focus chain G [auth A] Focus chain HA [auth D] Focus chain N [auth A] Focus chain Q [auth B] Focus chain T [auth B] Focus chain W [auth C] Interactions & Density Focus chain BA [auth C] Focus chain EA [auth D] Focus chain G [auth A] Focus chain HA [auth D] Focus chain N [auth A] Focus chain Q [auth B] Focus chain T [auth B] Focus chain W [auth C]