Download MendeleyCrystal structure of the human METTL3-METTL14 complex bound to Compound 12 (ADO_AD_066)
Bedi, R.K., Dolbois, A., Caflisch, A.To be published.
7O0Q
Crystal structure of the human METTL3-METTL14 complex bound to Compound 12 (ADO_AD_066)
- PDB DOI: https://doi.org/10.2210/pdb7O0Q/pdb
- Classification: TRANSFERASE
- Organism(s): Homo sapiens
- Expression System: Spodoptera frugiperda
- Mutation(s): No
- Deposited: 2021-03-26 Released: 2021-09-15
- Funding Organization(s): Swiss National Science Foundation
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.49 Å
- R-Value Free: 0.278
- R-Value Work: 0.217
- R-Value Observed: 0.220
This is version 1.1 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| N6-adenosine-methyltransferase catalytic subunit | 246 | Homo sapiens | Mutation(s): 0 Gene Names: METTL3, MTA70 EC: 2.1.1.348 |  | |
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for Q86U44 (Homo sapiens) Explore Q86U44 Go to UniProtKB: Q86U44 | |||||
PHAROS: Q86U44 GTEx: ENSG00000165819 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q86U44 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| N6-adenosine-methyltransferase non-catalytic subunit | 290 | Homo sapiens | Mutation(s): 0 Gene Names: METTL14, KIAA1627 |  | |
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for Q9HCE5 (Homo sapiens) Explore Q9HCE5 Go to UniProtKB: Q9HCE5 | |||||
PHAROS: Q9HCE5 GTEx: ENSG00000145388 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q9HCE5 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| UY2 (Subject of Investigation/LOI) Query on UY2 | C [auth A] | 3-[4-[(4,4-dimethylpiperidin-1-yl)methyl]phenyl]-8-[6-(methylamino)pyrimidin-4-yl]-1,3,8-triazaspiro[4.5]decan-2-one C26 H37 N7 O JSLLBTAATPZHSC-UHFFFAOYSA-N |  | ||
| ACT Query on ACT | E [auth B] | ACETATE ION C2 H3 O2 QTBSBXVTEAMEQO-UHFFFAOYSA-M |  | ||
| MG Query on MG | D [auth B] | MAGNESIUM ION Mg JLVVSXFLKOJNIY-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.49 Å
- R-Value Free: 0.278
- R-Value Work: 0.217
- R-Value Observed: 0.220
- Space Group: P 32 2 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 64.17 | α = 90 |
| b = 64.17 | β = 90 |
| c = 225.71 | γ = 120 |
| Software Name | Purpose |
|---|---|
| XDS | data reduction |
| XSCALE | data scaling |
| PHASER | phasing |
| PHENIX | refinement |
Entry History & Funding Information
Deposition Data
- Released Date: 2021-09-15 Deposition Author(s): Bedi, R.K., Dolbois, A., Caflisch, A.
| Funding Organization | Location | Grant Number |
|---|---|---|
| Swiss National Science Foundation | Switzerland | 310030B_189363 |
Revision History (Full details and data files)
- Version 1.0: 2021-09-15
Type: Initial release - Version 1.1: 2024-01-31
Changes: Data collection, Refinement description
