6VE6

A structural characterization of poly(aspartic acid) hydrolase-1 from Sphingomonas sp. KT-1.

PDB DOI: https://doi.org/10.2210/pdb6VE6/pdb

Classification: HYDROLASE
Organism(s): Sphingomonas sp. KT-1
Expression System: Sphingomonas sp. KT-1
Mutation(s): No

Deposited: 2019-12-28 Released: 2020-12-09
Deposition Author(s): Bolay, A.L., Salvo, H., Brambley, C.A., Yared, T.J., Miller, J.M., Wallen, J.R., Weiland, M.H.
Funding Organization(s): National Science Foundation (NSF, United States)

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.45 Å
R-Value Free: 0.290
R-Value Work: 0.236
R-Value Observed: 0.238

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This is version 1.1 of the entry. See complete history.

Literature

Structural Characterization of Sphingomonas sp. KT-1 PahZ1-Catalyzed Biodegradation of Thermally Synthesized Poly(aspartic acid)

Brambley, C.A., Bolay, A.L., Salvo, H., Jansch, A.L., Yared, T.J., Miller, J.M., Wallen, J.R., Weiland, M.H.

(2020) ACS Sustain Chem Eng

DOI: https://doi.org/10.1021/acssuschemeng.0c01158

Macromolecule Content

Total Structure Weight: 131.1 kDa
Atom Count: 8,833
Modelled Residue Count: 1,062
Deposited Residue Count: 1,180
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Poly(Aspartic acid) hydrolase-1	A, B, C, D	295	Sphingomonas sp. KT-1	Mutation(s): 0 Gene Names: pahZ
UniProt
Find proteins for Q7WSC1 (Sphingomonas sp. KT-1) Explore Q7WSC1 Go to UniProtKB: Q7WSC1
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q7WSC1
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.45 Å
R-Value Free: 0.290
R-Value Work: 0.236
R-Value Observed: 0.238
Space Group: P 2₁ 2₁ 2₁

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 81.749	α = 90
b = 87.006	β = 90
c = 171.06	γ = 90

Software Package:

Software Name	Purpose
HKL-3000	data scaling
PHASER	phasing
PHENIX	refinement
PDB_EXTRACT	data extraction
HKL-3000	data reduction

Structure Validation

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Entry History & Funding Information

Deposition Data

Released Date: 2020-12-09

Deposition Author(s):

Funding Organization	Location	Grant Number
National Science Foundation (NSF, United States)	United States	DUE 1611988

Revision History (Full details and data files)

Version 1.0: 2020-12-09
Type: Initial release
Version 1.1: 2023-10-11
Changes: Data collection, Database references, Refinement description

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.