6VBW

Cryo-EM structure of Cascade-TniQ-dsDNA ternary complex

Experimental Data Snapshot

  • Method: ELECTRON MICROSCOPY
  • Resolution: 3.20 Å
  • Aggregation State: PARTICLE 
  • Reconstruction Method: SINGLE PARTICLE 

wwPDB Validation   3D Report Full Report


This is version 1.2 of the entry. See complete history. 


Literature

Structure-function insights into the initial step of DNA integration by a CRISPR-Cas-Transposon complex.

Jia, N., Xie, W., de la Cruz, M.J., Eng, E.T., Patel, D.J.

(2020) Cell Res 30: 182-184


Macromolecules

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Entity ID: 4
MoleculeChains Sequence LengthOrganismDetailsImage
Cas8D [auth A]640Vibrio choleraeMutation(s): 0 
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Entity ID: 5
MoleculeChains Sequence LengthOrganismDetailsImage
Cas7352Vibrio choleraeMutation(s): 0 
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Entity ID: 6
MoleculeChains Sequence LengthOrganismDetailsImage
Cas6K [auth H]199Vibrio choleraeMutation(s): 0 
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Entity ID: 7
MoleculeChains Sequence LengthOrganismDetailsImage
TniQL [auth I],
M [auth J]		394Vibrio choleraeMutation(s): 0 
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Entity ID: 1
MoleculeChains LengthOrganismImage
DNA (5'-D(*GP*CP*AP*GP*TP*CP*AP*TP*CP*AP*CP*CP*AP*AP*TP*TP*TP*AP*TP*TP*TP*A)-3')A [auth M]22Vibrio cholerae
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Entity ID: 2
MoleculeChains LengthOrganismImage
DNA (100-MER)B [auth L]100Vibrio cholerae
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Entity ID: 3
MoleculeChains LengthOrganismImage
RNA (61-MER)C [auth K]61Vibrio cholerae
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Experimental Data & Validation

Experimental Data

  • Method: ELECTRON MICROSCOPY
  • Resolution: 3.20 Å
  • Aggregation State: PARTICLE 
  • Reconstruction Method: SINGLE PARTICLE 
EM Software:
TaskSoftware PackageVersion
RECONSTRUCTIONRELION2.1

Structure Validation

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Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2020-01-29
    Type: Initial release
  • Version 1.1: 2020-02-19
    Changes: Database references
  • Version 1.2: 2024-03-06
    Changes: Data collection, Database references