6U7D

Recombinant stem bromelain precursor

PDB DOI: https://doi.org/10.2210/pdb6U7D/pdb

Classification: PLANT PROTEIN
Organism(s): Ananas comosus
Expression System: Escherichia coli BL21(DE3)
Mutation(s): No

Deposited: 2019-09-02 Released: 2019-09-18
Deposition Author(s): Yongqing, T., Pike, R.N., Wijeyewickrema, L.C.
Funding Organization(s): Australian SIEF-industrial fellowship fund

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 3.00 Å
R-Value Free: 0.314
R-Value Work: 0.198
R-Value Observed: 0.203

wwPDB Validation 3D Report Full Report

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This is version 1.1 of the entry. See complete history.

Literature

Crystal Structure of a Recombinant Stem Bromelain Precursor

Yongqing, T., Pike, R.N., Wijeyewickrema, L.C.

To be published.

Macromolecule Content

Total Structure Weight: 36.87 kDa
Atom Count: 2,354
Modelled Residue Count: 302
Deposited Residue Count: 332
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
FBSB	A	332	Ananas comosus	Mutation(s): 0
UniProt
Find proteins for A0A199VSS3 (Ananas comosus) Explore A0A199VSS3 Go to UniProtKB: A0A199VSS3
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	A0A199VSS3
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 3.00 Å
R-Value Free: 0.314
R-Value Work: 0.198
R-Value Observed: 0.203
Space Group: P 2 2₁ 2₁

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 64.31	α = 90
b = 69.94	β = 90
c = 74.25	γ = 90

Software Package:

Software Name	Purpose
REFMAC	refinement
PDB_EXTRACT	data extraction
iMOSFLM	data reduction
SCALA	data scaling
PHENIX	phasing

Structure Validation

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Entry History & Funding Information

Deposition Data

Released Date: 2019-09-18

Deposition Author(s):

Wijeyewickrema, L.C.

Funding Organization	Location	Grant Number
Australian SIEF-industrial fellowship fund	Australia	--

Revision History (Full details and data files)

Version 1.0: 2019-09-18
Type: Initial release
Version 1.1: 2023-10-11
Changes: Data collection, Database references, Refinement description

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.