Download MendeleyStructural basis of elongation factor 2 switching
Fenwick, M.K., Ealick, S.E.(2020) Curr Res Struct Biol 2: 25-34
6U45
Crystal structure of Methanoperedens nitroreducens elongation factor 2 bound to GMPPCP and magnesium
- PDB DOI: https://doi.org/10.2210/pdb6U45/pdb
- Classification: TRANSLATION
- Organism(s): Candidatus Methanoperedens nitroreducens
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2019-08-22 Released: 2020-03-18
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.35 Å
- R-Value Free: 0.224
- R-Value Work: 0.168
- R-Value Observed: 0.171
This is version 1.2 of the entry. See complete history.
Literature
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Elongation factor 2 | 732 | Candidatus Methanoperedens nitroreducens | Mutation(s): 0 Gene Names: fusA, ANME2D_00299 |  | |
UniProt | |||||
Find proteins for A0A062V290 (Candidatus Methanoperedens nitroreducens) Explore A0A062V290 Go to UniProtKB: A0A062V290 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | A0A062V290 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| GCP Query on GCP | C [auth A], F [auth B] | PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER C11 H18 N5 O13 P3 PHBDHXOBFUBCJD-KQYNXXCUSA-N |  | ||
| CL Query on CL | E [auth A], H [auth B] | CHLORIDE ION Cl VEXZGXHMUGYJMC-UHFFFAOYSA-M |  | ||
| MG Query on MG | D [auth A], G [auth B] | MAGNESIUM ION Mg JLVVSXFLKOJNIY-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.35 Å
- R-Value Free: 0.224
- R-Value Work: 0.168
- R-Value Observed: 0.171
- Space Group: P 21 21 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 93.699 | α = 90 |
| b = 134.375 | β = 90 |
| c = 150.485 | γ = 90 |
| Software Name | Purpose |
|---|---|
| PHENIX | refinement |
| PDB_EXTRACT | data extraction |
| XDS | data reduction |
| Aimless | data scaling |
| PHASER | phasing |
Entry History
Deposition Data
- Released Date: 2020-03-18 Deposition Author(s): Fenwick, M.K., Ealick, S.E.
Revision History (Full details and data files)
- Version 1.0: 2020-03-18
Type: Initial release - Version 1.1: 2024-03-13
Changes: Data collection, Database references, Derived calculations - Version 1.2: 2024-04-03
Changes: Refinement description
