6TCI

The crystal structure of SleB N-terminal domain

PDB DOI: https://doi.org/10.2210/pdb6TCI/pdb

Classification: HYDROLASE
Organism(s): Bacillus cereus ATCC 14579
Expression System: Escherichia coli BL21(DE3)
Mutation(s): No

Deposited: 2019-11-06 Released: 2020-11-18
Deposition Author(s): Chirgadze, D.Y., Hardwick, S.W., Christie, G.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 1.47 Å
R-Value Free: 0.218
R-Value Work: 0.170
R-Value Observed: 0.174

wwPDB Validation 3D Report Full Report

This is version 1.1 of the entry. See complete history.

Re-refinement Note

This entry reflects an alternative modeling of the original data in: 4F55 4FET

Literature

The crystal structure of SleB N-terminal domain

Chirgadze, D.Y., Hardwick, S.W., Christie, G.

To be published.

Macromolecule Content

Total Structure Weight: 8.26 kDa
Atom Count: 633
Modelled Residue Count: 73
Deposited Residue Count: 74
Unique protein chains: 1

Macromolecules

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(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Spore cortex-lytic enzyme	A	74	Bacillus cereus ATCC 14579	Mutation(s): 0 Gene Names: sleB, BC_2753
UniProt
Find proteins for P0A3V0 (Bacillus cereus (strain ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NCTC 2599 / NRRL B-3711)) Explore P0A3V0 Go to UniProtKB: P0A3V0
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P0A3V0
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 1.47 Å
R-Value Free: 0.218
R-Value Work: 0.170
R-Value Observed: 0.174
Space Group: P 4₁ 2₁ 2

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 43.33	α = 90
b = 43.33	β = 90
c = 72.27	γ = 90

Software Package:

Software Name	Purpose
PHENIX	refinement
iMOSFLM	data reduction
Aimless	data scaling
PHASER	phasing

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2020-11-18

Deposition Author(s):

Chirgadze, D.Y.

Revision History (Full details and data files)

Version 1.0: 2020-11-18
Type: Initial release
Version 1.1: 2024-01-24
Changes: Data collection, Database references, Refinement description

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.