6RSX

Regulatory Subunit of cAMP-dependant Protein Kinase A from Euglena gracilis at 1.6 A resolution

PDB DOI: https://doi.org/10.2210/pdb6RSX/pdb

Classification: SIGNALING PROTEIN
Organism(s): Euglena gracilis
Expression System: Escherichia coli
Mutation(s): No

Deposited: 2019-05-22 Released: 2020-10-28
Deposition Author(s): Volpato Santos, Y., Ober, V., Basquin, J., Boshart, M.
Funding Organization(s): Brazilian National Council for Scientific and Technological Development

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 1.60 Å
R-Value Free: 0.216
R-Value Work: 0.192
R-Value Observed: 0.193

wwPDB Validation 3D Report Full Report

Ligand Structure Quality Assessment

This is version 1.2 of the entry. See complete history.

Literature

Regulatory Subunit of Protein Kinase A (cAMP-dependent) from Euglena gracilis at 1.6 A resolution

Volpato Santos, Y., Ober, V., Basquin, J., Boshart, M.

To be published.

Macromolecule Content

Total Structure Weight: 33.78 kDa
Atom Count: 2,384
Modelled Residue Count: 266
Deposited Residue Count: 293
Unique protein chains: 1

Macromolecules

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(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Regulatory Subunit of Protein Kinase A (cAMP-dependent) from Euglena gracilis	A	293	Euglena gracilis	Mutation(s): 0 Gene Names: EG_transcript_11236
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
Sequence Annotations Expand
Reference Sequence

Small Molecules

Ligands 1 Unique
ID	Chains	Name / Formula / InChI Key	2D Diagram	3D Interactions
CMP (Subject of Investigation/LOI) Query on CMP Download Ideal Coordinates CCD File SDF format, chain B [auth A] SDF format, chain C [auth A] MOL2 format, chain B [auth A] MOL2 format, chain C [auth A]	B [auth A], C [auth A]	ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE C₁₀ H₁₂ N₅ O₆ P IVOMOUWHDPKRLL-KQYNXXCUSA-N		Interactions Focus chain B [auth A] Focus chain C [auth A] Interactions & Density Focus chain B [auth A] Focus chain C [auth A]

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 1.60 Å
R-Value Free: 0.216
R-Value Work: 0.192
R-Value Observed: 0.193
Space Group: P 6₁

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 80.586	α = 90
b = 80.586	β = 90
c = 110.755	γ = 120

Software Package:

Software Name	Purpose
PHENIX	refinement
XDS	data reduction
PHASER	phasing
Aimless	data scaling
Coot	model building

Structure Validation

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Ligand Structure Quality Assessment

Entry History & Funding Information

Deposition Data

Released Date: 2020-10-28

Deposition Author(s):

Funding Organization	Location	Grant Number
Brazilian National Council for Scientific and Technological Development	Brazil	233134/2014-0

Revision History (Full details and data files)

Version 1.0: 2020-10-28
Type: Initial release
Version 1.1: 2020-11-11
Changes: Data collection
Version 1.2: 2024-01-24
Changes: Advisory, Data collection, Database references, Refinement description

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6RSX

Regulatory Subunit of cAMP-dependant Protein Kinase A from Euglena gracilis at 1.6 A resolution

Regulatory Subunit of Protein Kinase A (cAMP-dependent) from Euglena gracilis at 1.6 A resolution

Entity ID: 1

Entity Groups

Sequence Annotations

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Experimental Data

Structure Validation

Ligand Structure Quality Assessment

Deposition Data

Revision History (Full details and data files)