6KIL

N21Q mutant thioredoxin from Halobacterium salinarum NRC-1

PDB DOI: https://doi.org/10.2210/pdb6KIL/pdb

Classification: OXIDOREDUCTASE
Organism(s): Halobacterium salinarum NRC-1
Expression System: Escherichia coli
Mutation(s): Yes

Deposited: 2019-07-19 Released: 2020-01-15
Deposition Author(s): Arai, S., Shibazaki, C., Shimizu, R., Adachi, M., Ishibashi, M., Tokunaga, H., Tokunaga, M.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 1.60 Å
R-Value Free: 0.187
R-Value Work: 0.177
R-Value Observed: 0.177

wwPDB Validation 3D Report Full Report

This is version 1.1 of the entry. See complete history.

Literature

Catalytic mechanism and evolutional characteristics of thioredoxin from Halobacterium salinarum NRC-1.

Arai, S., Shibazaki, C., Shimizu, R., Adachi, M., Ishibashi, M., Tokunaga, H., Tokunaga, M.

(2020) Acta Crystallogr D Biol Crystallogr 76: 73-84

DOI: https://doi.org/10.1107/S2059798319015894

Macromolecule Content

Total Structure Weight: 13.19 kDa
Atom Count: 982
Modelled Residue Count: 113
Deposited Residue Count: 124
Unique protein chains: 1

Macromolecules

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(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Thioredoxin	A	124	Halobacterium salinarum NRC-1	Mutation(s): 1 Gene Names: trxA, trxA1_1, trxA1_2, VNG_6073G, VNG_6453G
UniProt
Find proteins for O46709 (Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)) Explore O46709 Go to UniProtKB: O46709
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	O46709
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 1.60 Å
R-Value Free: 0.187
R-Value Work: 0.177
R-Value Observed: 0.177
Space Group: P 2₁ 2₁ 2₁

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 40.917	α = 90
b = 43.283	β = 90
c = 54.435	γ = 90

Software Package:

Software Name	Purpose
PHENIX	refinement
HKL-2000	data reduction
HKL-2000	data scaling
PHASER	phasing

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2020-01-15

Deposition Author(s):

Revision History (Full details and data files)

Version 1.0: 2020-01-15
Type: Initial release
Version 1.1: 2023-11-22
Changes: Data collection, Database references, Refinement description

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.