6KET

Flavin-utilizing Monooxygenase (OX) Domain of Hybrid Polyketide/Non-Ribosomal Peptide Synthetase

PDB DOI: https://doi.org/10.2210/pdb6KET/pdb

Classification: OXIDOREDUCTASE
Organism(s): Cylindrospermum alatosporum CCALA 988
Expression System: Escherichia coli
Mutation(s): No

Deposited: 2019-07-05 Released: 2019-09-25
Deposition Author(s): Tian, Q.W., Jiang, T.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 1.60 Å
R-Value Free: 0.239
R-Value Work: 0.220
R-Value Observed: 0.221

wwPDB Validation 3D Report Full Report

This is version 1.1 of the entry. See complete history.

Literature

Structure Insights into the Molecular Mechanism of Two Tailoring Domains of Hybrid Polyketide/Non-Ribosomal Peptide Synthetase

Tian, Q.W., Jiang, T.

To be published.

Macromolecule Content

Total Structure Weight: 80.24 kDa
Atom Count: 5,734
Modelled Residue Count: 680
Deposited Residue Count: 724
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
PuwE	A, B	362	Cylindrospermum alatosporum CCALA 988	Mutation(s): 0 Gene Names: puwE
UniProt
Find proteins for A0A0A0WDC6 (Cylindrospermum alatosporum CCALA 988) Explore A0A0A0WDC6 Go to UniProtKB: A0A0A0WDC6
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	A0A0A0WDC6
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 1.60 Å
R-Value Free: 0.239
R-Value Work: 0.220
R-Value Observed: 0.221
Space Group: P 2₁ 2₁ 2

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 95.61	α = 90
b = 103.554	β = 90
c = 77.225	γ = 90

Software Package:

Software Name	Purpose
PHENIX	refinement
HKL-2000	data reduction
HKL-2000	data scaling
PHASER	phasing

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2019-09-25

Deposition Author(s):

Revision History (Full details and data files)

Version 1.0: 2019-09-25
Type: Initial release
Version 1.1: 2024-03-27
Changes: Data collection, Database references

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.