6JHZ

Crystal structure of cas2

PDB DOI: https://doi.org/10.2210/pdb6JHZ/pdb

Classification: RNA BINDING PROTEIN
Organism(s): Treponema denticola ATCC 35405, Escherichia coli
Expression System: Escherichia coli
Mutation(s): No

Deposited: 2019-02-19 Released: 2020-03-25
Deposition Author(s): Bi, M., Mo, X., Wang, C., Yuan, A.Y.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.00 Å
R-Value Free: 0.232
R-Value Work: 0.195
R-Value Observed: 0.198

wwPDB Validation 3D Report Full Report

This is version 1.1 of the entry. See complete history.

Literature

Structural insight into endonuclease CRISPR-associated Cas2 protein

Bi, M., Mo, X., Wang, C., Yuan, A.Y.

To be published.

Macromolecule Content

Total Structure Weight: 22.04 kDa
Atom Count: 1,683
Modelled Residue Count: 189
Deposited Residue Count: 191
Unique protein chains: 2

Macromolecules

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(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
CRISPR-associated endoribonuclease Cas2	A, B	93	Treponema denticola ATCC 35405	Mutation(s): 0 Gene Names: cas2, TDE_0329 EC: 3.1
UniProt
Find proteins for Q73QW4 (Treponema denticola (strain ATCC 35405 / DSM 14222 / CIP 103919 / JCM 8153 / KCTC 15104)) Explore Q73QW4 Go to UniProtKB: Q73QW4
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q73QW4
Sequence Annotations Expand
Reference Sequence

Find similar proteins by: Sequence | 3D Structure

Entity ID: 2
Molecule	Chains	Sequence Length	Organism	Details	Image
5-mer peptide	C [auth Q]	5	Escherichia coli	Mutation(s): 0
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.00 Å
R-Value Free: 0.232
R-Value Work: 0.195
R-Value Observed: 0.198
Space Group: P 2₁ 2₁ 2₁

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 26.592	α = 90
b = 76.391	β = 90
c = 88.445	γ = 90

Software Package:

Software Name	Purpose
PHENIX	refinement
HKL-2000	data reduction
HKL-2000	data scaling
MoRDa	phasing

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2020-03-25

Deposition Author(s):

Revision History (Full details and data files)

Version 1.0: 2020-03-25
Type: Initial release
Version 1.1: 2024-03-27
Changes: Data collection, Database references

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.