6HWX

Mature MLV capsid hexamer structure in intact virus particles


Experimental Data Snapshot

  • Method: ELECTRON MICROSCOPY
  • Resolution: 7.20 Å
  • Aggregation State: PARTICLE 
  • Reconstruction Method: SUBTOMOGRAM AVERAGING 

wwPDB Validation   3D Report Full Report


This is version 1.2 of the entry. See complete history


Literature

Structure and architecture of immature and mature murine leukemia virus capsids.

Qu, K.Glass, B.Dolezal, M.Schur, F.K.M.Murciano, B.Rein, A.Rumlova, M.Ruml, T.Krausslich, H.G.Briggs, J.A.G.

(2018) Proc Natl Acad Sci U S A 115: E11751-E11760

  • DOI: https://doi.org/10.1073/pnas.1811580115
  • Primary Citation of Related Structures:  
    6GZA, 6HWI, 6HWW, 6HWX, 6HWY

  • PubMed Abstract: 

    Retroviruses assemble and bud from infected cells in an immature form and require proteolytic maturation for infectivity. The CA (capsid) domains of the Gag polyproteins assemble a protein lattice as a truncated sphere in the immature virion. Proteolytic cleavage of Gag induces dramatic structural rearrangements; a subset of cleaved CA subsequently assembles into the mature core, whose architecture varies among retroviruses. Murine leukemia virus (MLV) is the prototypical γ-retrovirus and serves as the basis of retroviral vectors, but the structure of the MLV CA layer is unknown. Here we have combined X-ray crystallography with cryoelectron tomography to determine the structures of immature and mature MLV CA layers within authentic viral particles. This reveals the structural changes associated with maturation, and, by comparison with HIV-1, uncovers conserved and variable features. In contrast to HIV-1, most MLV CA is used for assembly of the mature core, which adopts variable, multilayered morphologies and does not form a closed structure. Unlike in HIV-1, there is similarity between protein-protein interfaces in the immature MLV CA layer and those in the mature CA layer, and structural maturation of MLV could be achieved through domain rotations that largely maintain hexameric interactions. Nevertheless, the dramatic architectural change on maturation indicates that extensive disassembly and reassembly are required for mature core growth. The core morphology suggests that wrapping of the genome in CA sheets may be sufficient to protect the MLV ribonucleoprotein during cell entry.


  • Organizational Affiliation

    Structural and Computational Biology Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany.


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Putative gag polyprotein
A, B, C
236Murine leukemia virusMutation(s): 0 
Gene Names: gag
UniProt
Find proteins for P03336 (AKV murine leukemia virus)
Explore P03336 
Go to UniProtKB:  P03336
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupP03336
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: ELECTRON MICROSCOPY
  • Resolution: 7.20 Å
  • Aggregation State: PARTICLE 
  • Reconstruction Method: SUBTOMOGRAM AVERAGING 
EM Software:
TaskSoftware PackageVersion
RECONSTRUCTIONAV3
RECONSTRUCTIONTOM

Structure Validation

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Entry History & Funding Information

Deposition Data


Funding OrganizationLocationGrant Number
German Research FoundationGermanyBR 3635/2-1
German Research FoundationGermanyKR 906/7-1
German Research FoundationGermanyKR 906/8-1
European Research CouncilGermanyERC-CoG-648432
Medical Research Council (United Kingdom)United KingdomMC_UP_1201/16
Czech Science FoundationCzech Republic17-25602S
Ministry of Education (Czech Republic)Czech RepublicLO1302
Ministry of Education (Czech Republic)Czech RepublicLO1304
Ministry of Education (Czech Republic)Czech RepublicLO1601

Revision History  (Full details and data files)

  • Version 1.0: 2018-12-05
    Type: Initial release
  • Version 1.1: 2018-12-19
    Changes: Data collection, Database references
  • Version 1.2: 2019-04-03
    Changes: Data collection, Source and taxonomy