6HRK

Structure of a far-red fluorescent biliprotein derived from a far-red induced allophycocyanin F subunit from a thermophilic cyanobacterium Chroococcidiopsis thermalis

PDB DOI: https://doi.org/10.2210/pdb6HRK/pdb

Classification: FLUORESCENT PROTEIN
Organism(s): Chroococcidiopsis thermalis PCC 7203
Expression System: Escherichia coli
Mutation(s): Yes

Deposited: 2018-09-27 Released: 2019-10-09
Deposition Author(s): Hou, Y.-N., Hoeppner, A., Ding, W.-L., Gaertner, W., Zhao, K.-H.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.52 Å
R-Value Free: 0.332
R-Value Work: 0.274
R-Value Observed: 0.277

wwPDB Validation 3D Report Full Report

Ligand Structure Quality Assessment

This is version 1.2 of the entry. See complete history.

Literature

Control of a far-red/near-infrared spectral switch in an artificial fluorescent biliprotein derived from allophycocyanin

Hou, Y.N., Hoppner, A., Rao, A.G., Lahav, Y., Kumar Das, P., Ding, W.L., Jiang, X.X., Hu, J.L., Schapiro, I., Noy, D., Zhao, K.H.

(2022) Protein Sci 31

DOI: https://doi.org/10.1002/pro.4412

Macromolecule Content

Total Structure Weight: 68.11 kDa
Atom Count: 4,251
Modelled Residue Count: 540
Deposited Residue Count: 588
Unique protein chains: 1

Macromolecules

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(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Allophycocyanin beta-18 subunit apoprotein	A, B, C, D	147	Chroococcidiopsis thermalis PCC 7203	Mutation(s): 18 Gene Names: Chro_1766
UniProt
Find proteins for K9TY40 (Chroococcidiopsis thermalis (strain PCC 7203)) Explore K9TY40 Go to UniProtKB: K9TY40
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	K9TY40
Sequence Annotations Expand
Reference Sequence

Small Molecules

Ligands 1 Unique
ID	Chains	Name / Formula / InChI Key	2D Diagram	3D Interactions
BLA Query on BLA Download Ideal Coordinates CCD File SDF format, chain E [auth A] SDF format, chain F [auth B] SDF format, chain G [auth C] SDF format, chain H [auth D] MOL2 format, chain E [auth A] MOL2 format, chain F [auth B] MOL2 format, chain G [auth C] MOL2 format, chain H [auth D]	E [auth A], F [auth B], G [auth C], H [auth D]	BILIVERDINE IX ALPHA C₃₃ H₃₄ N₄ O₆ GWZYPXHJIZCRAJ-SRVCBVSDSA-N		Interactions Focus chain E [auth A] Focus chain F [auth B] Focus chain G [auth C] Focus chain H [auth D] Interactions & Density Focus chain E [auth A] Focus chain F [auth B] Focus chain G [auth C] Focus chain H [auth D]

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.52 Å
R-Value Free: 0.332
R-Value Work: 0.274
R-Value Observed: 0.277
Space Group: P 1 2₁ 1

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 61.248	α = 90
b = 74.823	β = 90.67
c = 61.031	γ = 90

Software Package:

Software Name	Purpose
REFMAC	refinement
xia2	data reduction
autoPROC	data scaling
PHASER	phasing

Structure Validation

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Ligand Structure Quality Assessment

Entry History

Deposition Data

Released Date: 2019-10-09

Deposition Author(s):

Revision History (Full details and data files)

Version 1.0: 2019-10-09
Type: Initial release
Version 1.1: 2022-10-26
Changes: Database references, Refinement description
Version 1.2: 2024-01-31
Changes: Data collection, Refinement description

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Help and Resources

6HRK

Structure of a far-red fluorescent biliprotein derived from a far-red induced allophycocyanin F subunit from a thermophilic cyanobacterium Chroococcidiopsis thermalis

Control of a far-red/near-infrared spectral switch in an artificial fluorescent biliprotein derived from allophycocyanin

Entity ID: 1

UniProt

Entity Groups

Sequence Annotations

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Experimental Data

Structure Validation

Ligand Structure Quality Assessment

Deposition Data

Revision History (Full details and data files)