6FXD

Crystal structure of MupZ from Pseudomonas fluorescens

PDB DOI: https://doi.org/10.2210/pdb6FXD/pdb

Classification: BIOSYNTHETIC PROTEIN
Organism(s): Pseudomonas fluorescens
Expression System: Escherichia coli
Mutation(s): No

Deposited: 2018-03-08 Released: 2019-03-06
Deposition Author(s): Wang, L., Parnell, A., Williams, C., Bakar, N.A., van der Kamp, M.W., Simpson, T.J., Race, P.R., Crump, M.P., Willis, C.L.
Funding Organization(s): Biotechnology and Biological Sciences Research Council

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 1.45 Å
R-Value Free: 0.195
R-Value Work: 0.161
R-Value Observed: 0.162

wwPDB Validation 3D Report Full Report

This is version 1.1 of the entry. See complete history.

Literature

A Rieske oxygenase/epoxide hydrolase-catalysed reaction cascade creates oxygen heterocycles in mupirocin biosynthesis

Wang, L., Parnell, A., Williams, C., Bakar, N.A., van der Kamp, M.W., Simpson, T.J., Race, P.R., Crump, M.P., Willis, C.L.

(2018) Nat Catal

DOI: https://doi.org/10.1038/s41929-018-0183-5

Macromolecule Content

Total Structure Weight: 32.36 kDa
Atom Count: 2,143
Modelled Residue Count: 249
Deposited Residue Count: 282
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
MupZ	A, B	141	Pseudomonas fluorescens	Mutation(s): 0 Gene Names: mupZ
UniProt
Find proteins for H7CRF0 (Pseudomonas fluorescens) Explore H7CRF0 Go to UniProtKB: H7CRF0
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	H7CRF0
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 1.45 Å
R-Value Free: 0.195
R-Value Work: 0.161
R-Value Observed: 0.162
Space Group: C 1 2 1

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 42.33	α = 90
b = 80.09	β = 101.26
c = 78.82	γ = 90

Software Package:

Software Name	Purpose
GDA	data collection
PDB_EXTRACT	data extraction
PHENIX	model building
PHENIX	refinement
MOLREP	phasing
PHASER	phasing
DIALS	data scaling

Structure Validation

View Full Validation Report

Entry History & Funding Information

Deposition Data

Released Date: 2019-03-06

Deposition Author(s):

van der Kamp, M.W.

Funding Organization	Location	Grant Number
Biotechnology and Biological Sciences Research Council	United Kingdom	BB/L01386X/1

Revision History (Full details and data files)

Version 1.0: 2019-03-06
Type: Initial release
Version 1.1: 2024-01-17
Changes: Data collection, Database references, Refinement description

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.