6FUW

Cryo-EM structure of the human CPSF160-WDR33-CPSF30 complex bound to the PAS AAUAAA motif at 3.1 Angstrom resolution

Experimental Data Snapshot

  • Method: ELECTRON MICROSCOPY
  • Resolution: 3.07 Å
  • Aggregation State: PARTICLE 
  • Reconstruction Method: SINGLE PARTICLE 

wwPDB Validation   3D Report Full Report


This is version 1.2 of the entry. See complete history. 


Literature

Structural basis of AAUAAA polyadenylation signal recognition by the human CPSF complex.

Clerici, M., Faini, M., Muckenfuss, L.M., Aebersold, R., Jinek, M.

(2018) Nat Struct Mol Biol 25: 135-138

  • DOI: https://doi.org/10.1038/s41594-017-0020-6
  • Primary Citation of Related Structures:  
    6FUW

  • PubMed Abstract: 

    Mammalian mRNA biogenesis requires specific recognition of a hexanucleotide AAUAAA motif in the polyadenylation signals (PAS) of precursor mRNA (pre-mRNA) transcripts by the cleavage and polyadenylation specificity factor (CPSF) complex. Here we present a 3.1-Å-resolution cryo-EM structure of a core CPSF module bound to the PAS hexamer motif. The structure reveals the molecular interactions responsible for base-specific recognition, providing a rationale for mechanistic differences between mammalian and yeast 3' polyadenylation.

    • Organizational Affiliation: 

    Department of Biochemistry, University of Zurich, Zurich, Switzerland.


Macromolecules

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Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Cleavage and polyadenylation specificity factor subunit 11,446Homo sapiensMutation(s): 0 
Gene Names: CPSF1, CPSF160
UniProt & NIH Common Fund Data Resources
Find proteins for Q10570 (Homo sapiens)
Explore Q10570 
Go to UniProtKB:  Q10570
PHAROS:  Q10570
GTEx:  ENSG00000071894 
Entity Groups  
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UniProt GroupQ10570
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  • Reference Sequence
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Entity ID: 2
MoleculeChains Sequence LengthOrganismDetailsImage
pre-mRNA 3' end processing protein WDR33413Homo sapiensMutation(s): 0 
Gene Names: WDR33, WDC146
UniProt & NIH Common Fund Data Resources
Find proteins for Q9C0J8 (Homo sapiens)
Explore Q9C0J8 
Go to UniProtKB:  Q9C0J8
PHAROS:  Q9C0J8
GTEx:  ENSG00000136709 
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UniProt GroupQ9C0J8
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  • Reference Sequence
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(by identity cutoff)  |  3D Structure
Entity ID: 3
MoleculeChains Sequence LengthOrganismDetailsImage
Cleavage and polyadenylation specificity factor subunit 4178Homo sapiensMutation(s): 0 
Gene Names: CPSF4, CPSF30, NAR, NEB1
UniProt & NIH Common Fund Data Resources
Find proteins for O95639 (Homo sapiens)
Explore O95639 
Go to UniProtKB:  O95639
PHAROS:  O95639
GTEx:  ENSG00000160917 
Entity Groups  
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UniProt GroupO95639
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  • Reference Sequence

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Entity ID: 4
MoleculeChains LengthOrganismImage
RNA (5'-R(P*AP*AP*UP*AP*AP*AP*GP*G)-3')10unidentified adenovirus
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: ELECTRON MICROSCOPY
  • Resolution: 3.07 Å
  • Aggregation State: PARTICLE 
  • Reconstruction Method: SINGLE PARTICLE 
EM Software:
TaskSoftware PackageVersion
MODEL REFINEMENTPHENIX1.12-2829
RECONSTRUCTIONRELION2.1b1_cu8.0

Structure Validation

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Entry History & Funding Information

Deposition Data

Funding OrganizationLocationGrant Number
European Research CouncilBelgiumERC-StG-337284
European Molecular Biology OrganizationGermanyEMBO ALTF-343-2013
European Research CouncilBelgiumERC Advanced Grants no. 233226 and no. 670821
European UnionBelgiumPROSPECTS, HEALTH-F4-2008-201648

Revision History  (Full details and data files)

  • Version 1.0: 2018-03-21
    Type: Initial release
  • Version 1.1: 2019-12-11
    Changes: Other
  • Version 1.2: 2023-02-22
    Changes: Database references, Refinement description