Download Mendeley1,2-Diol Dehydration by the Radical SAM Enzyme AprD4: A Matter of Proton Circulation and Substrate Flexibility.
Liu, W.Q., Amara, P., Mouesca, J.M., Ji, X., Renoux, O., Martin, L., Zhang, C., Zhang, Q., Nicolet, Y.(2018) J Am Chem Soc 140: 1365-1371
- PubMed: 29300094
- DOI: https://doi.org/10.1021/jacs.7b10501
- Primary Citation of Related Structures:
6FD2 - PubMed Abstract:
Regiospecific dehydration of vicinal diols by enzymes is a difficult reaction that usually requires activation by dedicated organic cofactors. The enzymatic use of radical-based chemistry is an effective but challenging alternative as radical intermediates are difficult to control. Here we report the X-ray structure of the radical S-adenosyl-l-methionine (SAM) dehydratase AprD4 involved in the biosynthesis of the aminoglycoside (AG) antibiotic apramycin. Using in vitro characterizations and theoretical calculations based on our crystal structure, we have been able to propose a detailed mechanism of AprD4 catalysis, which involves a complex partially substrate-induced proton relay network in the enzyme active site and highlights the key role of the protein matrix in driving high-energy intermediates.
Organizational Affiliation:
Department of Chemistry, Fudan University , Shanghai 200433, China.
