6EKV

Deposited: 2017-09-27 Released: 2017-10-25
Deposition Author(s): Gustafsson, R., Berntsson, R.P., Stenmark, P.
Funding Organization(s): Swedish Research Council, European Molecular Biology Organization, Wenner-Gren Foundation, Swedish Cancer Society

Experimental Data Snapshot

wwPDB Validation 3D Report Full Report

This is version 1.4 of the entry. See complete history.

Literature

(2017) FEBS Lett 591: 3781-3792

PubMed Abstract:
Botulinum neurotoxins are highly toxic substances and are all encoded together with one of two alternative gene clusters, the HA or the OrfX gene cluster. Very little is known about the function and structure of the proteins encoded in the OrfX gene cluster, which in addition to the toxin contains five proteins (OrfX1, OrfX2, OrfX3, P47, and NTNH). We here present the structures of OrfX2 and P47, solved to 2.1 and 1.8 Å, respectively. We show that they belong to the TULIP protein superfamily, which are often involved in lipid binding. OrfX1 and OrfX2 were both found to bind phosphatidylinositol lipids.

Organizational Affiliation

Department of Biochemistry and Biophysics, Stockholm University, Sweden.

Macromolecule Content

Macromolecules

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(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Toxin complex component ORF-X2	A	750	Clostridium botulinum A2 str. Kyoto	Mutation(s): 0 Gene Names: CLM_0892
UniProt
Find proteins for C1FUH4 (Clostridium botulinum (strain Kyoto / Type A2)) Explore C1FUH4 Go to UniProtKB: C1FUH4
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	C1FUH4
Sequence Annotations Expand
Reference Sequence

Small Molecules

Ligands 1 Unique
ID	Chains	Name / Formula / InChI Key	2D Diagram	3D Interactions
SO4 Query on SO4 Download Ideal Coordinates CCD File SDF format, chain B [auth A] SDF format, chain C [auth A] SDF format, chain D [auth A] SDF format, chain E [auth A] MOL2 format, chain B [auth A] MOL2 format, chain C [auth A] MOL2 format, chain D [auth A] MOL2 format, chain E [auth A]	B [auth A], C [auth A], D [auth A], E [auth A]	SULFATE ION O₄ S QAOWNCQODCNURD-UHFFFAOYSA-L		Interactions Focus chain B [auth A] Focus chain C [auth A] Focus chain D [auth A] Focus chain E [auth A] Interactions & Density Focus chain B [auth A] Focus chain C [auth A] Focus chain D [auth A] Focus chain E [auth A]

Modified Residues 1 Unique
ID	Chains	Type	Formula	2D Diagram	Parent
MSE Query on MSE	A	L-PEPTIDE LINKING	C₅ H₁₁ N O₂ Se		MET

Experimental Data & Validation

Unit Cell:

Software Package:

Entry History & Funding Information

Deposition Author(s):

Gustafsson, R.

Funding Organization	Location	Grant Number
Swedish Research Council	Sweden	2016-03599
European Molecular Biology Organization	Sweden	GA-2010- 267146
Swedish Research Council	Sweden	2014-5667
Wenner-Gren Foundation	Sweden	--
Swedish Cancer Society	Sweden	--