6D25

Crystal structure of the GH51 arabinofuranosidase from Xanthomonas axonopodis pv. citri

Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 1.91 Å
  • R-Value Free: 0.167 
  • R-Value Work: 0.149 
  • R-Value Observed: 0.149 

wwPDB Validation   3D Report Full Report


This is version 1.2 of the entry. See complete history. 


Literature

The mechanism by which a distinguishing arabinofuranosidase can cope with internal di-substitutions in arabinoxylans.

Dos Santos, C.R., de Giuseppe, P.O., de Souza, F.H.M., Zanphorlin, L.M., Domingues, M.N., Pirolla, R.A.S., Honorato, R.V., Tonoli, C.C.C., de Morais, M.A.B., de Matos Martins, V.P., Fonseca, L.M., Buchli, F., de Oliveira, P.S.L., Gozzo, F.C., Murakami, M.T.

(2018) Biotechnol Biofuels 11: 223-223

  • DOI: https://doi.org/10.1186/s13068-018-1212-y
  • Primary Citation of Related Structures:  
    6D25

  • PubMed Abstract: 

    Arabinoxylan is an abundant polysaccharide in industrially relevant biomasses such as sugarcane, corn stover and grasses. However, the arabinofuranosyl di-substitutions that decorate the xylan backbone are recalcitrant to most known arabinofuranosidases (Abfs).

    • Organizational Affiliation: 

    1Brazilian Bioethanol Science and Technology Laboratory (CTBE), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, Sao Paulo 13083-970 Brazil.


Macromolecules
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(by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Alpha-L-arabinosidase
A, B, C, D, E
A, B, C, D, E, F
520Xanthomonas citri pv. citri str. 306Mutation(s): 0 
Gene Names: XAC1286
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 1.91 Å
  • R-Value Free: 0.167 
  • R-Value Work: 0.149 
  • R-Value Observed: 0.149 
  • Space Group: P 1 21 1
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 91.344α = 90
b = 163.231β = 107.57
c = 114.425γ = 90
Software Package:
Software NamePurpose
XDSdata reduction
XSCALEdata scaling
MOLREPphasing
REFMACrefinement
PDB_EXTRACTdata extraction

Structure Validation

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Entry History & Funding Information

Deposition Data

Funding OrganizationLocationGrant Number
Sao Paulo Research Foundation (FAPESP)Brazil2013/13309-0, 2014/07135-1 , 2015/26982-0

Revision History  (Full details and data files)

  • Version 1.0: 2019-02-20
    Type: Initial release
  • Version 1.1: 2020-01-01
    Changes: Author supporting evidence
  • Version 1.2: 2023-10-04
    Changes: Data collection, Database references, Refinement description