6BME

Crystal structure of Chlamydomonas reinhardtii THB4


Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 1.90 Å
  • R-Value Free: 0.227 
  • R-Value Work: 0.188 
  • R-Value Observed: 0.192 

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This is version 1.4 of the entry. See complete history


Literature

Lysine as a heme iron ligand: A property common to three truncated hemoglobins from Chlamydomonas reinhardtii.

Johnson, E.A.Russo, M.M.Nye, D.B.Schlessman, J.L.Lecomte, J.T.J.

(2018) Biochim Biophys Acta Gen Subj 1862: 2660-2673

  • DOI: https://doi.org/10.1016/j.bbagen.2018.08.009
  • Primary Citation of Related Structures:  
    6BME

  • PubMed Abstract: 

    The nuclear genome of Chlamydomonas reinhardtii encodes a dozen hemoglobins of the truncated lineage. Four of these, named THB1-4, contain a single ~130-residue globin unit. THB1, which is cytoplasmic and capable of nitric oxide dioxygenation activity, uses a histidine and a lysine as axial ligands to the heme iron. In the present report, we compared THB2, THB3, and THB4 to THB1 to gain structural and functional insights into algal globins.


  • Organizational Affiliation

    T. C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, MD 21218, United States.


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Truncated hemoglobin 4
A, B
128Chlamydomonas reinhardtiiMutation(s): 0 
Gene Names: THB4
UniProt
Find proteins for R9RY64 (Chlamydomonas reinhardtii)
Explore R9RY64 
Go to UniProtKB:  R9RY64
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupR9RY64
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 1.90 Å
  • R-Value Free: 0.227 
  • R-Value Work: 0.188 
  • R-Value Observed: 0.192 
  • Space Group: P 21 21 2
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 74.4α = 90
b = 68.3β = 90
c = 49.4γ = 90
Software Package:
Software NamePurpose
PHENIXrefinement
PDB_EXTRACTdata extraction
CrysalisProdata reduction
CrysalisProdata scaling
PHENIXphasing
Cootmodel building

Structure Validation

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Ligand Structure Quality Assessment 


Entry History & Funding Information

Deposition Data


Funding OrganizationLocationGrant Number
National Science Foundation (NSF, United States)United StatesMCB-1330488

Revision History  (Full details and data files)

  • Version 1.0: 2017-12-20
    Type: Initial release
  • Version 1.1: 2018-01-17
    Changes: Author supporting evidence
  • Version 1.2: 2019-01-16
    Changes: Data collection, Database references
  • Version 1.3: 2019-11-27
    Changes: Author supporting evidence
  • Version 1.4: 2024-03-13
    Changes: Data collection, Database references